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Production of Vitamin B by Fermentation with Bacillus 2 culture medium and method

A fermentation medium and Bacillus technology, applied in the field of biological fermentation, can solve the problems of difficult biological fermentation metabolism control, high dissolved oxygen requirements, slow utilization rate, etc. fast effect

Active Publication Date: 2021-12-07
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the fermentation process, seed cultivation and fermentation have higher requirements for dissolved oxygen, and a large amount of molasses is used at the same time, because the molasses gathers all the non-sugar substances that cannot be removed and the insoluble sugar added during the sugar production process, as well as various degradation products and nascent The coloring substances are complicated in composition, and are wasteful in microbial fermentation, and it is difficult to control the metabolism of biological fermentation. Nitrogen sources are mostly late-acting organic nitrogen sources such as yeast powder, which are slow in utilization and high in residues.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Seed preparation: Inoculate the activated Bacillus into the sterilized seed medium for seed cultivation, the seed inoculation amount is 1.5%.

[0021] Seed medium composition: 1.5% sucrose, 0.2% glucose, 0.5% corn steep liquor, 0.1% ammonium sulfate, 0.1% light calcium carbonate, 0.1% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 13 minutes .

[0022] Seed culture conditions: culture temperature 34°C, ventilation rate 1: 0.4v / v / min with stirring, culture for 16hr to obtain first-grade seed liquid.

[0023] 2. Fermentation: Inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 5%.

[0024] Composition of fermentation medium: 3.5% sucrose, 1.5% glucose, 0.5% urea, 3.0% corn steep liquor, 0.2% ammonium sulfate, 0.01% magnesium sulfate, 0.1% light calcium carbonate, 0.1% dipotassium hydrogen phosphate, prepared by weight volume ratio , and keep the p...

Embodiment 2

[0030] 1. Seed preparation: Inoculate the activated Bacillus into the sterilized seed medium for seed cultivation, the seed inoculation amount is 5%.

[0031] Seed medium composition: 4.0% sucrose, 0.5% glucose, 2.5% corn steep liquor, 0.3% ammonium sulfate, 0.5% light calcium carbonate, 0.5% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 16 minutes .

[0032] Seed culture conditions: culture temperature 39°C, ventilation rate 1: 0.7v / v / min with stirring, cultivate for 20hrs to obtain seed liquid.

[0033] 2. Fermentation: inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 15%.

[0034] Composition of fermentation medium: 5.0% sucrose, 0.5% glucose, 1.5% urea, 1.5% corn steep liquor, 1.0% ammonium sulfate, 0.15% magnesium sulfate, 0.5% light calcium carbonate, 0.5% dipotassium hydrogen phosphate, prepared by weight volume ratio , and hold pressure at 118...

Embodiment 3

[0041] 1. Seed preparation: Inoculate the activated seeds into the sterilized seed medium for seed cultivation, the seed inoculation amount is 3%.

[0042] Seed medium composition: 2.5% sucrose, 0.25% glucose, 1.5% corn steep liquor, 0.2% ammonium sulfate, 0.25% light calcium carbonate, 0.25% magnesium sulfate, prepared according to weight-volume ratio, and kept at 118±2°C for 15 minutes .

[0043] Seed culture conditions: culture temperature 37°C, ventilation rate 1: 0.6v / v / min with stirring, cultivate for 19hr to obtain seed liquid.

[0044] 2. Fermentation: inoculate the above-mentioned seed liquid into the fermentation medium for fermentation and cultivation, and the inoculation amount is 10%.

[0045] Composition of fermentation medium: 4.0% sucrose, 1.0% glucose, 1.0% urea, 2.5% corn steep liquor, 0.6% ammonium sulfate, 0.12% magnesium sulfate, 0.25% light calcium carbonate, 0.25% dipotassium hydrogen phosphate, prepared by weight volume ratio , and keep the pressure at ...

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PUM

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Abstract

The invention relates to a method for producing vitamin B by fermenting bacillus 2 The culture medium is the culture method. The present invention uses corn steep liquor, urea, sucrose and other medium-effect carbon-nitrogen sources between slow-acting carbon-nitrogen sources and quick-acting carbon-nitrogen sources as fermentation base materials to remove the repression effect of quick-acting carbon source glucose. , while eliminating the use of molasses and yeast powder, making vitamin B 2 Almost all of the fermentation basic materials are soluble materials with very little solid content and fast utilization speed to achieve full utilization effect, thereby reducing the residual raw materials in the fermentation liquid in the tank and the cost of fermentation raw materials, and improving the dissolved oxygen level in the fermentation process. Increased fermentation level.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular to a method for producing vitamin B by fermentation of Bacillus spores 2 Culture medium and cultivation method. Background technique [0002] Vitamin B 2 (chemical formula: C 17 h 20 N 4 o 6 , formula weight 376.37) also known as riboflavin, slightly soluble in water, stable when heated in neutral or acidic solution. Vitamin B 2 It is a part of the prosthetic group of yellow enzymes in the body (yellow enzyme plays the role of hydrogen transfer in biological redox). When it is lacking, it will affect the biological oxidation of the body and cause metabolic disorders. Vitamin B 2 The two properties of the main cause of its loss: (1) can be destroyed by light; (2) can be destroyed by heating in alkaline solution. Vitamin B 2 It can be applied in medicine, food, feed and other industries, and the development space and market demand are unlimited. [0003] Vit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P25/00C12R1/07
CPCC12P25/00
Inventor 郭海燕李学兵孙瑞君张志胡晓玲盛雪胡钦龙冯占梅
Owner NINGXIA QIYUAN PHARMA
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