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Method for detecting the content of lycopene

A technology of lycopene and detection method, applied in the field of food detection, can solve the problems of error in detection results, trade disputes, and difficulty in controlling stability, and achieve the effects of ensuring accuracy and stability, simple and fast operation, and obvious technological progress.

Inactive Publication Date: 2019-04-19
CHENGUANG BIOTECH GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Regarding the detection methods of lycopene content in tomato and its extracts, there are mainly spectrophotometry and high performance liquid chromatography at present, but these two methods cannot accurately quantify the lycopene content in samples.
Among them: Spectrophotometry measures the content of total carotenoids in tomato extracts, including lycopene and other carotenoid components, which cannot truly reflect the content of lycopene in samples from the principle of detection; HPLC Although chromatography can accurately locate the lycopene component, due to the stability of the lycopene standard and the difficulty of control (the rapid reaction with oxygen in the powder condition degrades, and the solution exists even at -20°C). , component conversion and degradation will occur within 24 hours), which makes it difficult to control the liquid phase lycopene detection method, and the error of the detection result reaches the level of 10%.
[0004] Due to the inaccuracy and operability of the current industry lycopene detection methods, trade disputes often occur in the industry, especially when it comes to international trade

Method used

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  • Method for detecting the content of lycopene
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  • Method for detecting the content of lycopene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: The detection method of the lycopene content adopts the following specific techniques.

[0055] (1) Weigh 5.05 g of tomato powder sample and place it in a 250 mL Erlenmeyer flask, add 100 mL of dichloromethane solution containing 0.05% BHT. Place in an ultrasonic cleaner for extraction, the ultrasonic power is 400W, the ultrasonic frequency is 40KHZ, the ultrasonic temperature is 40°C, and the ultrasonic time is 30min. After ultrasonic extraction, filter with qualitative filter paper to obtain test solution A.

[0056] (2) Pipette 2mL of solution from test solution A into a 100mL volumetric flask, add 10mL of absolute ethanol, dilute to volume with petroleum ether, mix well, and record it as test solution B. Put the test solution B in a 1cm cuvette, use petroleum ether as a blank, and measure the absorbance at 472nm to be 0.325. Bring A=0.325, K=5000 and m=5.05 into the above calculation formula (I) to obtain the total carotenoid content X in the tomato ...

Embodiment 2

[0059] Embodiment 2: The detection method of the lycopene content adopts the following specific techniques.

[0060] Except step (1), other technological process is with embodiment 1.

[0061] Step (1) is: take the tomato powder sample, add 0.05% BHT in dichloromethane solution, the ratio of solid to liquid is 1:5 (m / v). Place in an ultrasonic cleaner for extraction, the ultrasonic power is 300W, the ultrasonic frequency is 40KHZ, the ultrasonic temperature is 25°C, and the ultrasonic time is 60min. After ultrasonic extraction, filter with qualitative filter paper to obtain test solution A.

[0062] Test results: A=0.425, K=5000 and m=8.08, calculated to get X 0 It is 0.0762%; the peak area normalized ratio P=83.33%; the calculated lycopene content X is 0.0635%.

Embodiment 3

[0063] Embodiment 3: The detection method of the lycopene content adopts the following specific techniques.

[0064] Except step (1), other technological process is with embodiment 1.

[0065] Step (1) is: take the tomato powder sample, add 0.05% BHT in dichloromethane solution, the ratio of solid to liquid is 1:20 (m / v). Place in an ultrasonic cleaner for extraction, the ultrasonic power is 200W, the ultrasonic frequency is 40KHZ, the ultrasonic temperature is 50°C, and the ultrasonic time is 20min. After ultrasonic extraction, filter with qualitative filter paper to obtain test solution A.

[0066] Test results: A=0.3250.418, K=5000 and m=10.38, calculated to get X 0 is 0.0584%; the peak area normalized ratio P=86.28%; the calculated lycopene content X is 0.0504%.

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Abstract

The invention discloses a method for detecting the content of lycopene. The method comprises the following steps: (1) extracting a tomato sample to obtain a sample solution, or dissolving a tomato extract sample to obtain a sample solution, wherein the concentration of the sample solution is 0.01-0.1 mg / mL; (2) measuring the sample solution by using a spectrophotometer to obtain the content of total carotenoid in the sample; (3) measuring the sample solution by using a high performance liquid chromatographic instrument to obtain the peak area normalization ratio of lycopene, wherein the chromatographic column is Agilent ZOBAX SB-C18 and is 150mm*4.6 mm and 5[mu]m, a mobile phase condition is that methanol: dichloromethane is 95: 5, and the same detection wavelength as step (2) is adopted;(4) calculating the content of lycopene in the sample according to a calculation formula X=X0*P, wherein X is the content of lycopene in the sample; X0 is the content of total carotenoid in the sample; and P is the peak area normalization ratio of lycopene in the sample. The method can ensure the detection accuracy and stability, and is simple and fast to operate.

Description

technical field [0001] The invention belongs to the field of food detection, in particular to a method for detecting lycopene content. Background technique [0002] Lycopene is a natural food coloring, the main plant source is tomato, other types of plants such as watermelon and carrot also contain a small amount of lycopene. Lycopene is a straight-chain hydrocarbon composed of 11 conjugated and 2 non-conjugated carbon-carbon double bonds, with a molecular formula of C40H56. Lycopene is a very important natural antioxidant, its antioxidant performance is the strongest among carotenoids, and its singlet oxygen scavenging effect is 100 times that of VE. Lycopene is also a health food raw material, which has certain effects in anti-cancer and anti-cancer effects, and can effectively prevent the occurrence of prostate cancer, digestive tract cancer and cardiovascular diseases. Lycopene has been identified as a type A nutrient by the Food and Agriculture Organization of the Uni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/28G01N1/34
CPCG01N1/28G01N1/34G01N21/33
Inventor 杨清山冯默陶晓青卜丽双申亚
Owner CHENGUANG BIOTECH GRP CO LTD
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