A nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule, its preparation method and application

A technology of aggregation-induced luminescence and conjugated polymers, applied in the field of biomedicine, can solve the problems of limited tissue penetration depth and limited PDT applications

Active Publication Date: 2021-04-06
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, visible light limits the application of PDT in deeper tissue bacterial infections due to the limited penetration depth of white light into tissues

Method used

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  • A nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule, its preparation method and application
  • A nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule, its preparation method and application
  • A nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation process of DDNPs:

[0038] Prepared by nanoprecipitation method. First, take 200 μL of DPATP-CN (1.0 mg·mL -1 , THF), 1mLDPPT-TT (0.5mg·mL -1 , THF), 1mLPSMA (2mg·mL -1, THF) to prepare 4 mL of THF solution, mix well and add it into 10 mL of ultrapure water under ultrasonication, and ultrasonicate for 3 to 5 minutes to obtain a uniform and transparent liquid. Next, blow nitrogen into the finally obtained solution for 1 h to blow off THF. Raise the temperature to 100°C, and continue to blow nitrogen until the solution is concentrated to 5 mL. After cooling to room temperature, the solution was filtered through a 0.22 μm water filter to remove impurities.

Embodiment 2

[0040] To characterize the photothermal properties of the DDNPs prepared in Example 1, the specific steps are as follows:

[0041] Put 100 μL of LDDNPs solution in a 200 μL centrifuge tube and irradiate with 808 nm laser for 5 min with a light intensity of 600 mW cm -2 . The temperature change excited by near-infrared light was detected and recorded using a thermal imager (with an accuracy of ±0.1°C).

Embodiment 3

[0043] The photodynamic properties of the DDNPs prepared in Example 1 were characterized, and the specific steps were as follows:

[0044] Activation of reduced 2,7-dichlorofluorescein (DCFH): Take 50 μL of 10 mmol / L (4.8 mg dissolved in 1 mL of ethanol) 2,7-dichlorofluorescein diacetate (DCFH-DA) ethanol solution and add 450 μL Dilute with ethanol, then add 2mL of 0.01mol / L NaOH aqueous solution, and then place it in the dark at room temperature for 30min to activate. Then add 10 mL of 1×PBS (10 mmol / L, pH=7.4), and put the DCFH solution with a final concentration of 40 μmol / L after mixing evenly and put it on ice for use. As shown, DCFH is converted to 2,7-dichlorofluorescein (DCF, excitation 488 nm, emission 524 nm) with high quantum yield in the presence of reactive oxygen species (ROS). After adding 1 mL of activated DCFH solution and 10 μL DDNPs into a quartz cuvette and mixing them evenly, the solution was exposed to white light (5 mW·cm -2 ) under irradiation for 1 m...

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Abstract

The invention provides a nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule and its preparation method and application. It is prepared by the nanoprecipitation method of the following raw materials: the raw material includes a polymer with a structure of formula I, a structure of formula II Polymer and compound of formula III structure. Compared with the existing antibacterial technology, the present invention provides a nanoparticle (DDNPs) co-doped by a conjugated polymer and an aggregation-induced light-emitting small molecule. It synergizes photothermal and photodynamic therapy. Among them, the hydrophobic conjugated polymer DPPT‑TT with strong near-infrared light absorption and high photothermal conversion efficiency and the aggregation-induced luminescent small molecule DPATP‑CN with photodynamic activity were combined by nanoprecipitation method to prepare nano Particle DDNPs also have efficient and stable photothermal performance and excellent ROS generation ability, realizing the synergistic treatment of photothermal therapy and photodynamic therapy. DDNPs have broad-spectrum antibacterial activity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a nanoparticle co-doped with a conjugated polymer and an aggregation-induced light-emitting small molecule, and a preparation method and application thereof. Background technique [0002] A "super fungus" called Candida auris has broken out in many parts of the United States and has been listed as an "urgent threat" by the Centers for Disease Control and Prevention (CDC). Antibiotic-resistant "superbugs" and "superfungi" are emerging far faster than new drugs are being discovered. Therefore, it is urgent to develop novel and efficient antibiotics. [0003] In recent years, the potent antibacterial and anticancer activities of water-soluble conjugated polymers / small molecules have attracted extensive attention. However, due to the cumbersome preparation of water-soluble conjugated polymers / small molecules, water-soluble conjugated nanoparticles that are easy to p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/795A61K31/341A61K31/277A61P31/04A61P31/10A61K9/14A61K41/00
CPCA61K9/14A61K31/277A61K31/341A61K31/795A61K41/0052A61K41/0057A61P31/04A61P31/10A61K2300/00
Inventor 袁焕祥张红娟
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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