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Method for measuring pyrazinamide concentration in plasma through hygroplasm combination

A technology of pyrazinamide and LC-MS, which is applied in the field of medicine, can solve the problems of the undeveloped pyrazinamide determination method, and achieve the effect of simple pretreatment method, strong specificity and high sensitivity

Inactive Publication Date: 2019-04-26
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the determination method of pyrazinamide

Method used

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  • Method for measuring pyrazinamide concentration in plasma through hygroplasm combination
  • Method for measuring pyrazinamide concentration in plasma through hygroplasm combination
  • Method for measuring pyrazinamide concentration in plasma through hygroplasm combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example: Human K 2 Determination of Pyrazinamide Concentration in EDTA Plasma

[0033] 1. Experimental materials and analytical equipment

[0034] Pyrazinamide (analyte): U.S. Pharmacoeial Convention or equivalent or higher grade standard

[0035] Pyrazinamide-d3 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0036] The reagents used are shown in Table 1 below:

[0037] Table 1 Reagent Details

[0038] Reagent name

level

manufacturer

Acetonitrile

HPLC

J. T. Baker

Methanol

HPLC

J. T. Baker

formic acid

ACS

Adamas

[0039] Note: Analytical equipment can also be used with reagents of the same level or higher level, see Table 2 below:

[0040] Table 2 Details of equipment used

[0041] components

type

manufacturer

Binary pump (binary pump)

AC Pump

AB SCIEX

Degasser

Degasser

AB SCIEX

Column oven

AC Colum...

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Abstract

The invention discloses a method for measuring pyrazinamide concentration in plasma through hygroplasm combination. A hygroplasm combination system is adopted for measurement. The method comprises thesteps that firstly, a to-be-measured sample is taken, a certain quantity of mixed organic solvent is added for conducting extraction twice, and after pretreatment, through chromatographic column separation, a mass spectrometry detector is used for detection. The method is quick to use, accurate, high in sensitivity and easy and convenient to operate, and the basis is provided for plasma concentration measurement of pyrazinamide. The linear range of a plasma standard curve is within 0.1-20 mug / mL, the within-run precision and between-run precision RSD are smaller than + / -15%, and the method issuitable for measurement of pyrazinamide concentration in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for measuring medicines, in particular to a method for measuring the concentration of pyrazinamide in blood plasma by liquid mass spectrometry. Background technique [0002] Tuberculosis is a major infectious disease that threatens human health. The drugs currently used for first-line treatment include pyrazinamide, isoniazid, rifampicin and ethanol. Among them, pyrazinamide is white or off-white crystalline powder, odorless or almost odorless, slightly bitter in taste, slightly soluble in water and very slightly soluble in ethanol. The melting point is 188-192°C, and it has a good antibacterial effect on Mycobacterium tuberculosis, and the bactericidal effect is the strongest at pH 5-5.5. The inhibitory concentration in the human body is 12.5μg / ml, up to 50μg / ml can kill Mycobacterium tuberculosis. The mechanism of action is related to pyrazinic acid. After pyrazina...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/34
CPCG01N30/34G01N30/88G01N2030/045G01N2030/342G01N2030/8822
Inventor 黄靖智
Owner 徐州立兴佳正医药科技有限公司
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