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Method for preparing beta-carotene

A carotene, cellulase technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as large energy, unsatisfactory effect, and increased cost.

Active Publication Date: 2019-03-29
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The usual measure to increase dissolved oxygen is to increase blowing or forced oxygen ventilation, but this method consumes a lot of energy, increases costs, and makes it difficult for industrial production
There are also oxygen carriers n-dodecane added to the fermentation broth, but it will cause damage to the bacteria
There are also many reports of adding precursor substances, but the effect is not very satisfactory, and it will increase the cost

Method used

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  • Method for preparing beta-carotene
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1) Slant culture: prepare PDA slant medium (glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; cut potatoes into 1cm cubes, add deionized water to boil for 30min, filter with four layers of gauze after cooling, Take the filtered supernatant and add glucose and agar powder). Take the spore suspensions of B. trispora positive bacteria and B. trispora negative bacteria, spread them on the PDA slant medium respectively, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0051] 2) Seed culture: Shovel a shovel of positive bacteria and negative bacteria from the slant surface of the positive bacteria and negative bacteria strains of B. trispora respectively, and inoculate them into 1000ml triangular flasks containing 150ml seed culture medium respectively. Cultivate for 48 hours under the condition of 25°C and 180 rev / min, to obtain positive bacteria seed liquid of B. trispora and negative bacteria seed liquid of B. trispora. The seed mediu...

Embodiment approach 9

[0066] Present embodiment scheme nine (D9): use 0.1% pectinase (50000U / g), cellulase (20000U / g), neutral protease (100000U / g) respectively with the positive and negative seed liquid of the three spora boulardii g) and lipase (10000U / g) in a mass ratio of 4:3:2:2 compound enzyme, treated at pH 6.5, 30°C for 2h, then mixed for fermentation, and the β- The yield of carotene is 10.33g / L, which is 102.95% higher than that of the prior art.

Embodiment 2

[0068] 1) Slant culture: prepare PDA slant medium (glucose 20g / L, agar powder 25g / L, peeled potatoes 200g / L; cut potatoes into 1cm cubes, add deionized water to boil for 30min, filter with four layers of gauze after cooling, Take the filtered supernatant and add glucose and agar powder). Take the spore suspensions of B. trispora positive bacteria and B. trispora negative bacteria, spread them on the PDA slant medium respectively, and cultivate them in a constant temperature incubator at 25°C for 5-7 days;

[0069] 2) Seed culture: Shovel a shovel of positive bacteria and negative bacteria from the slant surface of the positive bacteria and negative bacteria strains of B. trispora respectively, and inoculate them into 1000ml triangular flasks containing 150ml seed culture medium respectively. Cultivate for 48 hours under the condition of 25°C and 180 rev / min, to obtain positive bacteria seed liquid of B. trispora and negative bacteria seed liquid of B. trispora. The seed mediu...

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Abstract

The invention relates to a method for preparing beta-carotene: in seed stage, blakeslea trispora positive bacteria and blakeslea trispora negative bacteria are respectively treated through enzymolysis; then the positive bacteria and negative bacteria are mixed according to certain ratio to perform fermentation cultivation; after the fermentation, produced thalluses are collected, and the beta-carotene is extracted therefrom. The enzyme is one, or is a compound enzyme formed by optionally mixing any two to four, of pectinase, cellulase, neutral protease or lipase. According to the modified technology, the seed liquids of blakeslea trispora positive bacteria and blakeslea trispora negative bacteria are treated through enzymolysis, so that by means of reducing adhesion and winding of the mycelia in the thallus, the dispersion degree of the mycelia is increased; when the thalluses are mixed, conjugation efficiency between the positive bacteria and negative bacteria is improved and zygospore yield is increased, thereby increasing yield of the beta-carotene.

Description

technical field [0001] The invention relates to a method for preparing β-carotene, in particular to a method for preparing β-carotene by using B. trispora. Background technique [0002] β-carotene is a kind of fat-soluble carotenoid, which has good anti-oxidation, anti-cancer and anti-cancer effects, and has certain effects on preventing cardiovascular diseases, enhancing the body's immunity and delaying aging. It is a very A functional natural pigment with development value. [0003] The production of β-carotene raw materials mainly includes natural extraction method, chemical synthesis method and microbial fermentation method. The raw materials required by the natural extraction method are mainly carrots, tomatoes and other plants. These plants are limited by climate, place of origin, transportation and other conditions, and the plant extraction process is cumbersome and lengthy, and the cost is expensive, which cannot meet the needs of industrial production. Although th...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 汪志明陆姝欢杨艳红余超李翔宇
Owner CABIO BIOTECH WUHAN CO LTD
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