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Method for producing L-theanine by fermentation method

A theanine and fermentation technology, applied in the field of microbial fermentation, can solve the problems of long production cycle and low L-theanine yield, and achieve the effects of short production cycle, simplified production process and shortened fermentation time.

Inactive Publication Date: 2019-04-16
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method has a long production cycle and low L-theanine output

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 A method for producing L-theanine by fermentation

[0033] (1) Insert Brevibacterium flavum into the seed medium for expansion to obtain seed liquid 1, the seed medium contains: glucose 20g / L, yeast powder 8g / L, ammonium sulfate 2g / L, corn steep liquor dry powder 25g / L, Potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 1.5g / L, pH 6.9, the inoculum amount calculated as Brevibacterium flavum powder is 0.1g / L;

[0034] (2) Pseudomonas nitroreductans was inserted into the seed medium for expansion to obtain seed liquid 2. The seed medium contained: glucose 20g / L, yeast powder 0.5g / L, sodium glutamate 15g / L, Magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1.5g / L, iron sodium edetate 0.05g / L, defoamer 0.3g / L, pH is 6.9, the inoculum size is based on Nitroreducens Pseudomonas Bacteria powder is calculated as 0.1g / L;

[0035] (3) Insert the seed liquid 1 and the seed liquid 2 into the fermentation medium containing matcha powder and ferment to obt...

Embodiment 2

[0038] Embodiment 2 A method for producing L-theanine by fermentation

[0039](1) Insert Brevibacterium flavum into the seed medium for expansion to obtain seed liquid 1, the seed medium contains: glucose 40g / L, yeast powder 2g / L, ammonium sulfate 8g / L, corn steep liquor dry powder 15g / L, Potassium dihydrogen phosphate 3g / L, magnesium sulfate 0.8g / L, pH 7.2, the inoculum amount is calculated as Brevibacterium flavum powder as 0.1g / L;

[0040] (2) Insert Pseudomonas nitroreductans into the seed medium for expansion to obtain seed liquid 2, the seed medium comprises: glucose 10g / L, yeast powder 1.5g / L, sodium glutamate 5g / L, Magnesium sulfate 1.5g / L, potassium dihydrogen phosphate 0.5g / L, ferric sodium edetate 0.2g / L, defoamer 0.1g / L, pH 7.3, the inoculum size is based on Nitroreducens Pseudomonas Bacteria powder is calculated as 0.1g / L;

[0041] (3) Insert the seed liquid 1 and the seed liquid 2 into the fermentation medium containing matcha powder and ferment to obtain the f...

Embodiment 3

[0044] Embodiment 3 A method for producing L-theanine by fermentation

[0045] (1) Insert Brevibacterium flavum into the seed medium for expansion to obtain seed liquid 1, the seed medium contains: glucose 30g / L, yeast powder 6g / L, ammonium sulfate 5g / L, corn steep liquor dry powder 22g / L, Potassium dihydrogen phosphate 2g / L, magnesium sulfate 1.2g / L, pH 7.1, the inoculum amount is 0.1g / L based on Brevibacterium flavum powder;

[0046] (2) Pseudomonas nitroreductans was inserted into the seed medium for expansion to obtain seed liquid 2, the seed medium comprising: glucose 15g / L, yeast powder 1.1g / L, sodium glutamate 12g / L, Magnesium sulfate 0.8g / L, potassium dihydrogen phosphate 1.2g / L, iron sodium ethylenediaminetetraacetate 0.1g / L, defoamer 0.2g / L, pH 7.1, the inoculum size is nitroreducens Pseudomonas Bacteria powder is calculated as 0.1g / L;

[0047] (3) Insert the seed liquid 1 and the seed liquid 2 into the fermentation medium containing matcha powder and ferment to ob...

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Abstract

The invention discloses a method for producing L-theanine by using a fermentation method. The method comprises the following steps of: inoculating brevibacteriumflavum into a seed culture medium for expanding culture to obtain first seed liquid; inoculating nitro-reducing pseudomonas into the seed culture medium for expanding culture to obtain second seed liquid; inoculating the first seed liquidand the second seed liquid into a fermentation culture medium containing matcha powder for fermentation to obtain first fermentation liquid, adding ethylamine into the first fermentation liquid, and continuously fermenting and culturing to obtain second fermentation liquid; adding ethylamine into the second fermentation liquid to obtain third fermentation liquid. According to the invention, the glutamine generated by microbial fermentation is directly utilized through the combined fermentation culture of the brevibacteriumflavum and the nitro-reducing pseudomonas, so that the production process is simplified, and the production cost is reduced. According to the invention, the matcha powder is added into the fermentation culture medium, and the ethylamine is added in batches in the fermentation process, so that the fermentation time is shortened, the yield of the L-theanine is improved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for producing L-theanine by fermentation. Background technique [0002] L-theanine (N-ethyl-γ-glutamine) is a unique amino acid in tea, which does not participate in protein synthesis and exists in free form, accounting for 40%-70% of the total amount of free amino acids in tea It is an effective taste substance in green tea, and its content directly determines the quality of tea. L-theanine has the effects of lowering blood pressure, anti-tumor, protecting nerves, relaxing, anti-depression, anti-fatigue, improving immunity, and reducing body fat. In the field of medicine, it can be used as antineoplastic drugs, antihypertensive drugs and sedative drugs. In the food industry, it can be used as a quality improver for tea drinks, an additive for improving food flavor and an additive for functional foods. therefore, [0003] L-theanine has beco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C12P39/00C12R1/13C12R1/38
CPCC12P13/04C12P39/00
Inventor 宋春慧陈峰郑国强刘培马光辉
Owner 内蒙古拜克生物有限公司
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