Characteristic fingerprint spectrum of Pheretim or processed products thereof, construction method therefor and application thereof

A technology of characteristic fingerprints and construction methods, which is applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as unseen, and achieve the effects of simple operation, effective quality monitoring, and accurate results.

Active Publication Date: 2019-02-12
广州白云山中药饮片有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports of HPLC fingerprints of Dilong, but there are no reports of fingerprints of Dilong processed from licorice at home and abroad.

Method used

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  • Characteristic fingerprint spectrum of Pheretim or processed products thereof, construction method therefor and application thereof
  • Characteristic fingerprint spectrum of Pheretim or processed products thereof, construction method therefor and application thereof
  • Characteristic fingerprint spectrum of Pheretim or processed products thereof, construction method therefor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 The construction method of Guangdilong raw product or its processed product fingerprint

[0051] 1. Test materials and instruments

[0052] (1) Material

[0053] Hypoxanthine (20161219-2, Baoji Chenguang Biotechnology Co., Ltd.), inosine (140669-201606, China Institute for Food and Drug Control), uracil (100469-201302, China Institute for Food and Drug Control). The sources of the 11 batches of Cantonis chinensis products are shown in Table 1, and the sources of 11 batches of licorice-processed Cantonis chinensis products are shown in Table 2, and they were identified by Associate Professor Gao Ming (Guangzhou University of Traditional Chinese Medicine). Dried body of Pheretima aspergillum (E.Perrier).

[0054] Table 1

[0055]

[0056]

[0057] Table 2

[0058] Numbering

Processed product batch number

Production Date

1

YPA7J0001

2017.10

2

YPA7J0002

2017.10

3

YPA7J0003

2017.10

4

YPA...

Embodiment 2

[0103] A kind of embodiment of the construction method of the feature fingerprint of Guangdilong or its processed product of the present invention, comprises the following steps:

[0104] (1) Preparation of the test product solution: crush the processed product of Guangdilong or its raw product through an 80-mesh sieve, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 10 mL of 0.9% NaCl solution, and extract by ultrasonic for 20 min. 5000r·min -1 Centrifuge for 10min, take the supernatant, dilute to 10mL with 0.9% NaCl solution, and filter to get it. The mass volume ratio of the raw product of Guangdilong or its processed product and the NaCl solution in the test solution is 1:20.

[0105] (2) Preparation of reference substance solution: accurately weigh hypoxanthine, inosine, uracil reference substance, add 0.9% NaCl solution to dissolve, as reference substance solution, the concentration of hypoxanthine in the described reference substance solution is 80 μ g / ...

Embodiment 3

[0117] A kind of embodiment of the construction method of the feature fingerprint of Guangdilong or its processed product of the present invention, comprises the following steps:

[0118] (1) Preparation of the test solution: crush the processed product of Guangdilong or its raw product through an 80-mesh sieve, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 10mL of 0.9% NaCl solution, and ultrasonically extract for 20min, 5000r min -1 Centrifuge for 10min, take the supernatant, dilute to 10mL with 0.9% NaCl solution, and filter to get it. The mass volume ratio of the raw product of Guangdilong or its processed product and the NaCl solution in the test solution is 1:20.

[0119] (2) Preparation of reference substance solution: accurately weigh hypoxanthine, inosine, uracil reference substance, add 0.9% NaCl solution to dissolve, and as reference substance solution, the concentration of hypoxanthine in the reference substance solution is 95 μ g / ml , The conce...

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Abstract

A method for constructing a characteristic fingerprint spectrum of Pheretim or processed products thereof according to the invention, comprising the following steps of: (1) preparing a test product solution: crushing Pheretim raw product or processed products thereof, adding a NaCl solution, carrying out ultrasonic extraction, centrifuging, taking supernate, diluting to volume, and filtering, so as to obtain a test product solution; (2) preparing a control substance solution: taking hypoxanthine, inosine and uracil control substance, adding the NaCl solution and dissolving to obtain a controlsubstance solution; and (3) carrying out high performance liquid chromatograph detection, so as to construct the characteristic fingerprint spectrum of the Pheretim raw product or processed products thereof. According to the characteristic fingerprint spectrum of the Pheretim or the processed products thereof, construction method therefor and application thereof, hypoxanthine, inosine and uracil are adopted as control substance solution and a main detection index, so that hybrid peaks in the elution process can be effectively detected, and the Pheretim raw product and processed products can beaccurately calculated. The method disclosed by the invention has the advantages of simple operation, stability and high repeatability. According to the method, more complete quality evaluation can becarried out on the Pheretim and the processed products thereof, and the Pheretim and the processed products thereof can be quickly and accurately identified.

Description

technical field [0001] The invention relates to a characteristic fingerprint spectrum of Guangdilong or its processed product and its construction method and application, belonging to the technical field of traditional Chinese medicine detection. Background technique [0002] Guangdilong is the dried body of Pheretima aspergillum (E.Perrier), an animal of the genus Pheretima. It has the functions of clearing away heat and calming convulsions, dredging collaterals, relieving asthma, and diuresis. It is used for high fever, coma, convulsions, arthralgia, numbness of limbs, hemiplegia, cough due to lung heat, edema and oliguria. [0003] The processing method and processing effect of Radix Glycyrrhizae are recorded in the first volume of the 2011 edition of the "Guangdong Provincial Traditional Chinese Medicine Processing Standards" and the first volume of the 1994 edition of "Guangdong Traditional Chinese Medicine", but in the "Guangdong Provincial Standards of Chinese Medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/88G01N2030/062G01N2030/027G01N2030/8809
Inventor 刘国伟吴志坚罗文英孔箭刘劲涛黄玉梅陈俊禧朱启亮马恩耀严冬慧
Owner 广州白云山中药饮片有限公司
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