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Preparation method of low-toxicity ginkgo ketone ester composition

A technology of ginkgo ketone ester and composition, which is applied in the field of preparation of low-toxic ginkgo ketone ester composition, can solve problems such as unfavorable and accurate drug use, and lower content range, so as to improve the safety of drug administration, reduce the content of toxic components, and improve the content range. precise and stable results

Inactive Publication Date: 2019-02-12
SPH XING LING SCI & TECH PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, Ginkgo biloba extract also contains ginkgolic acid, a toxic component, and its content range needs to be further reduced to minimize the harm to patients. Moreover, the content range of active ingredients in Ginkgo biloba extract in the current applicable standards is too wide, which is not conducive to accurate Therefore, it is necessary to conduct in-depth research on ginkgo ketone esters to solve the problems of existing products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] This embodiment relates to a preparation method of a low-toxic ginkgo ketone ester composition. The steps are: take ginkgo leaves and grind them, heat and reflux with 65-75% ethanol to extract several times, filter, and the filtrate is used for later use, and the dregs are heated with pure water Reflux for 0.5-1 hour, filter, combine the filtrate with the above-mentioned filtrate, concentrate into a thick paste, dissolve with pure water at 70-90°C, let stand to cool, filter, put the filtrate on a macroporous resin column, and use 20% ethanol, Elute with 30% ethanol and 50% ethanol, concentrate 20% ethanol and 30% ethanol eluate until no alcohol smell, put on polyamide column, elute with ethanol; combine 50% ethanol eluate and ethanol eluate, concentrate Until there is no alcohol smell, the concentrated solution is extracted with cyclohexane, the cyclohexane extract is discarded, the concentrated solution is spray-dried and pulverized to obtain the low-toxic ginkgo ketone...

Embodiment 2

[0018] The low-toxic ginkgo ketone ester composition prepared according to the preparation method of Example 1 comprises the following components in terms of weight percentage: total flavonoids, 44-55%; bilobalide (C 15 h 18 o 8 ), greater than 2.6%; rutin, less than 4%; quercetin, less than or equal to 0.4%; total ginkgolic acid, less than or equal to 4ppm; moisture, less than 5%; cyclohexane residue, less than or equal to 0.002%.

[0019] Optionally, the low-toxic ginkgo ketone ester composition also includes the following components: total flavonol glycosides 24-35%; terpenoid lactone and bilobalide (C 15 h 18 o 8 ), ginkgolide A (C 20 h 24 o 9 ), ginkgolide B (C 20 h 24 o 10 ) and ginkgolide C (C 20 h 24 o 11 ) in the total amount of 6-12%.

[0020] In the existing standard, ginkgolic acid is determined by TLC scanning method, which has low sensitivity; in the technical solution of the present invention, ginkgolic acid is determined by liquid chromatography-ma...

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Abstract

The invention relates to a preparation method of a low-toxicity ginkgo ketone ester composition. The method comprises the following steps of taking and crushing ginkgo leaves; performing heating backflow extraction for many times by 65 to 75-percent ethanol; performing filtering to obtain first filter liquid for later use; adding pure water into medicine slag; performing heating backflow for 0.5 to 1 h; performing filtering to obtain second filter liquid; merging the second filter liquid with the first filter liquid; performing concentration to obtain thick paste; performing dissolving the thick paste by pure water of 70 to 90 DEG C; performing still standing cooling and filtering; feeding third filter liquid onto a macroporous resin column; sequentially performing elution by 20-percent ethanol, 30-percent ethanol and 50-percent ethanol; concentrating the 20-percent ethanol and 30-percent ethanol elution liquid till no ethanol odor exists; feeding the materials into a polyamide column;performing elution by ethanol; merging 50-percent ethanol elution liquid and ethanol elution liquid; performing concentration till no ethanol odor exists; extracting concentration liquid by cyclohexane; abandoning cyclohexane extraction liquid; performing spraying drying on concentrated liquid; performing crushing to obtain the low-toxicity ginkgo ketone ester composition. The low-toxicity ginkgoketone ester composition prepared by the method has the advantages that the toxic ingredient content is further reduced; the solvent residue content is low; the active ingredient content is stable; the medication safety is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and mainly relates to a preparation method of a low-toxic ginkgo ketone ester composition. Background technique [0002] Ginkgo biloba is a kind of medicine with exact curative effect and wide application. The main effective components of its extract are flavonoids (Flavonoids) and terpene lactones. The former is mainly quercetin, kaempferol, isorhamnetin and its glycosides. The latter is mainly based on Ginkgolides and Bilobalide. Clinical verification of ginkgo biloba extract is quite satisfactory for the curative effect of heart, cerebrovascular and peripheral vascular circulation disorders. At present, it is mainly used for the treatment of ischemic cerebrovascular disease and the dizziness caused by it, hearing impairment, senile dementia, diabetic neuropathy, cor pulmonale and epilepsy, such as ginkgo ketone ester raw materials and its preparations. [0003] However, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P25/00A61P1/08A61P27/16A61P25/28A61P3/10A61P11/00A61P9/00A61P25/08A61K31/365A61K31/7048A61K31/352
CPCA61K31/352A61K31/365A61K31/7048A61K36/16A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P1/08A61P3/10A61P9/00A61P11/00A61P25/00A61P25/08A61P25/28A61P27/16A61K2300/00
Inventor 王军高崎沈琴王挚豪高勇张曙平
Owner SPH XING LING SCI & TECH PHARM CO LTD
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