Method for producing kidney bean or lentil mutant by using EMS mutagenesis

A technology of lentils and mutants, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as the inability to simulate the influence of environmental factors, and achieve the effect of improving the survival rate

Active Publication Date: 2018-12-28
CORN RES INST SHANXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, relying solely on the chemical mutagenic factors of the EMS mutagen to mutate kidney beans and lentils cannot simulate the influence of environmental factors

Method used

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  • Method for producing kidney bean or lentil mutant by using EMS mutagenesis
  • Method for producing kidney bean or lentil mutant by using EMS mutagenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0041] S1, the plant seeds were soaked in water for 12 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were kidney bean seeds; the specific steps were as follows:

[0042] Select red kidney bean seeds with consistent color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml of HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0043] S2, germination treatment

[0044]Place the sterile seeds flat on the germination matrix, and make the liquid level of the germination matrix 2 mm lower than the upper surface of the sterile seeds, put them in an artificial climate box to germinate the sterile seeds, and after one young leaf gro...

Embodiment 2

[0052] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0053] S1, the plant seeds were soaked in water for 6 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were kidney bean seeds; the specific steps were as follows:

[0054] Select red kidney bean seeds with consistent color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml of HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0055] S2, germination treatment

[0056] Place the sterile seeds flat on the germination matrix, and make the liquid level of the germination matrix 1 mm lower than the upper surface of the sterile seeds, put them into an artificial climate box to germinate the sterile seeds, and after two young leaves...

Embodiment 3

[0062] A method utilizing EMS mutagenesis to produce kidney beans or lentil mutants, comprising the following steps:

[0063] S1, the plant seeds were soaked in water for 12 hours, and then disinfected after being taken out to obtain sterile seeds; the plant seeds were lentil seeds; the specific steps were as follows:

[0064] Select the lentil seeds with the same color, full grain and no insects, soak them in water for 12 hours, take them out and put them in 0.1g / 100ml HgCl 2 The solution was soaked and disinfected for 6 minutes, then rinsed with sterile water, and the water on the surface of the seeds was blotted dry with filter paper to obtain sterile seeds;

[0065] S2, germination treatment

[0066] Place the aseptic seeds flat in the germination matrix, and make the liquid level of the germination matrix lower than the upper surface of the aseptic seeds, put them into an artificial climate box to germinate the aseptic seeds, and after two young leaves grow, the Germina...

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Abstract

The invention belongs to the technical field of kidney bean breeding, and particularly relates to a method for producing a kidney bean or lentil mutant by using EMS mutagenesis. The method includes seed disinfection treatment, germination treatment, mutagenic treatment and field planting, wherein the germination treatment step includes placing sterile seeds in a germination matrix flatly, and making the germination substrate liquid surface lower than the upper surface of the sterile seeds, so that the sterile seeds germinate, and obtaining germinated plant bodies after 1-2 young leaves are grown; wherein the mutagenic treatment step includes adding an EMS solution to per kilogram of germination substrate, using the EMS solution to mutagenize the germinated plant bodies, taking out the germinated plant bodies, placing the germinated plant bodies in an MS liquid culture medium to perform photoperiod culture for 5-7 days, adding an EMS solution into every kilogram of MS liquid culture medium, and using EMS to continue to mutagenize the germinated plant bodies to obtain the mutagenized plant bodies. The method is an efficient mutagenesis method for red kidney beans, which improves theefficiency of mutagenesis, reduces the amount of carcinogens such as the EMS, and reduces the health threat to breeding workers.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for producing kidney bean or lentil mutants through EMS mutagenesis. Background technique [0002] Mutagenesis is one of the more commonly used methods in plant breeding, including (1) physical mutagenesis, such as radiation, laser microbeam, ion beam, microwave, ultrasonic, heat and other methods to cause heritable variation in plants. It mainly acts on the DNA in plants through these ion beams, causing DNA fragment loss, translocation, inversion, etc.; (2) chemical mutagenesis, which uses chemical mutagens to treat plant materials. There are many types of chemical mutagens, such as alkylating agents, nitroso compounds, azide compounds, base analogs, antibiotics, and light amines. At present, among these chemical mutagens, the most widely researched and applied ones are mainly alkylating agents, mainly including, EMS, NaN3, DES and methyl sub-MNU. T...

Claims

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Application Information

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IPC IPC(8): A01H3/02A01H3/04A01H4/00
CPCA01H3/02A01H3/04A01H4/001
Inventor 郭贵青陈喜明韩云丽
Owner CORN RES INST SHANXI ACAD OF AGRI SCI
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