A strain of Saccharomyces cerevisiae and its application

A technology of Saccharomyces cerevisiae and production method, applied in the field of microorganisms, can solve the problems of complex process and high cost of saccharifying enzyme

Active Publication Date: 2022-03-11
JILIN COFCO BIOCHEM +2
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to address the defects of the prior art, to provide a strain of Saccharomyces cerevisiae with both glucoamylase function and good fermentation performance to solve the problem of saccharification first and then fermentation and additional addition in the bioethanol industry using starch as raw material The complex process and high cost of glucoamylase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Saccharomyces cerevisiae and its application
  • A strain of Saccharomyces cerevisiae and its application
  • A strain of Saccharomyces cerevisiae and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Mutagenesis of Saccharomyces cerevisiae

[0045] 1. Dilute the fermentation liquid of Saccharomyces cerevisiae fermented by COFCO Biochemical Energy (Zhaodong) Co., Ltd. 7 , take 100 μ l coating YPD (2% glucose, 2% peptone, 1% yeast powder, 2% agar powder, pH7.0) solid plate, after a single colony grows, pick a single colony and inoculate it into YPD (2% glucose , 2% peptone, 1% yeast powder, pH 7.0) liquid culture medium, 30 ℃, 250rpm carry out shaking flask culture, cultivate to OD600 is about 1.0~1.5, take a certain amount of culture medium and wash the bacteria with sterile physiological saline and use Prepare bacterial suspension with sterile saline.

[0046] 2. Set up a multifunction plasma mutagenesis system (multifunctionplasma mutagenesis system, MPMS), using nitrogen as the working gas of the plasma, with a power supply of 300W, a platform height of 14mm, a plasma temperature of 10%, and a gas flow rate of 12 slpm, under the condition of mutagenes...

Embodiment 2

[0048] Example 2: Continuous domestication and breeding of ethanol fermentation industrial materials

[0049] Selection Example 1 Screening of Saccharomyces cerevisiae exhibiting glucoamylase activity, continuous domestication of ethanol fermentation industrial materials to ensure the selection of Saccharomyces cerevisiae with better fermentation performance. Continuous domestication was carried out in a 2L fully automatic fermenter; the liquefied mash and distiller's mash from the Phase III (or Phase II) production plant of COFCO Biochemical Energy (Zhaodong) Co., Ltd. were taken to the automated fermenter, and the above-mentioned Saccharomyces cerevisiae was inoculated according to the inoculation ratio of 20%. After the initial fermentation to 36 hours, a certain proportion of fermented mash is taken out, and fresh liquefied mash is added to continue the fermentation, and the operation is repeated for several rounds. After each round of acclimatization, take part of the fer...

Embodiment 3

[0054] Example 3: Saccharomyces cerevisiae COTY real bioethanol fermentation material and pilot test inspection of fermentation process

[0055] The Saccharomyces cerevisiae COTY and the Saccharomyces cerevisiae strains that were domesticated and screened in Example 2 were used for the verification of reducing the use of exogenous glucoamylase in the fermentation process. The two strains were passed through YPD (2% glucose, 2% peptone, 1% Yeast powder, pH 7.0) liquid culture medium to the same cell density, centrifuged to collect bacteria, washed and inoculated to reduce 0%, 15%, 30%, 50%, 75% of exogenous glucoamylase In 200ml of liquefied mash, vibrate at 30°C and 250rpm for 96 hours. After the fermentation, HPLC was used to detect the content of ethanol, residual glucose and glycerin. The results are shown in Table 2.

[0056] Table 2 Detection of ethanol content, residual glucose, and glycerin content

[0057] g / 100ml A A15 A30 A50 A75 B15 B30 B50 B75 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of microorganisms, and the invention relates to a strain of Saccharomyces cerevisiae and its application, in particular to a strain of Saccharomyces cerevisiae with both high-efficiency glucoamylase function and good fermentation performance and its application for producing bioethanol. The Saccharomyces cerevisiae with the preservation number of CGMCC No.13506 in the present invention has saccharification enzyme activity and good fermentation performance, and exhibits various enzyme activities related to starch hydrolysis. In the bioethanol fermentation industry, starch saccharification and Saccharomyces cerevisiae The coupling of growth and ethanol fermentation can effectively replace the addition of 30-50% exogenous glucoamylase, simplify the fermentation process, reduce the cost of ethanol production, and meet the large-scale industrial production of ethanol.

Description

technical field [0001] The invention belongs to the field of microorganisms, and the invention relates to a strain of Saccharomyces cerevisiae and its application, in particular to a strain of Saccharomyces cerevisiae with both high-efficiency glucoamylase function and good fermentation performance and its application for producing bioethanol. Background technique [0002] Due to the excessive dependence on oil, the energy crisis and environmental pollution have become the main factors restricting the economic development of various countries and affecting human health. The development of new energy has become an urgent task. Bioethanol is a kind of green renewable energy, which is mainly obtained from the fermentation of cellulose and starch. It has great production potential and has become a hot spot in energy research and development in the world. [0003] In the bioethanol production industry, starch-rich wheat and corn are mainly used as raw materials, and because of it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/06C12R1/865
CPCC12P7/06C12R2001/865C12N1/185Y02E50/10
Inventor 佟毅李义王小艳张子剑袁敬伟杨勇孟凡铁刘辉吴延东李凡
Owner JILIN COFCO BIOCHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap