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Antibody for resisting staphylococcus aureus toxin and applications thereof

A staphylococcal, golden-yellow technology, applied in the direction of antibodies, antibacterial drugs, antibacterial immunoglobulin, etc., can solve tissue necrosis and other problems

Active Publication Date: 2019-05-03
SHANGHAI CHANGZHENG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at high concentrations, PVL may non-specifically adsorb to the lipid bilayer to form larger octamer pores, and when Luks-PV binds to PMNs, protein kinase A or C phosphorylates Luks-PV to activate Ca 2+ Channel, Ca 2+ Influx produces interleukins and inflammatory mediators, dissolves PMNs, releases oxidative metabolites and inflammatory mediators, triggers a series of inflammatory reactions, and causes tissue necrosis in severe cases
[0005] There is no antibody produced against Staphylococcus aureus γ-hemolysin HlgB antigen in the prior art

Method used

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  • Antibody for resisting staphylococcus aureus toxin and applications thereof
  • Antibody for resisting staphylococcus aureus toxin and applications thereof
  • Antibody for resisting staphylococcus aureus toxin and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Recombinant expression of Candida albicans HlgB protein fused to His tag

[0050] Taking the amino acids of HlgB and HlgA proteins of Staphylococcus aureus as the target sequences, the corresponding base sequences were artificially synthesized, and cloned into the Pet-21a plasmid containing the His tag by using restriction sites NdeI and XhoI. The recombinant plasmid was transformed into competent cells BL21(DE3)pLysS, and a single colony was picked and inoculated into LB liquid medium containing 100 μg / ml ampicillin the next day, and cultured overnight at 37°C with shaking. The overnight culture solution was inoculated into LB liquid medium containing 100 μg / ml ampicillin at a ratio of 1:100, and cultured with shaking at 200 rpm at 37°C until OD 600 About 0.6-0.8, add IPTG to the bacterial solution to a final concentration of 0.5mM, and induce at 37°C for 4.5h. Take the induced bacterial liquid, centrifuge at 8,000 rpm for 3 minutes to collect the bacterial...

Embodiment 2

[0055] Example 2: Recombinant expression of HlgA protein fused to His tag

[0056] Taking the amino acid of Staphylococcus aureus HlgA protein as the target sequence, the corresponding base sequence was artificially synthesized, and cloned into the Pet-21a plasmid containing His tag by using restriction sites NdeI and XhoI. The recombinant plasmid was transformed into competent cells BL21(DE3)pLysS, and a single colony was picked and inoculated into LB liquid medium containing 100 μg / ml ampicillin the next day, and cultured overnight at 37°C with shaking. The overnight culture solution was inoculated into LB liquid medium containing 100 μg / ml ampicillin at a ratio of 1:100, and cultured with shaking at 200 rpm at 37°C until OD 600 About 0.6-0.8, add IPTG to the bacterial solution to a final concentration of 0.5mM, and induce at 37°C for 4.5h. Take the induced bacterial liquid, centrifuge at 8,000 rpm for 3 minutes to collect the bacterial cells, and store at -80°C.

[0057] ...

Embodiment 3

[0061] Example 3: Recombinant expression of HlgC protein fused to His tag

[0062] Taking the amino acid of Staphylococcus aureus HlgC protein as the target sequence, the corresponding base sequence was artificially synthesized, and cloned into the Pet-21a plasmid containing His tag by using restriction sites NdeI and XhoI. The recombinant plasmid was transformed into competent cells BL21(DE3)pLysS, and a single colony was picked and inoculated into LB liquid medium containing 100 μg / ml ampicillin the next day, and cultured overnight at 37°C with shaking. The overnight culture solution was inoculated into LB liquid medium containing 100 μg / ml ampicillin at a ratio of 1:100, and cultured with shaking at 200 rpm at 37°C until OD 600 About 0.6-0.8, add IPTG to the bacterial solution to a final concentration of 0.5mM, and induce at 37°C for 4.5h. Take the induced bacterial liquid, centrifuge at 8,000 rpm for 3 minutes to collect the bacterial cells, and store at -80°C.

[0063] ...

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Abstract

The invention discloses an antibody for resisting staphylococcus aureus toxin and applications thereof, and relates to the technical field of antibodies. According to the antibody or a fragment thereof which is specifically combined with staphylococcus aureus toxin, the antibody or the fragment thereof can be specifically combined with a staphylococcus aureus gamma-hemolysin H1gB component, and can generate a cross reaction with other hemolysin components of the the hemolysin. The antibody comprises a variable region of heavy chain (VH) and a variable region of light chain (VL), wherein the variable region of heavy chain (VH) comprises the following CDR combination: VH-CDR1, VH-CDR2 and VH-CDR3, and the variable region of light chain (VL) comprises the following CDR combination: VL-CDR1, VL-CDR2 and VL-CDR3. The present invention can exert the effect of neutralizing, at least including neutralizing H1gB two-component toxin, thereby achieving the treatment of staphylococcus aureus infection alone or in combination with anti-staphylococcus aureus chemical drugs.

Description

technical field [0001] The invention relates to the technical field of antibodies, in particular to an antibody specifically binding to Staphylococcus aureus toxin. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) belongs to the genus Staphylococcus and is an important Gram-positive pathogen that can cause suppurative infections, pneumonia, pseudomembranous enteritis, pericarditis and other local infections, as well as sepsis, sepsis Syndrome and other systemic infections. Staphylococcus aureus mainly causes disease by producing toxins and invasive enzymes, including hemolysin, leukocidin, enterotoxin, coagulase, etc. Hemolysin is one of the most important virulence factors secreted by Staphylococcus aureus. Hemolysins can be divided into four types: α-hemolysin, β-hemolysin, γ-hemolysin and δ-hemolysin according to the different antigens. Among them, α-hemolysin plays an important role in the colonization and pathogenic process of Staphylococcus...

Claims

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Application Information

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IPC IPC(8): C07K16/12C12N15/13A61K39/395A61P31/04
Inventor 邹最侯炜彤郭诗雨陈思敏田复波邹泽强安毛毛
Owner SHANGHAI CHANGZHENG HOSPITAL
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