Method of improving identification for pseudo-ginseng medicinal material based on thin-layer chromatography

Abstract

The invention provides a method of improving identification for a pseudo-ginseng medicinal material based on a thin-layer chromatography. The method comprises the following steps that step 1), 0.5 g of medicinal material powder is taken, weighed out accurately, and placed in a conical flask with a plug, 20 mL of n-butyl alcohol saturated by water is added, after close plugging, ultrasonic processing and filtering, 40 mL of water saturated by n-butyl alcohol is added into filtered liquid, and the mixture is shaken uniformly and subjected to layering; n-butyl alcohol liquid is taken and evaporated to be dry, 1 mL of methyl alcohol is added for dissolving residues, and the mixture is taken as a test solution; step 2), a ginsenoside Rg1 reference product, a ginsenoside Re reference product, aginsenoside Rb1 reference product and a notoginsenoside R1 reference product are taken, methyl alcohol is added, and a reference product solution is obtained; step 3), according to the thin-layer chromatography method experiment, 1 microliter of the test solution and 1 microliter of the reference product solution are sucked and respectively dotted on the same silica gel G thin layer plate, dichloromethane-absolute ethyl alcohol-water-glacial acetic acid is taken as a developing agent, and the solutions are developed, taken out and dried; step 4), a color developing agent is sprayed, and heating is conducted until the developed color of spots is clear.

Description

technical field [0001] The invention belongs to the field of quality control of notoginseng medicinal materials, and in particular relates to an improved identification method of notoginseng medicinal materials based on thin-layer chromatography. Background technique [0002] Panax notoginseng is the dried root and rhizome of Panax notoginseng (Burk.) F.H.Chen, a plant of Araliaceae (Araliaceae). There are many methods for quality control of medicinal materials in the 2015 edition of "Chinese Pharmacopoeia". Among them, thin-layer identification is a color atlas identification that is easy to compare visually and visually, so it has its unique advantages. [0003] The identification method of Panax notoginseng disclosed in "Chinese Pharmacopoeia" is: take 0.5g of the powder of this product, add 5 drops of water, stir well, then add 5mL of water-saturated n-butanol, seal it tightly, shake it for 10 minutes, place it for 2 hours, centrifuge , take the supernatant, add 3 times...

AI Technical Summary

Problems solved by technology

[0005] Based on the above technical problems, the present invention provides an identification method based on TLC to improve the identification of Panax notoginseng medicinal material, which improves safety, shortens the extraction time and solves the problem that ginsenoside Re and notoginseng saponin R1 cannot be separated. The method includes the following steps:

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