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Preparation method for upconversionnanoparticles wrapped by mesoporous silica loading indocyanine green

A technology of mesoporous silica and nanoparticles, which is applied in the fields of biotechnology and medicine, can solve the problems of unstable photodegradation, limit the application of PDT tumor treatment, etc., and achieve obvious effects

Inactive Publication Date: 2019-04-16
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of ICG, such as water instability, photodegradability, thermal degradability, and easy binding to lipoproteins, leading to rapid clearance in the body, limit its application in PDT tumor therapy.

Method used

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  • Preparation method for upconversionnanoparticles wrapped by mesoporous silica loading indocyanine green
  • Preparation method for upconversionnanoparticles wrapped by mesoporous silica loading indocyanine green
  • Preparation method for upconversionnanoparticles wrapped by mesoporous silica loading indocyanine green

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Mix the yttrium chloride hexahydrate, ytterbium chloride hexahydrate, and thulium chloride hexahydrate solutions in a volume ratio of 700:100:10, heat at 110°C for 15 minutes until it turns into a white powder, stop heating and rotate continuously, and lift Cool the flask to 50°C, add 2mL oleic acid and 10mL octadecene, heat up to 140°C, the color changes from milky white to yellow (about one hour), and cool down to the boiling point of methanol (64.7°C). Weigh 0.5M methanol solution of sodium fluoride, add it dropwise into a three-neck flask, raise the temperature to 110°C, and connect the gas circuit when there are no bubbles on the liquid surface. After evacuating for half an hour, pass nitrogen gas, raise the temperature to 260° C., and finish the reaction after one hour. Centrifuge with equal volume of ethanol, 8000 rpm for 10 min to collect the precipitate, add 20 mL of cyclohexane, centrifuge at 2000 rpm for 5 min, collect the supernatant, repeat the above steps ...

Embodiment 2

[0031]Mix the solutions of yttrium chloride hexahydrate, ytterbium chloride hexahydrate, and thulium chloride hexahydrate in a volume ratio of 800:200:40, mix well and heat at 120°C for 15 minutes until it turns into white powder, stop heating and keep rotating, lift the flask, Cool down to 50°C, add 10mL oleic acid and 40mL octadecene, raise the temperature to 180°C, the color changes from milky white to yellow (about one hour), cool down to the boiling point of methanol (64.7°C). Weigh 2.5mM sodium fluoride in methanol, add it dropwise into a three-neck flask, raise the temperature to 110°C, and connect the gas circuit when there are no bubbles on the liquid surface. After evacuating for half an hour, pass nitrogen gas, raise the temperature to 400°C, and finish the reaction after one hour. Centrifuge with equal volume of ethanol, 8000 rpm for 10 min to collect the precipitate, add 20 mL of cyclohexane, centrifuge at 2000 rpm for 5 min, collect the supernatant, repeat the ab...

Embodiment 3

[0035] Mix the solutions of yttrium chloride hexahydrate, ytterbium chloride hexahydrate, and thulium chloride hexahydrate evenly in a volume ratio of 780:200:20, heat at 110°C for 20 minutes until it turns into white powder, stop heating and keep rotating, lift the flask, Cool down to 50°C, add 6mL oleic acid and 15mL octadecene, raise the temperature to 150°C, the color changes from milky white to yellow (about one hour), cool down to the boiling point of methanol (64.7°C). Weigh 0.168g of sodium fluoride, dissolve it in 4mL of methanol, add it dropwise into a three-neck flask, raise the temperature to 110°C, and connect the gas line when there are no bubbles on the liquid surface. After evacuating for half an hour, pass nitrogen gas, raise the temperature to 300°C, and finish the reaction after one hour. Centrifuge with equal volume of ethanol, 8000 rpm for 10 min to collect the precipitate, add 20 mL of cyclohexane, centrifuge at 2000 rpm for 5 min, collect the supernatant...

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PUM

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Abstract

The invention provides a preparation method for upconversion nanoparticles wrapped by mesoporous silica loading indocyanine green. Upconversion is wrapped by mesoporous silica, and the biosecurity ofthe particles and the loading capacity of the indocyanine green are improved. The size ofthe prepared upconversion nanoparticles wrapped by the mesoporous silica loading the indocyanine green is 80-400 nanometers; after the upconversion nanoparticles wrapped by the mesoporous silica loading the indocyanine green (ICG) are added, the survival rate of cells is within the range of 85-95%; a confocalmicroscope can observe cytophagy of the particles in the cells;after particle addition through laser radiation, the death rate of the cells is within the range of 40-75%.

Description

technical field [0001] The invention belongs to the technical fields of biotechnology and medicine, and in particular relates to a method for preparing indocyanine green (ICG)-loaded mesoporous silica-wrapped upconversion nanoparticles. Background technique [0002] Indocyanine Green (ICG) is a fluorescent dye with strong absorption in the near-infrared spectrum range, low toxicity, no participation in in vivo biotransformation, and rapid excretion. It is currently the only near-infrared optical dye approved by the US FDA for clinical use. Imaging contrast enhancer. At the same time, ICG can also be used for photodynamic therapy. Under the irradiation of near-infrared light, it can selectively kill tumor tissue at a lower dose by using its high fluorescence efficiency. However, the disadvantages of ICG, such as water instability, photodegradability, thermal degradability, and easy binding to lipoproteins, leading to rapid clearance in the body, limit its application in PDT ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K49/00A61P35/00
CPCA61K9/5115A61K41/0052A61K49/0034A61K49/0093A61P35/00
Inventor 王汉杰张超楠常津张英英潘惠卓田然李显煌
Owner TIANJIN UNIV
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