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Fermentation method for increasing yield of L-isoleucine

A fermentation method, the technology of isoleucine, which is applied in the field of microbial fermentation, can solve the problems of limited improvement in acid production and sugar conversion rate, and achieve the effects of improving effective utilization, increasing yield, and increasing sugar-acid conversion rate

Active Publication Date: 2019-04-19
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has very limited effect on the improvement of acid yield and sugar conversion rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of fermentation method that improves L-isoleucine output

[0031] (1) Inoculate Brevibacterium lactofermentation into the seed medium for expansion to obtain seed solution 1, wherein the seed medium contains: glucose 95g / L, ammonium sulfate 10g / L, peptone 95g / L, Ammonium nitrate 8g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 2g / L, expansion temperature is 30°C, time is 18h, rotation speed is 210rpm, pH of the seed medium is 6.7, the inoculation amount is Brevibacterium lactose fermentum Bacteria powder is calculated as 0.1g / L;

[0032] (2) Inoculate Lactobacillus rhamnosus GG into the seed medium for expansion to obtain seed solution 2, wherein the seed medium contains: glucose 50g / L, yeast extract 2g / L, soybean peptone 25g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 1.2g / L, sodium chloride 0.4g / L, the expansion temperature is 32°C, the time is 12h, the rotation speed is 130rpm, the pH of the seed medium is 7.2, and the ...

Embodiment 2

[0034] Embodiment 2 A kind of fermentation method that improves L-isoleucine output

[0035] (1) Inoculate Brevibacterium lactofermentation into the seed medium for expansion to obtain seed liquid 1, wherein the seed medium contains: glucose 98g / L, ammonium sulfate 7g / L, peptone 99g / L, Ammonium nitrate 4g / L, potassium dihydrogen phosphate 4g / L, magnesium sulfate 1g / L, expansion temperature is 32°C, time is 16h, rotation speed is 290rpm, pH of the seed medium is 6.2, the inoculation amount is Brevibacterium lactose fermentum Bacteria powder is calculated as 0.1g / L;

[0036] (2) Inoculate Lactobacillus rhamnosus GG into the seed medium for expansion to obtain seed solution 2, wherein the seed medium contains: glucose 20g / L, yeast extract 8g / L, soybean peptone 15g / L, potassium dihydrogen phosphate 5g / L, magnesium sulfate 0.5g / L, sodium chloride 1.0g / L, the expansion temperature is 30°C, the time is 15h, the rotation speed is 100rpm, the pH of the seed medium is 7.0, inoculation...

Embodiment 3

[0038] Embodiment 3 A kind of fermentation method that improves L-isoleucine output

[0039] (1) Inoculate Brevibacterium lactofermentation into the seed medium for expansion to obtain seed solution 1, wherein the seed medium contains: glucose 96g / L, ammonium sulfate 9g / L, peptone 97g / L, Ammonium nitrate 6g / L, Potassium dihydrogen phosphate 3.5g / L, Magnesium sulfate 1.2g / L, expansion temperature is 31°C, time is 17h, rotation speed is 250rpm, pH of the seed medium is 6.3, the inoculum is fermented with lactose Brevibacterium powder is calculated as 0.1g / L;

[0040] (2) Inoculate Lactobacillus rhamnosus GG into the seed medium for expansion to obtain seed solution 2, wherein the seed medium contains: glucose 30g / L, yeast extract 5g / L, soybean peptone 20g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 0.8g / L, sodium chloride 0.6g / L, the expansion temperature is 31°C, the time is 14h, the rotation speed is 120rpm, the pH of the seed medium is 7.1, inoculation The am...

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PUM

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Abstract

The invention provides a fermentation method for increasing the yield of L-isoleucine. The method comprises the following steps that (1) brevibacterium lactofermentum (Brevibacterium lactofermentum) is inoculated into a seed culture medium for expansion culture to obtain a seed solution 1; (2) lactobacillus rhamnosus (Lactobacillus rhamnosus GG) inoculated into the seed culture medium for expansion culture to obtain a seed solution 2; (3) firstly, the seed solution 2 is inoculated into a fermentation culture medium, then the seed solution 1 continues to be inoculated, and fermentation cultureis carried out to obtain a fermentation liquid. The fermentation method for increasing the yield of the L-isoleucine has the advantages that through the combined use of the brevibacterium lactofermentum seed solution and the lactobacillus rhamnosus seed solution, the conversion rate of saccharic acid and the yield of the L-isoleucine are significantly increased; through the further control of theuse amount ratio of the brevibacterium lactofermentum to the lactobacillus rhamnosus in the fermentation process, the conversion rate of the saccharic acid and the yield of the L-isoleucine are increased again.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for increasing the output of L-isoleucine. Background technique [0002] The chemical name of L-Isoleucine (L-Isoleucine) is L-a-amino-β-methylpentanoic acid. It is one of the eight essential amino acids for the human body and one of the three branched-chain amino acids. It is the synthesis of human hormones, The raw material of enzymes has the effect of promoting protein synthesis and inhibiting its decomposition, and plays an important role in human life activities. In foreign countries, L-isoleucine is also widely used in lactation promotion of dairy cows, preparation of feed additives and production of functional drinks . Because of its special structure and function, L-isoleucine has a particularly important position in the metabolism of human life. [0003] There are three types of L-isoleucine production methods: extraction method, che...

Claims

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Application Information

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IPC IPC(8): C12P13/06C12P39/00C12R1/13C12R1/225
CPCC12P13/06C12P39/00
Inventor 叶朋陈峰钟迎东马婷马光辉
Owner 内蒙古拜克生物有限公司
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