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A method for preparing antioxidant peptide from pig skin by enzymatic hydrolysis

A technology of antioxidant peptides and pigskin, applied in the direction of fermentation, etc., can solve the problems of pigskin backlog, low digestibility, and insufficient nutritional value and function of pigskin, etc., achieve good antioxidant activity and simple preparation process Effect

Inactive Publication Date: 2019-01-18
JIANGSU CHANGSHOU GRP CO LTD
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the most important applications of animal skin is tanning, but due to the poor texture of pig skin itself, pig skin is rarely used in the leather industry at present. In addition, because pig skin has unacceptable peculiar smell, bad taste, Because of low digestibility and inconvenient processing, it is often discarded and rarely used directly, resulting in a large backlog of pigskin, and the nutritional value and function of pigskin have not been fully utilized.

Method used

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Examples

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Embodiment

[0018] This example discloses a method for enzymatically hydrolyzing pigskin to prepare antioxidant peptides, comprising the following steps:

[0019] 1) Use pigskin as raw material, cut off the surface grease, cut into 1-2cm wide strips, use a high-speed tissue grinder to beat the pigskin into a homogenate, gradually add 2 times the volume of drinking water during the grinding process, and beat for later use;

[0020] 2) Place the homogenate in step 1) in an enzymatic hydrolysis tank for enzymatic degreasing. The enzymatic degreasing conditions are as follows: add 1-1.5% (w / v) lipase, adjust the pH to 5, and perform enzymatic hydrolysis at 45°C for 4 hours; The degreasing centrifugation conditions are: at room temperature, the rotation speed is 5000r / min, and the time is 15min;

[0021] 3) The aqueous phase and solid phase obtained in step 2), i.e. the pigskin protein rich product, the composite protease is flavor protease, alkaline protease and papain, and the flavor proteas...

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PUM

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Abstract

The invention discloses a method for preparing antioxidant peptide by enzymatic hydrolysis of pig skin, which comprises the following steps: 1) taking pig skin as raw material, cutting off surface oiland fat, and cutting into 1-2cm wide strips, beatening for standby; 2) placing the homogenate of the step 1) in an enzymatic hydrolysis tank for enzymatic hydrolysis defatting; 3) adding 1 volume ofdrinking water into the aqueous phase and the solid phase obtained in the step 2), adjusting the pH to 7.3, and then adding 2% of the pigskin protein rich substance; 3% complex proteinase (flavor proteinase: alkaline proteinase: papain = 1: 3: 2), the temperature is 50 DEG C, the hydrolysis time is 8h, the enzyme is inactivate, and the pigskin antioxidant peptide is obtained by centrifugation, ultrafiltration, concentrate and drying the powder in turn. The invention has the beneficial effects that the antioxidant peptide is prepared from pig skin by the enzymatic hydrolysis method, green environmental protection and high-value utilization of pork processing by-products are realized, the preparation process is simple, and the prepared peptide powder has good antioxidant activity.

Description

technical field [0001] The invention relates to the technical field of preparation of antioxidant peptides, in particular to a method for enzymolyzing pigskin to prepare antioxidant peptides. Background technique [0002] my country is a big pig raising country. With the continuous development of science and technology, the pig industry has also developed rapidly. The output of pork accounts for about 70% of the total meat output in my country. A large number of by-products are produced while producing pork, and pig skin is one of them. One of the most important applications of animal skin is tanning, but due to the poor texture of pig skin itself, pig skin is rarely used in the leather industry at present. In addition, because pig skin has unacceptable peculiar smell, bad taste, Because of low digestibility and inconvenient processing, it is often discarded and rarely used directly, resulting in a large backlog of pigskin, and the nutritional value and function of pigskin ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 席军付才力
Owner JIANGSU CHANGSHOU GRP CO LTD
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