The method of using Beauveria bassiana to control Culex palustris

A technology of Beauveria bassiana and Culex palustris, applied in the field of mosquito prevention and control, can solve the problems of increasing the range of activities and increasing the difficulty of mosquito control, achieving strong resistance, enhancing the mosquito induction function, and enhancing the attracting range Effect

Active Publication Date: 2021-12-07
SHANDONG INST OF PARASITIC DISEASES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adult mosquitoes have a strong ability to travel, and their range of activities has greatly increased, which increases the difficulty of mosquito control

Method used

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  • The method of using Beauveria bassiana to control Culex palustris
  • The method of using Beauveria bassiana to control Culex palustris
  • The method of using Beauveria bassiana to control Culex palustris

Examples

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Effect test

Embodiment 1

[0026] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0027] 1) Crush 10g of kelp into 40 mesh, add 100mL of water, alternately freeze and thaw twice, extract in water at 90°C for 6 hours, and vacuum filter to obtain kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 80 mesh to obtain For fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, mix well, and inoculate with 3-5% Beauveria bassiana and cultured on a shaker at 25°C for 5-7 days to obtain a culture of Beauveria bassiana;

[0028]2) Centrifuge the culture of Beauveria bassiana and collect the sediment. Mix 30g of the Beauveria bassiana sediment with 2g of brown sugar powder, 1g of algae extract and 0.5g of yeast extract powder, and carry out at 30°C. Dry to obtain a Beauveria bassiana mixture; di...

Embodiment 2

[0033] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0034] 1) Crush 10g of kelp into 50 mesh, add 100mL of water, alternately freeze and thaw twice, extract in water at 90°C for 6 hours, and vacuum filter to obtain kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 100 mesh to obtain For fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder into 200mL of distilled water, mix well, and inoculate with 3-5% Beauveria bassiana and cultured on a shaking table at 25° C. for 7 days to obtain a culture of Beauveria bassiana; wherein, the preparation method of the algae extract is to prepare Chlorella, Anabaena, Kujin Crescent, Scenedesmus fusarium , Spleenia chrysalis, S. mulberry, Scenedesmus bulge and Scenedesmus smithii were dissolved in 75% ethanol solution, alternately frozen and thawed ...

Embodiment 3

[0040] A kind of method utilizing Beauveria bassiana to control Culex palustris, it comprises the following steps:

[0041] 1) Crush 10g of kelp into 40 mesh, add 100mL of water, freeze and thaw twice alternately, extract in water at 90°C for 6 hours, and vacuum filter to obtain the kelp filtrate; dry the leftovers of fish and shrimp, and crush them into 100 mesh to obtain Fish and shrimp powder, put 50mL of kelp filtrate, 1g of fish and shrimp powder, 1g of algae extract, 1g of yeast extract powder and 2g of activated carbon powder in 200mL of distilled water, mix evenly, inoculate with 3% Beauveria bassiana and place in Shaking bed culture was carried out at 25°C for 5 days to obtain a culture of Beauveria bassiana; wherein, the preparation method of the algae extract was to prepare Chlorella, Anabaena, Kujin Crescent, Scenedesmus fusarium, Golden Shield Dinosaurus, Esterococcus mulberry, Scenedesmus bulge and Scenedesmus smithii were dissolved in 75% ethanol solution, froze...

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Abstract

The invention discloses a method for controlling Culex palustris by using Beauveria bassiana, which comprises the following steps: 1) cultivating Beauveria bassiana to obtain a culture of Beauveria bassiana; 2) cultivating Beauveria bassiana centrifuge and collect the sediment, mix the Beauveria bassiana sediment with brown sugar powder 2g, algae extract 1g and yeast extract powder 0.5g, and dry at 30-40°C to obtain Beauveria bassiana Mixture; polylactic acid 100g was dissolved in 500mL of acetone to obtain a polylactic acid solution; Beauveria bassiana mixture, polylactic acid and urea with different contents were mixed evenly to prepare a spray film solution containing urea with different contents; 3) using Expandable polystyrene beads are used to prepare pellet cores; and the pellet cores are sprayed multiple times to obtain mosquito control particles; 4) Sprinkling the mosquito control particles into water bodies that require mosquito control, That is to realize the prevention and control of mosquitoes. The invention has the functions of trapping mosquito larvae and killing mosquitoes.

Description

technical field [0001] The invention belongs to the field of mosquito control. More specifically, the present invention relates to a method of using Beauveria bassiana to control Culex palustris. Background technique [0002] Mosquitoes belong to Diptera, Mosquitoidae, also known as mosquitoes. Mosquito bites not only cause reactions such as redness, swelling, and itching, but may also cause the spread of diseases such as malaria, dengue fever, filariasis, Zika virus disease, and Japanese encephalitis, which seriously endanger human health. The life cycle of mosquitoes can be divided into four stages, namely egg, larva, pupa and adult. Mosquitoes live in water in the first three periods, while adults live on land, and female mosquitoes need to lay eggs in stagnant water, and mosquito eggs must be in water to hatch. Adult mosquitoes have a strong ability to travel, and their range of activities has increased significantly, increasing the difficulty of mosquito control. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCA01N25/00A01N25/26A01N37/36A01N59/00A01N65/00A01N65/03A01N63/30C12N1/14C12N3/00Y02A50/30
Inventor 刘丽娟张本光公茂庆王海防程鹏刘宏美郭秀霞赵玉强王怀位
Owner SHANDONG INST OF PARASITIC DISEASES
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