A kind of primer combination, probe combination and its application in detecting porcine virus, detection reagent, kit and detection method

Abstract

The invention discloses a combination of primers, a combination of probes and their application in detecting porcine viruses, a detection reagent, a kit and a detection method; the invention provides a combination of primers, a combination of probes and their application in detecting porcine viruses Application, detection reagents, kits and detection methods, establish a joint detection method for five kinds of porcine viruses, which can quickly detect five major swine diseases of ASFV, FMDV, CSFV, HP‑PRRSV and PRV wild virus, and can achieve high detection of five kinds of viruses Throughput screening improves detection efficiency and reduces reagent consumption, thereby saving costs, shortening detection time, and reducing workload; providing detection reagents that can realize joint detection of five porcine viruses, using optimized primer combinations and probe combinations, so that reagents and The kit using this reagent can specifically and sensitively detect five porcine viruses of ASFV, FMDV, CSFV, HP-PRRSV and PRV wild virus; it can simply and quickly detect five porcine virus infections with different types of nucleic acids in parallel in a single reaction tube, Realized single tube PCR to detect DNA virus and RNA virus at the same time.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer combination, a probe combination and their application in detecting porcine viruses, a detection reagent, a kit and a detection method. Background technique [0002] African swine fever (ASF) is an acute, febrile, highly contagious zoonotic disease of pigs caused by African swine fever virus (ASFV), characterized clinically by high fever and reticuloendothelial bleeding Characterized by high mortality and high mortality, the case fatality rate of susceptible pigs is as high as 100%. The World Organization for Animal Health (OIE) has listed African swine fever as a legally notifiable animal disease, and my country has listed it as a first-class animal disease. In August 2018, my country's first case of African swine fever was diagnosed, and then African swine fever broke out in many places, which brought a heavy blow to my country's pig breeding industry. ...

AI Technical Summary

Problems solved by technology

Although some scholars have established five or more target fluorescent quantitative PCR methods, they are all based on dye-based fluorescent PCR. Due to the problem of false positives, it is difficult to apply to clinical sample detection.

[0010] At present, there are many fluorescent quantitative PCR methods that can detect ASFV, FMDV, CSFV, HP-PRRSV and PRV wild virus alone, but there are no reports on the TaqMan probe five-fold fluorescent quantitative PCR method that can detect the above five porcine viruses simultaneously.

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