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hptlc-biological imaging screening method for phenolic antioxidants synthesized from oils and fats

A phenolic antioxidant and biological technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of low sensitivity and specificity, interference, etc.

Active Publication Date: 2020-10-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and specificity of the UV absorption mode are low, and it is very susceptible to interference from food matrix substances.

Method used

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  • hptlc-biological imaging screening method for phenolic antioxidants synthesized from oils and fats
  • hptlc-biological imaging screening method for phenolic antioxidants synthesized from oils and fats
  • hptlc-biological imaging screening method for phenolic antioxidants synthesized from oils and fats

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of BHT and TBHQ standard solutions: use methanol as a solvent, dilute with methanol to prepare a standard solution with a concentration of 0.01mg / mL;

[0033] (2) High-efficiency thin-layer chromatography separation: 2-8ul standard solution and edible oil sample extract are used for non-contact purging spotting with Linomat 5, and after spotting is completed, use developing solution (toluene / ethyl acetate / methanol, 9 / 1 / 1, v / v / v) unfold, unfold upward 60mm, take out the silica gel plate and place it on a flat heater at 50°C for 3 minutes to fully dry;

[0034] (3) Specific bioimaging: Evenly spread the biological developer solution on the unfolded silica gel plate by dipping, take it out and put it into a thin-layer imaging system, and place it under the irradiation of a white light to image the silica gel plate every 3 minutes , Continuously monitor the influence of different reaction times on the developing effect. Such as figure 1 As shown, within t...

Embodiment 2

[0037] (1) Spot the standard solutions containing 0.01mg / ml BHT and 0.01mg / ml TBHQ in a strip shape (6mm) on the silica gel plate to form 5 calibration points with a concentration gradient of 50-200 ng / zone, and make two parallel, and then develop the silica gel plate with toluene / ethyl acetate / methanol with a volume ratio of 9 / 1 / 1, dry it, and immerse it in the biological developing solution;

[0038] (2) Quantitative scanning by thin-layer scanner: place the silica gel plate after biological development in a thin-layer scanner for quantitative analysis, select the light source as deuterium lamp and tungsten lamp, fluorescence-reflection mode, and the emission wavelength is 530nm to obtain the optical density scan image as Figure 5 shown. After the scan, the scanned area is taken as the y-axis, and the amount of the target substance is taken as the x-axis to create a standard curve. The result is as Figure 6 shown.

Embodiment 3

[0040] (1) Simultaneous detection of BHT and TBHQ in soybean oil and sunflower oil, respectively.

[0041]Pretreatment of the edible oil sample: mix 1 g of the edible oil with 10 ml of methanol, shake in a vortex mixer for 3 minutes, centrifuge at 5000 rpm, filter through a 0.45-micron membrane, perform chromatographic separation, and refrigerate at 4°C.

[0042] (2) Using 0.5 MPa nitrogen gas as the carrier, use a 100 μl syringe (CAMAG) to accurately spot samples and antioxidant standards on a 10×10 cm silica gel plate with a 100 μl syringe (CAMAG). 8mm from the bottom, 12mm from the left end, and 1.7mm between the strips. After sample pointing, use the ADC-2 (CAMAG) developing instrument to develop. Before developing, inject 10 mL of mobile phase into another tank to make the cylinder reach a saturated state. Take 10 mL of the optimized developing solution (toluene / ethyl acetate / methanol=9 / 1 / 1 (v / v / v)), develop 60 mm upward, take it out, and place it on a flat heater at 50°...

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Abstract

The invention relates to a HPTLC-method for screening the synthesized phenolic antioxidants in oil and fat by bioautography, belonging to the field of food testing technology. According to the property that phenolic antioxidants changes color by removing the free radical groups from other reactants in a reaction system, the analytical method is established based on antioxidant color development. The testing sample first undergoes a derivative reaction, then using HPTLC as the open chromatographic separation carrier the sample is quantitatively scanned with a thin-layer chromatography scanner.The method of the invention had the advantage of short testing time and satisfactory accuracy. The testing result is generally consistent with that of HPLC.

Description

technical field [0001] The invention relates to an HPTLC-biological imaging screening method for synthesizing phenolic antioxidants from oil and belongs to the technical field of food detection. Background technique [0002] Oil oxidation caused by free radical chain reaction is one of the main reasons for the quality deterioration of oil-rich foods during processing, storage and shelf life. In addition to causing food rancidity and bad flavor, oil oxidation also produces a large amount of reaction by-products such as aldehydes and ketones, which poses a serious threat to the health of consumers. In order to prevent or slow down the oil oxidation, a large number of natural / synthetic antioxidants are added to the food matrix in the modern food industry. Compared with other types of antioxidants, synthetic phenolic antioxidants have received extensive attention due to their efficient free radical scavenging ability and tolerance to extreme temperature / pH environments, and hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 陈益胜徐学明王了谢正军杨哪吴凤凤金亚美金征宇
Owner JIANGNAN UNIV
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