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Pearl-derived functional peptide and use thereof

A technology of functional peptides and leucine, applied in the field of marine biological functional peptides, can solve problems such as limitations, restrictions on accurate quality control technology, and difficulty in improving product levels, and achieve good free radical effects

Active Publication Date: 2019-04-12
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many reports on the specific types and structures of antioxidant peptides in the enzymatic hydrolyzate, which limits the optimization of the activity of high-activity pearl cosmetic raw materials and other new pearl functional peptides, and also restricts the establishment of accurate quality control technology, resulting in product Difficult to upgrade

Method used

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  • Pearl-derived functional peptide and use thereof
  • Pearl-derived functional peptide and use thereof
  • Pearl-derived functional peptide and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Preparation of pearl enzymatic protein

[0020] 1. Raw materials and reagents

[0021] The pearl powder in the examples was provided by Guangzhou Qifu Pearl Processing Co., Ltd., passed through a 200-mesh sieve, packed in bags and sealed, and stored at -20°C.

[0022] Bromelain (enzyme activity of 400,000 U / g) was purchased from Guangbo Nanning Pangbo Bioengineering Co., Ltd.; cutinase (enzyme activity of 200,000 U / g) was purchased from Jinan Zhuodai Biotechnology Co., Ltd.; DEAE-52 Cellulose cation exchange resin was purchased from Whatman Company, and Sephadex G-15 gel resin was purchased from GE Company. Ethanol, formaldehyde, isopropanol, sodium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate and other reagents were of domestic analytical grade. The test water is ultrapure water.

[0023] 2. Pearl protein preparation

[0024] Weigh about 100g of pearl powder, add 100mL of water, 50mL of ethanol and stir well. Slowly add 3M hydr...

Embodiment 2

[0027] Example 2 Separation and Preparation of Novel Functional Peptides from Pearl Enzymolysis Protein

[0028] 1. Take 3 g of pearl hydrolyzed protein obtained from enzymatic hydrolysis in Example 1, dissolve it in 100 mL of water to make a sample solution, filter it with an ultrafiltration membrane with a molecular weight cut-off of 3 kDa, freeze-dry the retained part and the filtrate respectively, and obtain two samples: PPH- I (molecular weight >3kDa), PPH-II (molecular weight 50 = 4.5 mg / mL, EC of PPH-II 50 =40.5mg / mL, based on which the more active component PPH-I was selected for further separation.

[0029] 2. Separation of PPH-I with G15 gel column to obtain four component fragments PPH-I-1-4, after freeze-drying respectively, detect the EC of DPPH free radical scavenging activity 50 The values ​​are 7.53mg / ml, 7.33mg / ml, 5.88mg / ml, 10.461mg / ml respectively.

[0030] Described G15 gel column separation, its condition is: get PPH-I sample 1g to be dissolved in 5mL d...

Embodiment 3

[0036] Example 3. Determination of DPPH radical scavenging effect.

[0037] In this experiment, the DPPH test was used to evaluate the free radical scavenging ability of the samples. According to the literature (Hsu, K.C.; Lu, G.H.; Jao, C.L., Antioxidative properties of peptides prepared from tunacooking juice hydrolysates with orientase (Bacillus subtilis). Food Research International 2009, 42, (5-6), 647-652.) method , Determining the ability of scavenging DPPH free radical activity of pearl protein hydrolyzate. According to the preset initial concentration, take a corresponding amount of sample and dissolve it in water to obtain a sample solution. Take 100 μl of the sample solution and add it to the first row of a 96-well plate, perform concentration gradient dilution with ultrapure water, and then add 100 μl of it to a concentration of 2x10 - 4 mol / L DPPH ethanol solution, three parallel experiments were done for each concentration, and the blank test was made of absolu...

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Abstract

The present invention discloses a pearl-derived functional peptide and a use thereof. An amino acid sequence of the functional peptide is methionine-isoleucine-isoleucine-leucine-leucine-cysteine-serine-leucine-leucine (MIILLCSLL). The functional peptide has a good function of scavenging free radicals in organisms, an antioxidant function and a melanin cytochrome synthesis inhibitory activity, andthus the functional peptide can be used for preparing radical scavengers, antioxidants and cosmetic raw materials having a whitening function.

Description

technical field [0001] The invention belongs to the field of marine biological functional peptides, and in particular relates to a novel functional peptide derived from pearls and its application. Background technique [0002] Pearls have been a good product for whitening and beautifying skin since ancient times. "Compendium of Materia Medica" records that "pearls are painted on the face, making people moisturize and good color". In the modern cosmetics industry, pearls, pearl oysters and their enzymatic hydrolyzates have become important components of whitening and freckle-removing products, and have been included in the catalog of cosmetic raw materials by the State Food and Drug Administration. [0003] Converting pearl protein into functional peptide is an effective means to improve its efficacy. Modern biology, pharmacology, and medical research have found that skin color is closely related to the content, size, type and distribution of melanin in the skin, and tyrosin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P39/06A61P17/00A61K8/64A61Q19/02
CPCA61K8/64A61K38/00A61P17/00A61P39/06A61Q19/02C07K7/06
Inventor 尹浩龙丽娟李姣李茹尹团杨勇张偲
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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