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A kind of preparation method and application of semi-dry nitrifying bacteria agent

A technology of nitrifying bacteria and nitrifying bacteria, which is applied in the field of preparation of semi-dry nitrifying bacteria, can solve the problems of nitrification system collapse, harsh living conditions, slow growth, etc., and achieve strong stress resistance, good stability and convenient storage and transport effects

Active Publication Date: 2022-07-26
山东海景天环保科技股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitrifying bacteria are strictly aerobic autotrophic bacteria with harsh living conditions and slow growth. They are often impacted in sewage treatment biochemical systems where water quality and quantity fluctuate frequently, causing the nitrification system to collapse.

Method used

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  • A kind of preparation method and application of semi-dry nitrifying bacteria agent
  • A kind of preparation method and application of semi-dry nitrifying bacteria agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] For the preparation of a semi-dry nitrifying bacterial agent, take 20L of activated sludge from the aerobic tank of a sewage treatment plant in normal operation, let it stand for 2 hours, discard the supernatant, and then put the precipitated activated sludge at a rotating speed of 2000r / min. Centrifuge for 2 min, discard the supernatant to obtain concentrated activated sludge with a volume of 1 L;

[0040] Prepare 9L special inorganic salt medium for nitrifying bacteria, the formula is: sodium bicarbonate 1.0g / L, dipotassium hydrogen phosphate trihydrate 1.0g / L, potassium dihydrogen phosphate 1.0g / L, sodium chloride 0.1g / L, seven Water ferrous sulfate 0.02g / L, anhydrous magnesium sulfate 0.02g / L, copper sulfate 0.02g / L, ammonium sulfate 0.5g / L, and water to make up the balance.

[0041] The concentrated activated sludge is added to the culture medium to start the enrichment culture of nitrifying bacteria. The enrichment culture method is as follows: use an electromagn...

Embodiment 2

[0047] For the preparation of a semi-dry nitrifying bacterial agent, take 50L of activated sludge from the aerobic tank of a sewage treatment plant in normal operation, let it stand for 2 hours, discard the supernatant, and then put the precipitated activated sludge at a rotating speed of 3000r / min. Centrifuge for 3 min, discard the supernatant to obtain concentrated activated sludge with a volume of 3 L;

[0048] Prepare 7L special inorganic salt medium for nitrifying bacteria, the formula is: sodium bicarbonate 1.2g / L, dipotassium hydrogen phosphate trihydrate 1.5g / L, potassium dihydrogen phosphate 1.3g / L, sodium chloride 0.4g / L, seven Water ferrous sulfate 0.02g / L, anhydrous magnesium sulfate 0.04g / L, copper sulfate 0.02g / L, ammonium sulfate 2.0g / L.

[0049] The concentrated activated sludge is added to the culture medium to start the enrichment culture of nitrifying bacteria. The enrichment culture method is as follows: aeration of this 10L medium with an electromagnetic ...

Embodiment 3

[0056] For the application of a semi-dry nitrifying bacterial agent, the nitrifying bacterial agent prepared in Example 1 and Example 2 was stored for 12 months and taken out, and a certain tannery sewage was taken as the treatment object to verify the nitrification effect of the bacterial agent. Three aerobic systems were established respectively, 10L of tannery sewage was added respectively, and an electromagnetic oxygen pump was used for aeration to maintain dissolved oxygen at 4.0-6.0mg / L; pH was maintained at 7.5-8.0.

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Abstract

The invention belongs to the technical field of biology and water treatment, and provides a preparation method and application of a semi-dry state nitrifying bacterial agent. (2) After enrichment culture, carry out domestication culture to obtain a culture solution with high nitrification activity; (3) Concentrate the culture solution and place it at a certain concentration (4) Mix (3) the mixed solution with the treated carrier to obtain a bacterial agent; (5) put the bacterial agent into a vacuum-sealed bag, vacuum seal, and place it in a cool place Store at room temperature. The bacterial agent only needs to be dispersed with water, and then added to the aerobic biochemical system. It has the characteristics of high activity, high tolerance, good stability, strong resistance to stress, etc., and is convenient for transportation and storage. , small footprint and other advantages; no activation and rejuvenation are required during application.

Description

technical field [0001] The invention belongs to the technical field of biology and water treatment, and provides a preparation method and application of a semi-dry nitrifying bacterial agent. Background technique [0002] Ammonia nitrogen has become one of the common pollutants in the water environment, which endangers the safety of aquatic organisms and even the entire ecosystem. There are physical, chemical and biological methods for denitrification. Biological methods are widely used in sewage treatment due to their advantages of simple operation, low investment, low cost, no secondary pollution and good treatment effect. Nitrifying bacteria are the main microorganisms in the process of biological denitrification, and the stability of nitrification and the improvement of nitrification rate are the keys to affect the denitrification efficiency of the whole system. Nitrifying bacteria are strictly aerobic and autotrophic bacteria with harsh living conditions and slow growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/20C12N1/02
CPCC12N1/02C12N1/20C12N1/36
Inventor 王珍马韵升栾波张映徐泽平吴文雷杨传伦车树刚薛飞
Owner 山东海景天环保科技股份公司
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