Use of mussel polysaccharides and degradation products thereof

A technology of degradation products, mussel polysaccharides, applied in the fields of application, polysaccharide/gum-containing food ingredients, medical preparations containing active ingredients, etc., to achieve the effect of obvious prebiotic effect and easy availability of raw materials

Active Publication Date: 2018-12-28
SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent (CN 101357952 A) discloses the application of mussel polysaccharides in the preparation of blood lipid-lowering drugs or food; Chinese patent (CN 101991038 A) discloses the effects of mussel polysaccharides in lowering cholesterol, anti-radiation and eliminating heavy

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  • Use of mussel polysaccharides and degradation products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of mussel polysaccharide

[0017] Use frozen boiled dried thick-shelled mussels as raw material, pulverize them with a pulverizer, and pass through a 20-mesh sieve to obtain dried mussel powder; put the dried mussel powder into an extraction tank, add 6 times the volume of water, extract at 100°C for 2 hours, and stir with a paddle The rotation speed is 60 rpm; use a 50-mesh screen to separate the mussel meat residue and polysaccharide extract; adjust the pH of the prepared polysaccharide extract to 8.0 with NaOH, add alkaline protease with a mass ratio of 0.2%, and enzymolyze at 50°C 2 hours, after the enzymolysis is completed, inactivate at 80°C for 15 minutes to obtain the enzymolysis solution; use diatomaceous earth as a filter aid, filter the enzymolysis solution with a plate frame to obtain a clear polysaccharide solution, the amount of diatomaceous earth It is 2 kg / square meter; Add 1.5 times of ethanol with a volume mass fraction of 93% to ...

Embodiment 2

[0018] Example 2 Preparation of mussel oligosaccharides

[0019] Add water to the mussel polysaccharide in Example 1 to prepare a 10% solution, adjust the pH to 6.0 with HCl to obtain a mussel polysaccharide solution, and then add α-amylase to the 10% mussel polysaccharide aqueous solution at a ratio of 220 U / L medium, incubated at 30°C for 10 h, inactivated at 100°C for 15 min, filtered through a 0.22 μm filter membrane, collected the filtrate, concentrated, and freeze-dried to obtain the crude mussel oligosaccharide extract. Take an appropriate amount of mussel oligosaccharide crude extract, dissolve it in deionized water to a solution of 0.5 g / mL, and use a polyacrylamide gel Bio-Gel P-2 column (2.5 cm×125 cm) for separation to remove Ionized water was used as the eluent at a flow rate of 37.5 mL / h, collected once every 8 min, and the elution curve was drawn using the phenol-sulfuric acid method for follow-up detection. The peak position, the collected solution was concent...

Embodiment 3

[0020] Example 3 Effects of mussel polysaccharides and mussel oligosaccharides on intestinal flora

[0021] 24 male SD rats, weighing 140-160g, were divided into normal group, high-fat diet group, mussel polysaccharide group and mussel oligosaccharide group. After 7 days of quarantine, all rats were randomly divided into 2 groups according to body weight, 6 of which were given maintenance feed as a blank control, and 18 were given high-fat feed. After 2 weeks of high-fat feed, they were randomly divided into three groups according to body weight. Continue to give high-fat feed, one group was given mussel polysaccharide 600 mg / kg / d by gavage, one group was given mussel oligosaccharide 200 mg / kg / d, and the other group was given equal volume of purified water by gavage.

[0022] Among them, the high-fat feed formula was 67.5% maintenance feed, 10% lard, 10% egg yolk powder, 10% sucrose, 2% cholesterol and 0.5% cholate.

[0023] After the 6th week of mussel polysaccharide adminis...

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Abstract

The invention provides use of mussel polysaccharides and degradation products thereof for regulating intestinal microflora, and use of preparation of a food, a health food or a medicine for regulatingintestinal microflora of a human body and feed or a medicine for regulating intestinal microflora of animals. A food, a health food, feed and a medicine containing the mussel polysaccharides and thedegradation products thereof are provided. The mussel polysaccharides have a molecular weight ranging from 2 KDa to 400 KDa. The degradation product is mussel polysaccharides, the molecular weight range is 300 Da to 2 KDa, and the degree of polymerization is 2-10. The mussel polysaccharides serving as a prebiotic raw material are extracted from the marine animal mussel, the source of the raw material is rich, the production and preparation costs are low, the proportion of the phylum firmicutes of obese model rats can be significantly reduced, the proportion of bacteroides is improved, and themussel polysaccharides play a role of improving intestinal microecology and is a novel prebiotic.

Description

technical field [0001] The invention belongs to the application field of natural products, and in particular relates to the use of mussel polysaccharide and its degradation product in regulating intestinal microbial flora. Background technique [0002] With the development of intestinal microbiology research, the importance of the balance of intestinal microbial flora to health has gradually been recognized by people. Studies have shown that at different stages of human development, gut microbes have corresponding characteristics and fully affect physiological functions. During the aging process of the human body, reduced exercise, nutritional changes, changes in drugs and living conditions may all affect microorganisms and their products, resulting in changes in physiological and immune states. The human body's own genome is difficult to change, but the microbiome can be intervened and regulated by various means such as fecal bacteria transplantation, prebiotics / bacteria, ...

Claims

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Application Information

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IPC IPC(8): A23L33/21A23K20/163A61K31/715A61P1/00
CPCA61P1/00A23K20/163A23L33/21A61K31/715A23V2002/00A23V2200/32A23V2250/51A23V2250/284A23V2250/2042
Inventor 邵华荣刘飞吴季栩张金华程艳玲郑晓霞刘纯朱希强凌沛学
Owner SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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