Amorphous calcium phosphate-polyacrylic acid hybridized nano material and preparation method and application thereof

A nanomaterial, crystalline calcium phosphate technology, applied in the field of biomedical materials, can solve the problems of non-degradable and poor biocompatibility, and achieve the effects of improving stability, enhancing development, and degrading quickly

Active Publication Date: 2019-03-01
INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of poor biocompatibility and inability to degrade in the body of the existing fluorescent reagent carrier, and to provide an amorphous calcium phosphate-polyacrylic acid hybrid nanomaterial and its preparation method and application. The material has good biocompatibility Non-toxic, degradable, can be used as a carrier of fluorescent molecules

Method used

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  • Amorphous calcium phosphate-polyacrylic acid hybridized nano material and preparation method and application thereof
  • Amorphous calcium phosphate-polyacrylic acid hybridized nano material and preparation method and application thereof
  • Amorphous calcium phosphate-polyacrylic acid hybridized nano material and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Solution preparation: weigh 240mg of Ca(NO 3 ) 2 4H 2 O and 36mg of Mg(NO 3 ) 2 ·6H 2 O was dissolved in 50 mL of deionized water, and stirred evenly to obtain a mixed solution A. Weigh 110mg of (NH 4 ) 2 HPO 4 and 40 mg of PAA were dissolved in 200 mL of deionized water, stirred evenly to obtain a mixed solution B, and ammonia water was added drop by drop to adjust the pH to 9.5.

[0040] Wet chemical precipitation: Add the mixed solution A prepared in the previous step into the mixed solution B dropwise at a rate of 1 mL / min. Stir magnetically at 500 rpm for 1 hour.

[0041] Cleaning: after the reaction, centrifuge at 4000rpm. After centrifugation, remove the supernatant, collect the precipitate, add 50 mL of deionized water, and ultrasonically disperse to obtain a dispersion.

[0042] Dialysis: Inject the dispersion into a 8000-14000Da dialysis membrane, perform dialysis for 24 hours, and change the water every 6 hours. The goal is to remove unwrapped pol...

Embodiment 2

[0046] Solution preparation: Weigh 267mg Ca(NO 3 ) 2 4H 2 O was dissolved in 50 mL of deionized water, and stirred evenly to obtain a mixed solution A. Weigh 110mg (NH 4 ) 2 HPO 4 and 40 mg of PAA were dissolved in 200 mL of deionized water, stirred evenly to obtain a mixed solution B, and ammonia water was added drop by drop to adjust the pH to 9.5.

[0047] Wet chemical precipitation: Add the mixed solution A prepared in the previous step to the mixed solution B dropwise at a rate of 1 mL / min. Stir magnetically at 500 rpm for 1 hour.

[0048] Cleaning: after the reaction, centrifuge at 4000rpm. After centrifugation, remove the supernatant, collect the precipitate, add 50 mL of deionized water, and ultrasonically disperse to obtain a dispersion.

[0049] Dialysis: Inject the dispersion into a 8000-14000Da dialysis membrane, perform dialysis for 24 hours, and change the water every 6 hours. The goal is to remove unwrapped polyacrylic acid molecules.

[0050] Drying: ...

Embodiment 3

[0052] Solution preparation: Weigh 227mg Ca(NO 3 ) 2 4H 2 O and 54mg Mg(NO 3 ) 2 ·6H 2 O was dissolved in 50 mL of deionized water, and stirred evenly to obtain a mixed solution A. Weigh 110mg (NH 4 ) 2 HPO 4 and 40 mg of PAA were dissolved in 200 mL of deionized water, stirred evenly to obtain a mixed solution B, and ammonia water was added drop by drop to adjust the pH to 9.5.

[0053] Wet chemical precipitation: Add the mixed solution A prepared in the previous step to the mixed solution B dropwise at a rate of 1 mL / min. Stir magnetically at 500 rpm for 1 hour.

[0054] Cleaning: after the reaction, centrifuge at 4000rpm. After centrifugation, remove the supernatant, collect the precipitate, add 50 mL of deionized water, and ultrasonically disperse to obtain a dispersion.

[0055] Dialysis: Inject the dispersion into a 8000-14000Da dialysis membrane, perform dialysis for 24 hours, and change the water every 6 hours. The goal is to remove unwrapped polyacrylic ac...

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Abstract

The invention discloses an amorphous calcium phosphate-polyacrylic acid hybridized nano material and a preparation method and application thereof, and belongs to the field of biomedical materials. Under synergistic stable effects of polyacrylic acid molecules and magnesium ions, the amorphous calcium phosphate-polyacrylic acid hybridized nano material is prepared by a wet chemistry method. The doped polyacrylic acid molecules can be complexed with calcium ions, the polyacrylic acid molecules and magnesium ions jointly stop formation of crystals. Compared with an existing calcium phosphate crystal material, the amorphous calcium phosphate-polyacrylic acid hybridized nano material is degraded more quickly, and can be used for coated carriers of functional molecules such as genes and drugs. For example, if the amorphous calcium phosphate-polyacrylic acid hybridized nano material coats fluorescent molecules (such as fluorescein isothiocyanate and indocyanine green) in a hybrid material, afluorescent developing effect can be achieved. Compared with a traditional fluorescent developing agent, the amorphous calcium phosphate-polyacrylic acid hybridized nano material coats the fluorescentmolecules, has a function of rapidly releasing the fluorescent molecules in a weak acid environment, and is a fluorescent carrier with quite good biological compatibility.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an amorphous calcium phosphate-polyacrylic acid hybrid nanomaterial and its preparation method and application. Background technique [0002] Biomedical hybrid materials combine the respective functions of organic molecules and inorganic materials, and have highlighted their increasingly important research value in biomedical materials. At present, nano-hybrid materials are widely used in gene carriers, drug carriers, and tooth restoration and regeneration [Document 1: Wu, Y. et al., Devising new lipid-coated calcium phosphate / carbonate hybrid nanoparticles to control release in endosome efficient gene delivery.J.Mater.Chem.B,2017,5(34):p.7194-7203. Document 2: Sailor,M.et al.,Hybrid Nanoparticles for Detection and Treatment of Cancer.Adv.Mater.,2012,24 (28): p.3779-3802. Document 3: Elsharkawy, S. et al., Protein disorder–order interplay to guide the growth of hier...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L33/02C08K3/32A61K49/00
CPCA61K49/0054C08K3/32C08K2003/325C08L2203/02C08L33/02
Inventor 张兴崔嵬李娜杨锐
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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