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A method for rapid screening of fluorescent whitening agents in rice noodle products by high performance thin layer chromatography-mass spectrometry

A technology of high-efficiency thin-layer chromatography and fluorescent whitening agent, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., and can solve the problems of complicated sample preparation steps, low sensitivity and specificity, and expensive analysis costs

Active Publication Date: 2020-09-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the former has low sensitivity and specificity, while the latter has high requirements for analytical equipment, complicated sample preparation steps, and expensive analysis costs.

Method used

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  • A method for rapid screening of fluorescent whitening agents in rice noodle products by high performance thin layer chromatography-mass spectrometry
  • A method for rapid screening of fluorescent whitening agents in rice noodle products by high performance thin layer chromatography-mass spectrometry
  • A method for rapid screening of fluorescent whitening agents in rice noodle products by high performance thin layer chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of FWAs standard solution: use methanol: ethyl acetate = 1:9 as solvent, prepare standard solutions of FWA185 and FWA393 with a concentration of 0.01mg / mL, and then establish a standard curve by diluting the standard solution to 0.001 and 0.0001 mg / mL;

[0031] (2) High-efficiency thin-layer chromatographic separation: 4-8 μL of FWA185 and FWA393 mixed standards, wheat / rice flour and wheat / rice noodle samples were accurately spotted with Linomat 5, and after spotting was completed, the developer solution (toluene: acetic acid Ethyl ester=10:0.3 (v / v)) to unfold, the upward unfolding distance is 50 mm, after the unfolding is completed, take out the silica gel plate and place it on a flat heater at 60°C for 3 minutes to fully dry;

[0032] (3) Scanning and quantification with a thin-layer scanner: place the high-efficiency thin-layer chromatography plate in step (2) in a thin-layer scanner for scanning and quantification. The light source is a mercury lamp...

Embodiment 2

[0036] The specific implementation method is the same as in Example 1, the difference is that the selection of the mobile phase for development, after testing toluene:ethyl acetate=10:0.3 (v / v), the development effect is the best, and then the matrix effect experiment is carried out, using the same proportion of mobile phase. There is no obvious difference in the development effect, indicating that the sample matrix has no obvious interference with the development, and this mobile phase is used for the subsequent development of the standard addition recovery.

Embodiment 3

[0038] (1) Wheat and rice flour and wheat and rice noodles were tested with different spiked amounts.

[0039] Pretreatment of wheat and rice flour and wheat and rice noodles: Add 20 mL of ethyl acetate to 5 g of the sample of wheat and rice flour and chopped wheat and rice noodles, ultrasonically homogenize for 20 minutes, let stand for 5 minutes, and use a 5 mL syringe Aspirate 1 mL of the supernatant, filter it through a 0.45 μm organic membrane, and refrigerate it at 4°C.

[0040] The pretreatment of the sample solution of the recovery rate of the standard addition: add 20 mL of ethyl acetate to 5 g of wheat flour and chopped wheat noodles of the sample, ultrasonically homogenize for 20 min, let stand for 5 min, draw 1 mL of the supernatant with a 5 mL syringe, and Filter through a 0.45 μm organic membrane, add 15 μL 0.01 mg / mL FWA185 to the wheat flour extract, add 10 μL 0.01 mg / mL FWA185 and 10 μL 0.01 mg / mL FWA393 to the wheat noodle extract, and refrigerate at 4 °C.

[...

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Abstract

The invention discloses a method for rapidly screening fluorescent whitening agents in rice and flour products by utilizing high-performance thin-layer chromatography-mass spectrometry, and belongs tothe technical field of food detection. The method comprises the steps that to-be-detected samples are firstly spread on thin-layer chromatography, then thin-layer chromatography scanning is carried out, the high-performance thin-layer chromatography result is subjected to target strip elution under a visual condition, and finally in-situ mass spectrometry detection is carried out. According to the method for rapidly screening the fluorescent whitening agents in the rice and flour products by utilizing the high-performance thin-layer chromatography-mass spectrometry, the multiple samples can be detected through a single experiment at the same time, and high-flux screening is realized; the single-pass screening process from preparing the samples to obtaining the final result can be controlled within 1-2 hours, the limit of detection can reach 18-21 microgram / kg, a detection method can achieve the repeated RSD (3.4%, the adding standard recovery is 78.4%-97.7%), and therefore the methodhas the advantages of being rapid, accurate and economical; and meanwhile, a new idea is provided for plane high-performance thin-layer chromatography-mass spectrometry analysis based on an in-situ mass spectrometry detection method.

Description

technical field [0001] The invention relates to a method for quickly screening fluorescent whitening agents in rice noodle products by high-efficiency thin-layer chromatography-mass spectrometry, and belongs to the technical field of food detection. Background technique [0002] Fluorescent whitening agents (FWAs) are fluorescent dyes, or white dyes, which are also complex organic compounds. Its characteristic is that it can absorb ultraviolet light and emit blue light visible to the naked eye at the same time, which complements the yellow light on the substrate and has a whitening effect, making the substance visible to the naked eye present a strong white effect. Commonly used in some synthetic fiber, paper, soap and other industries. This advantage has prompted the application of FWAs as dyeing agents in the textile and paper industries. The traditional concept is that the whiter and finer the flour, the better the sensory effect of making traditional flour products. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 陈益胜黄彩虹徐学明张煌金征宇谢正军王萍舒蓝萍杨哪金亚美吴凤凤
Owner JIANGNAN UNIV
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