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Method for detecting pesticide residue quantity in radix ophiopogonis

A technology for pesticide residues and Ophiopogon japonicus, which is applied in the field of detection of pesticide residues in Ophiopogon japonicus, can solve problems such as endangering the health of eaters and reducing the efficacy of Ophiopogon japonicus, and achieves cost-saving experiments, short analysis time, and peak-out time fast effect

Inactive Publication Date: 2018-08-10
四川省食品药品检验检测院
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Problems solved by technology

[0003] After pesticides are applied during the growth period of Ophiopogon japonicus, pesticides will remain in Ophiopogon japonicus, which will not only reduce the efficacy of Ophiopogon japonicus, but also endanger the health of the eaters; therefore, it is necessary to detect the amount of pesticide residues in Ophiopogon japonicus , to ensure the quality and safety of Ophiopogon japonicus; however, in the prior art, due to the many types of pesticides, the pesticide residues in Ophiopogon japonicus are complicated, and there is no simple and effective method to comprehensively monitor the pesticide residues in Ophiopogon japonicus. detection, thus providing guarantee for the quality and safety of Ophiopogon japonicus

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  • Method for detecting pesticide residue quantity in radix ophiopogonis
  • Method for detecting pesticide residue quantity in radix ophiopogonis
  • Method for detecting pesticide residue quantity in radix ophiopogonis

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Embodiment 1

[0045] A method for detecting pesticide residues in Ophiopogon japonicus is provided in the present embodiment, comprising the following steps:

[0046] Step (1), the preparation of the first need testing solution;

[0047] Precisely weigh an appropriate amount of Ophiopogon japonicus to be tested, and process the Ophiopogon japonicus with QuEChERS pretreatment technology to make the first test solution;

[0048] Preferably, the QuEChERS pretreatment technology includes an extraction process and a purification process.

[0049] Further, the extraction process can be as follows: take the Ophiopogon japonicus powder to be tested, pass through a No. 3 sieve, accurately weigh an appropriate amount, put it in a plugged centrifuge tube, add an appropriate amount of glacial acetic acid solution, and vortex to completely infiltrate the Ophiopogon japonicus powder , and let it stand for a period of time; then add an appropriate amount of acetonitrile and place it on a shaker to vibrat...

Embodiment 2

[0081] This embodiment mainly investigates, after preparing the first need testing solution according to the method described in the step (1) described in implementing 1, whether there is still interference component to be tested (being Radix Ophiopogon japonicus) in the first need testing solution residual pesticide ingredients).

[0082] After a lot of research and experiments in the early stage, the pesticides contained in Ophiopogon japonicus were screened. The types of pesticides screened included prohibited and restricted pesticides, highly toxic and high Usually, it may contain some of the 107 pesticide components (some pesticide components also include one or more isomers); when detecting the pesticide residues in Ophiopogon japonicus, these 107 pesticide components can be mainly detected.

[0083] Therefore, in this example, accurately measure 107 kinds (including multiple isomers) of pesticide reference substance in an appropriate amount, and prepare a reference subs...

Embodiment 3

[0097] Methodological investigation of the present invention: linearity and range

[0098] Linearity is the basis of quantitative determination, such as impurity quantitative detection, content and dissolution methods, etc., need to verify linearity and linear range, so that within the designed measurement range, the sample concentration and response value have a linear relationship.

[0099] In this example, prepare 107 pesticide reference substance solutions (including isomers, including the first reference substance solution and the second reference substance solution) according to the method described in Example 1, and dilute to various concentration levels , sample injection respectively, adopt the detection method described in the embodiment 1 to measure, thereby measured the linear equation, linear correlation coefficient (r) and the linear range of 126 kinds of pesticide compositions respectively, shown in table 3 and table 4, wherein , r is the linear correlation coef...

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Abstract

The invention relates to a method for detecting the pesticide residue quantity in radix ophiopogonis. The method comprises the following steps of (1) weighing radix ophiopogonis to be tested; performing treatment by using a QuEChERS pretreatment technology; preparing a first test product solution; (2) weighing a proper amount of ribonolactone and sorbitol; adding acetonitrile and water for dissolution; using the materials as an analysis protection agent; (3) measuring the first test product solution; blowing the solution to the near dry state by nitrogen; adding acetone for dissolution; addingthe analysis protection agent; performing uniform mixing; using the materials as a second test product solution; (4) weighing a pesticide reference product solution; respectively adding acetonitrileto prepare a first reference product solution; measuring and taking the first reference product solution; respectively adding acetone to prepare a second reference product solution; (5) using a testing method; (6) calculating the content of pesticide in the first test product solution and the second test product solution according to an outer standard method. The method has the characteristics ofhigh repeatability, high accuracy, low utilization cost and the like. The pesticide residue quantity in the radix ophiopogonis can be fast, effectively, simply and conveniently detected.

Description

technical field [0001] The invention relates to the technical field of safety of traditional Chinese medicinal materials, in particular to a method for detecting pesticide residues in Radix Ophiopogon japonicus. Background technique [0002] With the increasing popularity of artificial planting of Chinese herbal medicines, the pesticide residues caused by the use of pesticides in the planting process have attracted widespread attention. Ophiopogon japonicus is a commonly used variety of traditional Chinese medicine in my country. The main production areas are Sichuan and Zhejiang. Through the investigation of the two production areas, it was found that the use of pesticides in the planting process of Ophiopogon japonicus mainly includes herbicides and insecticides. The planting is carried out in a crop rotation method, and there are many types of crop rotation plants, and pesticides are also used. [0003] After pesticides are applied during the growth period of Ophiopogon j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/72G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N2030/062G01N2030/146
Inventor 苟琰周娟吴强耿昭刘繁红
Owner 四川省食品药品检验检测院
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