Light-sheet microscopy for corneal nerve imaging

Abstract

The invention relates to a light-sheet microscopy for corneal nerve imaging and belongs to the technical field of corneal nerve imaging. The light-sheet microscopy comprises a control device, an illumination light path and an image acquisition light path. The illumination light path injects light into the cornea of the eye, forming a dynamic light sheet at an angle of alpha with the horizontal plane at the position of the cornea of the eye; an image signal is transmitted into the image acquisition light path at an angle of beta with the horizontal plane, and the image acquisition light path sends the image signal into the control device for imaging, wherein the angle between the angle of alpha and the angle of beta is 90 degrees, and the angle of alpha is unequal to 90 degrees. The dynamiclight-sheet microscopy is used to acquire a corneal nerve image, accelerate an imaging speed, reduce the shooting time, and avoid the injury to eyes caused by illumination light directly shining thefundus.

Description

technical field [0001] The invention relates to the field of corneal neuroimaging, in particular to corneal neuroimaging equipment using a light sheet microscope, which can be widely used in the technical field of biomedical ophthalmology imaging. Background technique [0002] The cornea is the frontmost part of the eye, in front of the iris and pupil. It is the most densely innervated tissue, and most corneal nerves are sensory nerves, derived from the thalamic branch of the trigeminal nerve. The cornea of ​​an adult eye has an average horizontal diameter of about 11.5 mm and a vertical diameter of 10.5 mm, and its curvature remains fairly constant throughout life. The optic nerve area (cornea anterior to the pupil), which provides most of the cornea's refractive function, is 4 mm in diameter and is located in the center of the cornea, in front of the pupil. Under photopic conditions, the cornea is the branch of the avascular anterior ciliary arteries that stop at where th...

AI Technical Summary

Problems solved by technology

In this image acquisition device, the illumination light path enters from the left side of the eyeball, and the image acquisition light path obtains the reflected image information from the trabecular meshwork. For animal experiments, this device may be suitable for taking out the eye-moving trabecular meshwork. But humans have left and right eyes, and there is a bridge of nose in the middle as a separation, so this kind of device is not suitable

Moreover, this lighting device uses a static light sheet. During the shooting process, the eyeball object to be photographed needs to move in conjunction with it, so as to obtain information of different depths, but it is easy to cause the problem that the moving distance does not meet the required shooting depth.

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