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Saccharomyces cerevisiae strain and applications

A technology of Saccharomyces cerevisiae and a production method, which is applied in the field of microorganisms, can solve problems such as high cost and complicated process of glucoamylase, and achieve the effects of reducing production cost, simplifying fermentation process, and good fermentation performance

Active Publication Date: 2019-01-22
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to address the defects of the prior art, to provide a strain of Saccharomyces cerevisiae with both glucoamylase function and good fermentation performance to solve the problem of saccharification first and then fermentation and additional addition in the bioethanol industry using starch as raw material The complex process and high cost of glucoamylase

Method used

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  • Saccharomyces cerevisiae strain and applications
  • Saccharomyces cerevisiae strain and applications
  • Saccharomyces cerevisiae strain and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Mutagenesis of Saccharomyces cerevisiae

[0045] 1. Dilute the fermentation liquid of Saccharomyces cerevisiae fermented by COFCO Biochemical Energy (Zhaodong) Co., Ltd. 7 , take 100 μ l coating YPD (2% glucose, 2% peptone, 1% yeast powder, 2% agar powder, pH7.0) solid plate, after a single colony grows, pick a single colony and inoculate it into YPD (2% glucose , 2% peptone, 1% yeast powder, pH 7.0) liquid culture medium, 30 ℃, 250rpm carry out shaking flask culture, cultivate to OD600 is about 1.0~1.5, take a certain amount of culture medium and wash the bacteria with sterile physiological saline and use Prepare bacterial suspension with sterile saline.

[0046] 2. Set up a multifunction plasma mutagenesis system (multifunction plasma mutagenesis system, MPMS), using nitrogen as the working gas of the plasma, with a power supply of 300W, a platform height of 14mm, a plasma temperature of 10%. Flow rate 12slpm, under the condition of mutagenesis mode A, dr...

Embodiment 2

[0048] Example 2: Continuous domestication and breeding of ethanol fermentation industrial materials

[0049] Selection Example 1 Screening of Saccharomyces cerevisiae exhibiting glucoamylase activity, continuous domestication of ethanol fermentation industrial materials to ensure the selection of Saccharomyces cerevisiae with better fermentation performance. Continuous domestication was carried out in a 2L fully automatic fermenter; the liquefied mash and distiller's mash from the Phase III (or Phase II) production plant of COFCO Biochemical Energy (Zhaodong) Co., Ltd. were taken to the automated fermenter, and the above-mentioned Saccharomyces cerevisiae was inoculated according to the inoculation ratio of 20%. After the initial fermentation to 36 hours, a certain proportion of fermented mash is taken out, and fresh liquefied mash is added to continue the fermentation, and the operation is repeated for several rounds. After each round of acclimatization, take part of the fer...

Embodiment 3

[0054] Example 3: Saccharomyces cerevisiae COTY real bioethanol fermentation material and pilot test inspection of fermentation process

[0055] The Saccharomyces cerevisiae COTY and the Saccharomyces cerevisiae strains that were domesticated and screened in Example 2 were used for the verification of reducing the use of exogenous glucoamylase in the fermentation process. The two strains were passed through YPD (2% glucose, 2% peptone, 1% Yeast powder, pH 7.0) liquid culture medium to the same cell density, centrifuged to collect bacteria, washed and inoculated to reduce 0%, 15%, 30%, 50%, 75% of exogenous glucoamylase In 200ml of liquefied mash, vibrate at 30°C and 250rpm for 96 hours. After the fermentation, HPLC was used to detect the content of ethanol, residual glucose and glycerin. The results are shown in Table 2.

[0056] Table 2 Detection of ethanol content, residual glucose and glycerin content

[0057]

[0058]

[0059] Note: A: The glucoamylase of the starti...

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Abstract

The invention belongs to the field of microbiology, relates to a saccharomyces cerevisiae strain and applications, and in particular relates to a saccharomyces cerevisiae strain with the efficient saccharifying enzyme function and good fermenting property, and applications of the saccharomyces cerevisiae strain in producing bioethanol. The saccharomyces cerevisiae with the accession number of CGMCC No.13506 has the saccharifying enzyme activity and good fermenting property, and has the enzyme activities of various enzymes relevant to amylolysis, in the fermentation industry of bioethanol, thecoupling of starch saccharification with saccharomyces cerevisiae growth and alcohol fermentation is realized, the adding of 30-50 % of exogenous saccharifying enzyme can be effectively replaced, thusthe fermentation process is simplified, the production cost of alcohol is reduced, and the large-scale industrial production of alcohol is satisfied.

Description

technical field [0001] The invention belongs to the field of microorganisms, and the invention relates to a strain of Saccharomyces cerevisiae and its application, in particular to a strain of Saccharomyces cerevisiae with both high-efficiency glucoamylase function and good fermentation performance and its application for producing bioethanol. Background technique [0002] Due to the excessive dependence on oil, the energy crisis and environmental pollution have become the main factors restricting the economic development of various countries and affecting human health. The development of new energy has become an urgent task. Bioethanol is a kind of green renewable energy, which is mainly obtained from the fermentation of cellulose and starch. It has great production potential and has become a hot spot in energy research and development in the world. [0003] In the bioethanol production industry, starch-rich wheat and corn are mainly used as raw materials, and because of it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/06C12R1/865
CPCC12P7/06C12R2001/865C12N1/185Y02E50/10
Inventor 佟毅李义王小艳张子剑袁敬伟杨勇孟凡铁刘辉吴延东李凡
Owner JILIN COFCO BIOCHEM
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