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Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof

A technology of Pseudomonas aeruginosa and Pseudomonas aeruginosa, applied in the field of microorganisms, can solve problems such as pesticide residues, environmental pollution, drug-resistant pathogens, etc.

Active Publication Date: 2019-01-18
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control of soil-borne diseases in the production of tobacco fields is mainly based on chemical control. The long-term use of pesticides is likely to cause problems such as environmental pollution, pesticide residues, and drug-resistant pathogens.

Method used

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  • Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof
  • Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof
  • Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the separation of soil microorganism

[0031] Collect tobacco rhizosphere soil in the tobacco field of Zhongzhuang Village, Fenchen Township, Xiangcheng County, Henan Province, weigh 1g of soil, add 10ml of sterile water to fully shake to obtain a soil suspension, and then shake the soil suspension to make appropriate dilution (can be carried out) Doubling dilution), take 0.1ml of the diluted solution and spread it on the KB solid medium containing 8-hydroxyquinoline (0.05g / L), and culture it upside down in a constant temperature incubator at 28°C for 36h until colonies grow out, pick Colonies with different sizes, colors or shapes were separated and purified by streaking on the surface of KB solid medium, and 14 strains were obtained, which were named PA2101, PA210302, PA210401, PA210402, PA2108, PA210901, PA210902, PA2112, PA2113, PA2115, PA211601, PA2117, PA2118, and PA2120.

Embodiment 2

[0032] Embodiment 2, the screening of biocontrol bacteria

[0033] Use the plate confrontation method to screen biocontrol bacteria: inoculate Phytophthora phytophthora and root black rot pathogen Phytophthora rhizoctonia on PDA medium, culture at 25°C and 28°C for 5-10 days, and sterilize The puncher punches the pathogenic bacteria colony into a circular hyphae block with a diameter of 5 mm. Inoculate the pathogenic bacteria block (Monococcus nicotianae and Phytophthora nicotianae) and the isolated bacteria on the PDA medium, the distance between the pathogenic bacteria block and the isolated bacteria is 20mm, and set the pathogenic bacteria block alone as a control. Each treatment was repeated 3 times, and the results of standoff culture were checked after 1 week. Among them, only PA2101 has the highest bacteriostatic rate against the two strains of pathogenic bacteria (see Table 1).

[0034] Table 1 The antibacterial results of 14 strains isolated from the soil against Ph...

Embodiment 3

[0040] Embodiment 3, identification of biocontrol bacteria

[0041]Strain morphology, physiology and biochemistry: strain PA2101 is a Gram-negative bacterium, cultured on KB medium at 28°C for 48 hours, the shape of the colony is yellow and flat, the surface is slightly smooth and nearly round, the center of the circle is slightly convex, and the color is heavier than the surrounding , the edge is irregular, the colony is translucent, surrounded by a yellow-green diffusible fluorescent substance that is visible to the naked eye, and the color of the colony becomes darker and darker after a few days ( figure 2 ). It cannot grow at 4°C, but can grow at 41°C. Oxidase, arginine dihydrolase, gelatin liquefaction and nitrate reduction tests were positive, starch could not be hydrolyzed, and denitrification test, methyl red and VP tests were all negative.

[0042] Sequencing: the bacterial strain PA2101 was sequenced, and the identification result was Pseudomonas aeruginosa.

[0...

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Abstract

The invention discloses a Pseudomonas aeruginosa strain capable of simultaneously controlling tobacco black shank and root black rot and having growth promoting effect and application thereof. The Pseudomonas aeruginosa is PA2101, Deposited at China Typical Culture Collection Center, Luojia Mountain, Wuchang, Wuhan, China, Deposited No.: CCTCC NO: M 2018546, Deposited on August 17, 2018. A biocontrol bacterium PA2101 capable of simultaneously controlling tobacco black shank and root black rot is firstly screened from tobacco field soil of Zhongzhuang Village, Fenchen Township, Xiangcheng County, Henan Province. The strain is identified as Pseudomonas aeruginosa, and has the function of promoting tobacco seedling growth. The experiment shows that the Pseudomonas aeruginosa has good inhibition effect on the pathogens of tobacco black shank and root black rot, and the control effect of the Pseudomonas aeruginosa on the tobacco black shank and root black rot can reach above 62%.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa capable of simultaneously preventing and treating tobacco black shank and root black rot and having a growth-promoting effect, a screening method and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] Tobacco is one of the main economic crops in my country and an important source of income in tobacco-growing areas. It has been found that it has great medicinal and health value, and can be used as the best model material for biological science. However, tobacco diseases restrict the healthy growth of tobacco, especially the occurrence of soil-borne diseases such as tobacco black shank and tobacco root black rot will cause tobacco production reduction, and when the disease is serious, it will cause devastating losses. [0003] Tobacco black shank is one of the most serious soil-borne diseases in tobacco production, and the pathogen is Phytop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/02A01P3/00A01P21/00C12R1/385
CPCA01N63/10C12N1/02C12N1/20C12N1/205C12R2001/385
Inventor 文艺千慧敏赵辉高素霞刘新涛李淑君李成军邱睿倪云霞刘红彦
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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