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Method for producing polysaccharide with strong antioxidant activity through co-fermentation of somatic cell compatible ganoderma lucidum strains

An antioxidant activity, somatic cell technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of low yield, difficulty in achieving economic benefits, restricting the industrial application of Ganoderma lucidum liquid fermentation, etc. The effect of antioxidant activity

Active Publication Date: 2019-05-21
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the liquid fermentation method of Ganoderma lucidum is mainly used for the production of polysaccharides, triterpenes and mycelium of Ganoderma lucidum, and the fermentation method of single strain inoculation is used, but the yield is low, and it is difficult to achieve the economic benefits required by industrial production, which restricts the production of liquid Ganoderma lucidum. For the industrial application of fermentation, it is urgent to further develop efficient liquid fermentation methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Co-fermentation of two somatic cell-compatible Ganoderma lucidum strains inoculated at a ratio of 1:1 (weight ratio of solid strains)

[0020] 1. Somatically compatible Ganoderma lucidum strains are used as co-fermentation strains for NA8 and NA16. The mycelia of the two strains grow fast, and they have genetic differences and have different carbon sources for polysaccharide fermentation.

[0021] 2. To activate the strains, inoculate the preserved strains into the slant PDA medium, and cultivate them at 28°C for 5-7 days.

[0022] 3. Preparation of solid strains, miscellaneous wood chips were crushed with a traditional Chinese medicine grinder, and passed through a 40-mesh sieve; wood powder and soybean powder were mixed at a ratio of 80% and 20%, and the water content was adjusted to 55%, and packed into a 750 mL glass strain bottle , slightly compacted, sterilized, cooled and then inserted into the activated strain, cultured in a dark environment at 25°C and 60% rela...

Embodiment 2

[0027] Example 2 Co-fermentation of two bacterial strains inoculated at 1:0.7 (solid strain weight ratio)

[0028] 1. Strains, activation of strains and preparation of solid strains are the same as above.

[0029] 2. Co-fermentation

[0030] A 10 L fermenter was used for sterilizing at 121°C for 30 minutes, then filled with 7 L of fermentation medium and sterilized at 121°C for 30 minutes. After cooling, 50 g of solid strains of strain NA8 and 35 g of solid strains of NA16 g into the fermenter at the same time. The formula of fermentation medium is (per liter): glucose 10 g, sucrose 20 g, lactose 10 g, soybean oil 5 g, corn flour 5 g, KH 2 PO 4 0.5 g, MgSO 4 0.25 g.

[0031] The fermentation conditions were 28°C, 150 rpm, 1.0 vvm ventilation, natural pH, and cultured for 5 days.

[0032] After the fermentation, the polysaccharide concentration in the fermentation liquid was 1.08 g / L. As a control, the fermentation yield of NA8 was only 0.82 g / L, and that of NA16 was 0....

Embodiment 3

[0033] Example 3 Co-fermentation using different somatic affinity ganoderma strain pairs

[0034] 1. Using Ganoderma lucidum strain NA32 ( Ganoderma lingzhi CICC 14020) and NA39 ( Ganoderma lingzhi CICC 14045), the two strains are somatically compatible, but they have genetic differences.

[0035] 2. The methods of strain activation and solid strain preparation are the same as above;

[0036] 3. Co-fermentation

[0037]Use a 10 L fermenter, sterilize in empty space at 121°C for 30 min, then fill in 7 L of fermentation medium, and sterilize at 121°C for 30 min. 50 g into the fermenter. The formula of fermentation medium is (per liter): glucose 10 g, sucrose 20 g, maltose 10 g, soybean oil 5 g, corn flour 5 g, KH 2 PO 4 0.5 g, MgSO 4 0.25 g.

[0038] The fermentation conditions were 30°C, 160 rpm, 1.0 vvm ventilation, natural pH, and cultured for 6 days.

[0039] After the fermentation, the concentration of polysaccharides in the fermentation liquid was 1.18 g / L, ...

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Abstract

The invention discloses a method for producing polysaccharide with strong anti-oxidation activity through co-fermentation of somatic affinity ganoderma lucidum strains. The invention relates to a novel method for producing polysaccharide (extracellular) with strong antioxidant activity by simultaneously inoculating two ganoderma lucidum strains which are compatible with somatic cells but have genetic differences and different preferred carbon sources for polysaccharide fermentation into a liquid culture medium for co-fermentation. The method mainly comprises the following steps of: preparing asolid strain of a ganoderma lucidum strain pair, co-fermenting the two strains in a tank, and preparing the ganoderma lucidum polysaccharide by using a precipitation method. The antioxidant activityof the co-fermented ganoderma lucidum polysaccharide is obviously higher than that of the polysaccharide produced through single-strain fermentation, and the co-fermented ganoderma lucidum polysaccharide is suitable for the production of health-care products and medicines. Up to now, a method for producing polysaccharide by using ganoderma lucidum co-fermentation based on somatic cell affinity isnot discovered, and the method has great application and popularization prospects.

Description

technical field [0001] The invention belongs to the application field of bioengineering, and relates to a method for co-fermenting polysaccharides with strong antioxidant activity by somatic cell-affinity ganoderma lucidum strains. Background technique [0002] Ganoderma lucidum ( Ganoderma lingzhi ) is also known as Ganoderma lucidum, Xiancao, and Shenzhi. It belongs to Basidiomycetes, Agaricaceae, Polyporaceae, and Ganoderma fungus. Use history. The ancient Chinese "Shen Nong's Materia Medica" records that Ganoderma lucidum has the functions of strengthening the body, strengthening the body, nourishing and strengthening, and prolonging life. Modern studies have confirmed that Ganoderma lucidum has a wide range of pharmacological effects, such as anti-tumor, immune regulation, anti-oxidation, lowering blood fat, and lowering blood sugar. Ganoderma lucidum contains rich active ingredients, such as polysaccharides, triterpenes, sterols, nucleic acids, proteins, vitamins, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/14C12R1/645
Inventor 刘盛荣张维瑞郑世仲阮俊峰
Owner NINGDE NORMAL UNIV
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