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A kind of Streptomyces alboflavinum and the method and application of using the microorganism to prepare biocontrol microbial agent

A biocontrol technology for microorganisms and Streptomyces alboflavinus, which is applied in the field of agricultural microorganisms and achieves good application prospects, short fermentation and culture period, and simple preparation process

Active Publication Date: 2021-10-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biological control of Ralstonia solanacearum mainly focuses on the use of bacteria such as Bacillus and Pseudomonas, and there are few reports on the use of actinomycetes, especially Streptomyces, to inhibit Ralstonia solanacearum.

Method used

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  • A kind of Streptomyces alboflavinum and the method and application of using the microorganism to prepare biocontrol microbial agent
  • A kind of Streptomyces alboflavinum and the method and application of using the microorganism to prepare biocontrol microbial agent
  • A kind of Streptomyces alboflavinum and the method and application of using the microorganism to prepare biocontrol microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0046] (1) Preparation of culture medium

[0047] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0048] Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; tap water 1000mL, pH7.2.

[0049] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL. The concentration of other inorganic salts with different concentrations is adjusted on this basis.

[0050] Fermentation medium: Low-value grains soaked in tap water for 6-8 hours (grai...

Embodiment 2

[0064] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0065] (1) Preparation of culture medium

[0066] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0067] Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; tap water 1000mL, pH7.2.

[0068] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL.

[0069] Fermentation medium: Low-value grains soaked in tap water for 6-8 hours (grains such as old rice, old corn, old sorghum, and old wheat that have been stored for more than one yea...

Embodiment 3

[0083] A preparation method of biocontrol microbial bacterial agent, the steps are as follows:

[0084] (1) Preparation of culture medium

[0085] Preservation medium: potassium nitrate, 1g; soluble starch, 20g; soybean powder, 10g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; agar, 20g ; Tap water 1000mL, pH7.2.

[0086]Seed medium: glucose, 65g; soybean flour, 10g; potassium nitrate, 0.3g; magnesium sulfate, 1g; dipotassium hydrogen phosphate, 1g; sodium chloride, 0.5g; potassium chloride, 0.2g; g; Tap water 1000mL, pH7.2.

[0087] Inorganic salt concentration C preparation: potassium nitrate, 1g; dipotassium hydrogen phosphate, 0.5g; sodium chloride, 0.5g; magnesium sulfate, 0.5g; ferrous sulfate, 0.01g; tap water 1000mL.

[0088] Fermentation medium: low-value grains soaked in tap water for 6-8 hours (grains such as old rice, old corn, old sorghum, and old wheat that have been stored for more than a year),...

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Abstract

The invention relates to a Streptomyces alboflavinum and a method and application of using the microorganism to prepare a biocontrol microbial agent. The preparation method comprises the following steps: placing the Streptomyces alboflavinus in a preservation medium, activating it at 28-32°C for 5-7 days, and then placing it in seeds culture medium at 30°C and 160-180r / min for 36-48 hours to obtain seed bacterial liquid; inoculate the seed bacterial liquid into the fermentation medium, ferment and cultivate in a constant temperature incubator at 30°C for 7 days, and then ferment and cultivate A good substrate is placed in a ventilated oven at 25-35°C and / or an air source heat pump dryer to dry or naturally dried at room temperature, and then pulverized to obtain a biocontrol microbial agent. The biocontrol microbial bacterial agent prepared by the method can be applied in antagonism to R. solanacearum, can be applied in salt tolerance, drought tolerance, and dissolution of soil inorganic phosphorus, and can be applied in synthesizing siderophores and growth hormone indole acetic acid, It can also be used to promote the germination and growth of tomato seeds in plants.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, in particular to Streptomyces alboflavinum and a method and application of using the microorganism to prepare a biocontrol microbial agent. Background technique [0002] With the improvement of people's living standards, especially people's requirements for improved quality of life, the world is actively developing green and organic ecological agriculture. In the process, it is required to use no or limited use of chemical fertilizers, chemical pesticides and other chemical substances, etc. . However, at present, most of the prevention and control of plant diseases still rely on chemical agents for prevention and control, which has an impact on the ecological environment. The resistance of pathogenic microorganisms is increasing, and it also seriously affects the quality of agricultural products and the health of the people, which is not conducive to national health and our co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P1/00A01P21/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 王昌禄薛意斌路来风李贞景刘春静武淑芬陈勉华郭庆彬刘欢欢杨明冠李风娟
Owner TIANJIN UNIV OF SCI & TECH
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