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Probe based on mesoporous nano carbon sphere doped gold nanoparticle material and preparation method of probe

A technology of gold nanoparticles and nanocarbon spheres, which is applied in the field of cancer cell diagnosis and treatment probes, can solve the problems of limited use, difficulty in metabolism and liver toxicity, and achieve the effect of preventing coagulation

Active Publication Date: 2019-03-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common inorganic materials are quantum dots and some other fluorescent dyes, but these molecules are often not easy to metabolize, resulting in certain liver toxicity, which limits their use in vivo

Method used

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  • Probe based on mesoporous nano carbon sphere doped gold nanoparticle material and preparation method of probe
  • Probe based on mesoporous nano carbon sphere doped gold nanoparticle material and preparation method of probe
  • Probe based on mesoporous nano carbon sphere doped gold nanoparticle material and preparation method of probe

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Experimental program
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Effect test

Embodiment 1

[0048] 1. Reagents

[0049] In this example, all the chemical reagents used were of analytical grade or above. Chlorauric acid (HAuCl 4 ·4H2O), formaldehyde (37wt.%) and phenol were purchased from Shanghai Titan Technology Co., Ltd. F127 (Mw=12,600, PEO106PPO70PEO106) was purchased from Acros Company. Hydrochloric acid (HCl, 37%), concentrated sulfuric acid (H 2 SO 4 ,98%), concentrated nitric acid (HNO 3 ,≥97.2%), folic acid (FA), dimethylsulfoxide (DMSO) and sodium borohydride (NaBH 4 ) were purchased from Sinopharm Co., Ltd. N-hydroxysuccinimide (NHS), bovine serum albumin (BSA, 99.9%) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were purchased from Aladdin Ltd. Apoptosis Detection Kit, 4',6-diamidino-2-phenylindole dihydrochloride (DAPI), CCK-8 kit (CCK-8) and Calcein-AM / PI Live / Dead Cell double staining kits were purchased from Yisheng Co., Ltd. Fetal bovine serum albumin (FBS), cell culture medium (DMEM), and trypsin digest were purchase...

Embodiment 2

[0067] Adopt the preparation step identical with embodiment 1, difference is:

[0068] (1) When preparing mesoporous carbon nanospheres doped with gold nanoparticles composites: the mass ratio of phenol, formaldehyde and sodium hydroxide is 0.5:2:5, and the mixing temperature of phenol, formaldehyde and sodium hydroxide is 65°C , the stirring time is 40min; in the aqueous solution of polyether F127 added, the ratio of the mass of polyether F127 to the mass of phenol is 1.5:1, and the temperature when adding the aqueous solution of polyether F127 is 60°C, and the stirring time is 4h ; Added 3-mercaptopropyltrimethoxysilane, HAuCl 4 1. The mass ratio of pure water to phenol is 60 6:20; the reaction temperature is 65°C, and the reaction time is 18h; the hydrothermal reaction temperature is 120°C, and the reaction time is 30h; washing is carried out alternately with ethanol and pure water , the number of washings is more than 3 times; the drying temperature is 50°C, and the dryin...

Embodiment 3

[0075] Adopt the preparation step identical with embodiment 1, difference is:

[0076] (1) When preparing mesoporous carbon nanospheres doped with gold nanoparticles composites: the mass ratio of phenol, formaldehyde and sodium hydroxide is 1:1:10, and the mixing temperature of phenol, formaldehyde and sodium hydroxide is 75°C , the stirring time is 20min; in the aqueous solution of polyether F127 added, the ratio of the mass of polyether F127 to the mass of phenol is 2.5:0.5, and the temperature when adding the aqueous solution of polyether F127 is 70°C, and the stirring time is 2h ; Added 3-mercaptopropyltrimethoxysilane, HAuCl 4 1. The mass ratio of pure water to phenol is 65:4:30; the reaction temperature is 70°C, and the reaction time is 12h; the hydrothermal reaction temperature is 150°C, and the reaction time is 20h; ethanol and pure water are used for washing alternately Washing, the number of washings is more than 3 times; the drying temperature is 80°C, and the dryi...

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Abstract

The invention relates to a probe based on a mesoporous nano carbon sphere doped gold nanoparticle material and a preparation method of the probe. The probe comprises a mesoporous carbon nanosphere, agold nanoparticle growing on the inner orifice wall of the mesoporous carbon nanosphere, an organic fluorescent substance supported in an orifice of the mesoporous carbon nanosphere and a targeting molecule connected to the surface of the mesoporous carbon nanosphere by a chemical bond. Compared with the prior art, the probe can both realize diagnostic monitoring and serve as a therapeutic probe,and has good targeting performance and excellent therapeutic effects based on PTT and PDT. Finally, the synthesized probe successfully ablates tumors (tumors of tumor-bearing mice with MGC-803 cells)and performs fluorescence imaging in vivo and in vitro. In addition, the gold particle located inside the mesoporous carbon nanosphere is not only well protected, but also fully exerts the catalytic effect of a nano-enzyme.

Description

technical field [0001] The invention relates to the technical field of cancer cell diagnosis and treatment probes, in particular to a probe based on mesoporous nanocarbon spheres doped with gold nanoparticles and its preparation. Background technique [0002] As we all know, cancer is a major disease with high morbidity and mortality in the whole country and even in the world, which poses a great threat to human life and health safety. Cancer is a major killer that threatens human life and health, and it is also a major problem in the medical field. Early diagnosis of cancer is of great significance to the treatment of tumors, and early precision treatment can achieve good therapeutic effects on tumors. The current clinical diagnostic technology is still insufficient, and the early diagnosis effect is not ideal. The lack of early diagnosis methods leads to unsatisfactory diagnosis and treatment of tumor patients, so finding a technology that can accurately diagnose early i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K47/04A61K47/64A61K47/54A61K41/00A61P35/00
CPCA61K41/0052A61K49/0021A61K49/005A61K49/0052A61K49/0056A61P35/00
Inventor 崔大祥章阿敏潘少君常杰黄志成
Owner SHANGHAI JIAO TONG UNIV
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