Skin cream with effects of removing acnes, eliminating acne marks and whitening and preparation method of skin cream
A skin cream and anti-acne technology, which is applied in skin care preparations, skin diseases, and pharmaceutical formulas, can solve the problems of destroying the normal flora of the skin, difficult to completely remove marks, and inability to fundamentally remove acne, etc., achieving remarkable results. Free radical scavenging ability, enhance the anti-acne effect, and help smooth the effect of acne pits
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Embodiment 1
[0035] Example 1 Preparation of Anji White Tea Callus Culture Filtrate
[0036] The filtrate of Anji white tea callus culture is prepared by the following steps:
[0037] (1) Take the leaves of Anji white tea as explants for inducing callus, soak them in 70% ethanol solution for 5 minutes on an ultra-clean workbench, then rinse them with sterile water for 3 to 5 times, and absorb them with sterile filter paper. Dry surface water, cut into small pieces of 0.3cm×0.3cm, and inoculate them in a solid medium of MS+2mg / L TDZ+4g / L agar for callus induction. The culture condition is 27℃ and light time 12h / d. Light intensity 30mmoL·m -2 ·S -1 ; The training period is 45 days;
[0038] (2) Take the callus with good growth obtained in step (1), divide it into mung bean size with sterilized tweezers on an ultra-clean workbench, and inoculate it to 40mL MS+2mg / L TDZ+2mg / L IAA, a 100mL Erlenmeyer flask of liquid culture medium with pH 5.8, on a constant temperature shaker at 27°C, 100rpm, ligh...
Embodiment 2
[0039] Example 2 Preparation of plant fruit seed superfine powder
[0040] Example 2 The formula composition of the plant fruit seed superfine powder is: based on 100g plant fruit seed superfine powder, it includes the components shown in the following table:
[0041] Component
[0042] Preparation method: mix and pulverize the moringa seeds, wild grape seeds and buckwheat seeds of the formula to obtain a mixed powder of seeds; add the mixed powder of seeds to a jet mill and perform jet milling. The fully ground powder is followed by The airflow passes through the gap of the sorting wheel, but the ones that are not sufficiently ground cannot pass. The airflow mill is continued until the particle size of the powder is 0.2-1μm, and the superfine plant fruit seed powder is collected.
Embodiment 3
[0043] Example 3 Preparation of growth factor-containing liposomes
[0044] Example 3 The formulation composition of the growth factor-containing liposomes, in parts by weight, contains the components described in Table 1 below:
[0045] Table 1 Formulation composition of liposomes containing growth factors
[0046]
[0047] Formulation 1 The preparation of growth factor-containing liposomes includes the following steps:
[0048] (1) Weigh the formula amount of vitexin, hydrogenated soybean lecithin, cholesterol, β-sitosterol and Tween-80, mix, add absolute ethanol, fully stir to dissolve to obtain solution A;
[0049] (2) Dissolve the recombinant human keratinocyte growth factor in a PBS buffer solution with a pH of 7.2, stir until it is completely dissolved to make a 0.1-0.2 mg / mL solution, and then add the formula amount of Anji white tea callus culture filtrate, Mix well to obtain solution B;
[0050] (3) Under continuous stirring with a magnetic stirrer, use a syringe to inject sol...
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