Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting ingredients of Mailuoning oral liquid for clearing heat, nourishing yin, activating blood circulation and removing blood stasis

A technology of Mailuoning oral liquid and detection method, which is applied in the field of analytical chemistry, can solve problems such as affecting the separation effect, inconspicuous and undetermined peaks, complex peaks of oral liquids, etc., to achieve good separation, stable baseline, and reproducibility Good results

Active Publication Date: 2019-04-09
JINLING PHARMA
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The applicant used the research method of the fingerprint of Mailuoning Injection (CN 200410008733.6 Example 1 Chromatographic Conditions) to compare Mailuoning Oral Liquid and Mailuoning Injection, and found that except for protocatechuic acid and protocatechuic aldehyde, the peaks Except that it is not obvious and cannot be determined, the rest of the common peaks in the fingerprint of the injection can be found in the oral liquid, but the content of 5-hydroxymethylfurfural (5-HMF) and cinnamic acid in the oral liquid is less than that in the injection, and the rest The components of the oral liquid are larger than those of the injection; at the same time, because the preparation process of the Mailuoning oral liquid is different from that of the injection, the injection also contains other new components. The applicant used the chromatographic conditions of the fingerprint of the injection and found that the peak It is more complex, and some components cannot be completely separated, which obviously affects the separation effect
It shows that the current quality control technical standards can no longer meet the needs of the application and development of Mailuoning Oral Liquid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting ingredients of Mailuoning oral liquid for clearing heat, nourishing yin, activating blood circulation and removing blood stasis
  • Method for detecting ingredients of Mailuoning oral liquid for clearing heat, nourishing yin, activating blood circulation and removing blood stasis
  • Method for detecting ingredients of Mailuoning oral liquid for clearing heat, nourishing yin, activating blood circulation and removing blood stasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of reference solution: take 5-hydroxymethylfurfural, protocatechuic acid, neochlorogenic acid, protocatechualdehyde, chlorogenic acid, cryptochlorogenic acid, caffeic acid, 3,4-dicaffeoylquinic acid , 3,5-dicaffeoylquinic acid, luteolin, 4,5-dicaffeoylquinic acid, cinnamic acid, harpagoside reference substance amount, accurately weighed, with 50% methanol as solvent, respectively prepared Contains 5-hydroxymethylfurfural 81.12μg / mL, protocatechuic acid 28.625μg / mL, neochlorogenic acid 426μg / mL, protocatechualdehyde 189μg / mL, chlorogenic acid 51.3μg / mL, cryptochlorogenic acid 39.4μg / mL, caffeic acid 21μg / mL, 3,4-dicaffeoylquinic acid 179μg / mL, 3,5-dicaffeoylquinic acid 241μg / mL, luteolin 41.33μg / mL, 4,5-di A single reference solution of caffeoylquinic acid 19.8 μg / mL, cinnamic acid 465 μg / mL, and harpagoside 11.84 μg / mL.

[0049] Preparation of reference substance solution: Take an appropriate amount of chlorogenic acid reference substance, weigh it accurat...

Embodiment 2

[0057] The selection of embodiment 2 need testing solution extraction reagent

[0058] The preparation of need testing solution: get Mailuoning oral liquid (batch number 090503) 1mL, be placed in 10mL measuring bottle, add 50% methanol or distilled water to be constant to scale, shake up, cross 0.22 μ m filter membrane, get continued filtrate, namely Obtain the test solution.

[0059] Precisely draw 20 μL of the test solution, inject it into a high-performance liquid chromatograph, the chromatographic conditions are the same as in Example 1, and record the chromatograms. The HPLC chromatogram of Mailuoning Oral Liquid is shown in figure 2(S1 is distilled water, S2 is 50% methanol). The results show that, under the same concentration and the same injection volume, using distilled water as the extraction reagent, the peak area of ​​the chromatographic peak is slightly smaller, indicating that dicaffeoylquinic acids are unstable in water, but uniform in 50% methanol The prope...

Embodiment 3

[0060] Embodiment 3 Mailuoning Oral Liquid Fingerprint Research Chromatographic Column Selection

[0061] The chromatographic column of adjusting embodiment 1 is phenomenonex Gemini C 18 (Table 2 Method 2), Agilent ZORBAXSB-C 18 (Table 2 Method 3), Kromasil C 18 (Table 2 method 4), chromatogram sees respectively image 3 , Figure 4 , Figure 5 , the results show that using the column phenomenonex Luna C 18 The separation effect is the best, the baseline is flat, and the column efficiency is the highest.

[0062] Choice of mobile phase

[0063] The mobile phase of adjusting embodiment 1 is: methyl alcohol-water (table 2 method 5), acetonitrile-water (table 2 method 6), acetonitrile-0.1% phosphoric acid (table 2 method 7), methyl alcohol-0.5% phosphoric acid (table 2 method 8), methanol-0.1% formic acid (method 9 in table 2), methanol-0.1% acetic acid (method 10 in table 2) for gradient elution, the chromatograms are shown in Figure 6 , Figure 7 , Figure 8 , Figu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting ingredients of Mailuoning oral liquid for clearing heat, nourishing yin, activating blood circulation and removing blood stasis. The method comprises thefollowing steps: performing HPLC detection on a Mailuoning oral liquid test solution and a standard solution, wherein the chromatographic conditions are as follows: a C18 chromatographic column, anda column temperature of 25-40 DEG C; using methanol as a mobile phase A, using an aqueous acid solution as a mobile phase B, performing gradient elution, recording a chromatogram, calculating the similarity of a test sample by using similarity software with a fingerprint of the Mailuoning oral liquid as the reference, wherein the fingerprint of the test sample should be similar with a standard fingerprint; and calculating the contents of the ingredients in the Mailuoning oral liquid by using an external standard one point method. The method disclosed by the invention has good precision, linearrelationship, stability, repeatability, high recovery rate and good durability; the method disclosed by the invention has the advantages of good degree of separation and reproducibility of the fingerprint of the Mailuoning oral liquid, and comprehensive information, 21 common peaks are marked in total, the similarity of each batch of samples is above 0.95, and the quality of the Mailuoning oral liquid can be evaluated comprehensively, objectively and scientifically.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and particularly relates to a method for detecting components of Mailuoning oral liquid for clearing heat, nourishing yin, promoting blood circulation and removing blood stasis. Background technique [0002] The composition of traditional Chinese medicine compound is extremely complex, and it is difficult to accurately judge its inherent quality only by qualitative identification and content determination methods of index components. At the same time, a compound Chinese medicine is the result of the comprehensive action of various chemical components, so using a single component as an indicator of quality control is obviously not suitable for the quality control of compound Chinese medicine. [0003] Mailuoning Oral Liquid is an oral dosage form reformed from Mailuoning Injection. It is one of Mailuoning series products. It has the effects of clearing heat and nourishing yin, promoting blood c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/04G01N30/86G01N30/74G01N30/06G01N30/14
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/74G01N30/8634G01N30/8686G01N2030/047G01N2030/146
Inventor 周红燕张蕙汤卫国徐向阳夏云崔凌云
Owner JINLING PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com