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Application of hydrogen to preparing preparation for preventing and curing leucoderma

A technology of vitiligo and hydrogen, applied in the field of medicine, has achieved good clinical application prospects

Inactive Publication Date: 2019-04-09
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no relevant report about the use of hydrogen in the treatment of vitiligo

Method used

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  • Application of hydrogen to preparing preparation for preventing and curing leucoderma
  • Application of hydrogen to preparing preparation for preventing and curing leucoderma
  • Application of hydrogen to preparing preparation for preventing and curing leucoderma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Detection experiment of different concentrations of hydrogen on the proliferation activity of human melanocytes and keratinocytes

[0023] Experimental materials: Pig1 and Pig3V cells (gifted by Professor Caroline Le Poole, Loyola University, Chicago, USA), Pig1 is a healthy human melanocyte line, and Pig3V is a melanocyte line from a vitiligo patient. HaCaT cells are keratinocyte cell lines provided by the Central Laboratory of Huashan Hospital;

[0024] Cell incubator (Thermo Scientific 8000), hydrogen cell incubator (Shanghai Lumei Medical Technology Co., Ltd.), optical microscope (XDS-1A), CCK-8 (Beiyuntian), enzyme label detector (Thermo MK3 type), shaking Bed (Qilinbeier TS-1000);

[0025] Experimental method: When the cells grow to the logarithmic growth phase, adjust the cell density to 1×10 5 cells / mL, inoculate into 96-well plate, inoculate 200 μL per well, 37°C, 5% CO 2 Culture; the next day, the cells were given different concentrations of hydr...

Embodiment 2

[0027] Example 2. Hydrogen significantly reduces the expression levels of ROS and MDA in human melanocytes and keratinocytes under oxidative stress

[0028] Experimental materials: Pig1 and Pig3V cells and HaCaT cells are the same as in Example 1;

[0029]Reactive oxygen species detection kit (Biyuntian S0063), MDA (Nanjing Jiancheng A003-1), BCA protein quantification kit (KGDBCA). Cell incubator (Thermo Scientific 8000), hydrogen cell incubator (Shanghai Limei Medical Technology Co., Ltd.), shaker (Qilinbeier TS-1000), optical microscope (XDS-1A), fluorescence microscope (LeicaIX71), microplate reader ( DNM-9602);

[0030] Experimental group:

[0031] A. Comparison of the intervention effects of different hydrogen concentrations, the three types of cells (Pig1, Pig3V and HaCaT cells) were divided into 5 groups, the control group and the model group (melanocytes were treated with 1mM H 2 o 2 For treatment, keratinocytes were treated with 0.2mM H 2 o 2 treatment), model ...

Embodiment 3

[0036] Example 3. Hydrogen significantly improves the expression level of antioxidant reductase in melanocytes and keratinocytes

[0037] Experimental materials: melanocytes (Pig1 and Pig3V cells) and keratinocytes (HaCaT cells) are the same as in Example 1. FBS and DMEM medium were purchased from Hyclone, GPx (BioVision #K762-100), CAT (BioVision #K773-100), GSH and GSSG (BioVision #K264-100), SOD (Nanjing Jiancheng A001-3), BCA protein Quantitative kit (KGDBCA);

[0038] Cell incubator (Thermo Scientific 8000), hydrogen cell incubator (Shanghai Lumei Medical Technology Co., Ltd.), optical microscope (XDS-1A), microplate reader (DNM-9602), fluorescence chemiluminescence analyzer (ThermoFLUOROSKAN type);

[0039] Experimental grouping: Three kinds of cells Pig1, Pig3V and HaCaT cells were divided into control group, model group and 75% hydrogen intervention group. Melanocyte (Pig1 and Pig3V) model group was 1mM H 2 o 2 , 0.2 mM H for keratinocytes 2 o 2 ;

[0040] Take ...

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Abstract

The invention relates to the technical field of medicines, relates to novel medicinal application of hydrogen, and particularly relates to application of the hydrogen to preparing a preparation for preventing and curing leukoderma. The result of the cell experiment shows that the hydrogen has an excellent antioxidant effect on human melanocyte and keratinocyte, and particularly, the strength of the antioxidant protection effect of the hydrogen is positively related to the concentration of the hydrogen and the pretreatment time, so that the apoptosis of the melanocyte caused by oxidative stressis obviously reduced, and the inhibitory effects of the oxidative stress on proliferation, migration and generation of the melanocyte are alleviated. Meanwhile, the hydrogen is relatively highly safefor the human melanocyte and the keratinocyte. The hydrogen can be prepared into the clinical preparation for preventing and curing leukoderma, and the role of preventing and curing leukoderma is played through prevention and cure equipment for breathing the hydrogen, taking a hydrogen-rich bath and the like by means of orally taking a hydrogen-rich solution, intravenously injecting hydrogen-richnormal saline and the like.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of hydrogen in the preparation of preparations for preventing and treating vitiligo. Background technique [0002] The information discloses that vitiligo is a common acquired and idiopathic hypopigmentation skin disease, and its clinical features are depigmented spots caused by local loss of melanocytes. Its incidence rate is high and its spontaneous remission rate is low. have a great impact on the patient's quality of life. Studies have shown that the onset of vitiligo is usually related to trauma, sun exposure, mental trauma, pregnancy and other factors, and its specific etiology and pathogenesis are not yet clear. At present, the academic circles unanimously speculate that the pathogenesis of vitiligo may start from the oxidative stress state induced by external factors, and then promote the modification of the melanocyte protein structure to become new ant...

Claims

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Application Information

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IPC IPC(8): A61K33/00A61P17/00
CPCA61K33/00
Inventor 方伟徐金华汤芦艳康志敏孙学军
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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