Production of Vitamin B by Fermentation with Bacillus 2 culture medium and method
A fermentation medium and Bacillus technology, applied in the field of biological fermentation, can solve the problems of difficult biological fermentation metabolism control, high dissolved oxygen requirements, slow utilization rate, etc. fast effect
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Embodiment 1
[0020] 1. Seed preparation: Inoculate the activated Bacillus into the sterilized seed medium for seed cultivation, the seed inoculation amount is 1.5%.
[0021] Seed medium composition: 1.5% sucrose, 0.2% glucose, 0.5% corn steep liquor, 0.1% ammonium sulfate, 0.1% light calcium carbonate, 0.1% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 13 minutes .
[0022] Seed culture conditions: culture temperature 34°C, ventilation rate 1: 0.4v / v / min with stirring, culture for 16hr to obtain first-grade seed liquid.
[0023] 2. Fermentation: Inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 5%.
[0024] Composition of fermentation medium: 3.5% sucrose, 1.5% glucose, 0.5% urea, 3.0% corn steep liquor, 0.2% ammonium sulfate, 0.01% magnesium sulfate, 0.1% light calcium carbonate, 0.1% dipotassium hydrogen phosphate, prepared by weight volume ratio , and keep the p...
Embodiment 2
[0030] 1. Seed preparation: Inoculate the activated Bacillus into the sterilized seed medium for seed cultivation, the seed inoculation amount is 5%.
[0031] Seed medium composition: 4.0% sucrose, 0.5% glucose, 2.5% corn steep liquor, 0.3% ammonium sulfate, 0.5% light calcium carbonate, 0.5% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 16 minutes .
[0032] Seed culture conditions: culture temperature 39°C, ventilation rate 1: 0.7v / v / min with stirring, cultivate for 20hrs to obtain seed liquid.
[0033] 2. Fermentation: inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 15%.
[0034] Composition of fermentation medium: 5.0% sucrose, 0.5% glucose, 1.5% urea, 1.5% corn steep liquor, 1.0% ammonium sulfate, 0.15% magnesium sulfate, 0.5% light calcium carbonate, 0.5% dipotassium hydrogen phosphate, prepared by weight volume ratio , and hold pressure at 118...
Embodiment 3
[0041] 1. Seed preparation: Inoculate the activated seeds into the sterilized seed medium for seed cultivation, the seed inoculation amount is 3%.
[0042] Seed medium composition: 2.5% sucrose, 0.25% glucose, 1.5% corn steep liquor, 0.2% ammonium sulfate, 0.25% light calcium carbonate, 0.25% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 15 minutes .
[0043] Seed culture conditions: culture temperature 37°C, ventilation rate 1: 0.6v / v / min with stirring, cultivate for 19hr to obtain seed liquid.
[0044] 2. Fermentation: inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 10%.
[0045] Composition of fermentation medium: 4.0% sucrose, 1.0% glucose, 1.0% urea, 2.5% corn steep liquor, 0.6% ammonium sulfate, 0.12% magnesium sulfate, 0.25% light calcium carbonate, 0.25% dipotassium hydrogen phosphate, prepared by weight volume ratio , and keep the pressure at ...
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