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A kind of I. spp. wswm1171 and its application in the control of potato tuber moth pupae

A technology of WSWM1171, potato cubes, applied in the field of microorganisms, can solve the problems of pesticide residues, threats to human health and environmental safety, environmental pollution, etc.

Active Publication Date: 2021-08-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive and long-term use of chemical pesticides can easily lead to resistance of pathogenic bacteria, and at the same time cause serious environmental pollution, pesticide residues and other problems, posing a serious threat to human health and environmental safety

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment one, the isolation of pathogenic bacteria, identification

[0031] 1.1 Materials and methods

[0032] 1.1.1 Collection location

[0033] The strain was collected from a Lepidoptera species in the limestone mountain rainforest (N21°57′55″, E101°12′22″, 763 meters above sea level) in Baka Xiaozhai, Menglun Town, Mengla County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province. On the chrysalis.

[0034] 1.1.2 Isolation culture and morphology observation

[0035] After the specimens were brought back to the laboratory, SDAY medium (glucose 40g, peptone 10g, yeast extract powder 10g, agar 20g, natural pH) was used for isolation and culture. After 7 days of culture, the conidia were transferred to a new medium for purification.

[0036] For morphological observation, use transparent glue with good viscosity to stick a small amount of mycelium at the edge of the colony, place it in 95% alcohol for fixation for 5 seconds, and then use cotton blue staining...

Embodiment 2

[0047] Embodiment 2, I. tenipedis WSWM1171 indoor virulence assay in potato tuber moth pupae

[0048] 2.1 Pathogenicity of infection of potato tuber moth pupae by highly virulent strains by different inoculation methods

[0049] 2.1.1 Source of tested insects

[0050] The potato tuber moths tested were collected in the potato planting area of ​​Banqiao Town, Xuanwei City, Yunnan Province (altitude 1967m, N26°05′52.3″, E104°04′27.5″). With reference to the feeding method of Rondon et al. (2010), spread a thin layer of sterilized fine sand in the plexiglass transparent insect cage (length × width × height = 40 × 40 × 40cm), and then place a layer of fresh potato pieces on it. , put into the field to collect larvae or adults, place temperature 26 ℃, humidity is 70%, artificial climate chamber (Ningbo Haishu Saifu Experimental Instrument Factory, model is RXZ-260B-30) of 16h of light time, allow its mass reproduction. A large number of eggs on the same day were collected, and af...

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Abstract

This application discloses Isera WSWM1171 and its application in the control of potato tuber moth pupae. The Isera WSWM1171 is preserved in the General Microbiology Center of China Microorganism Collection Management Committee, address: Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, 100101, date of deposit: June 4, 2018, deposit number CGMCC No.15689. The I. tenilis WSWM1171 provided by the application has strong pathogenicity to the pupae of the potato tuber moth, and only infects the pupae of Lepidoptera, which overcomes the defect that the entomogenous fungus cannot infect the pupae in the soil. The biological control of potato tuber moth pupae meets the needs of the development of green pest control, and can reduce environmental pollution caused by chemical pesticides, insect resistance, and the killing of natural enemies.

Description

technical field [0001] The application belongs to the technical field of microbes, and in particular, relates to I. tenipedes WSWM1171 and its application in the control of potato tuber moth pupae. Background technique [0002] Isaria tenuipes, formerly known as Paecilomyces tenuipes, belongs to Ascomycota, Pyrenomycetes, Hypocreales, Ergotaceae ( Clavicipitaceae), belonging to the genus Isaria, is a common entomopathogenic fungus of Lepidoptera insects. This fungus has host specialization and is more prevalent on Lepidoptera pupae. Forest ecosystems play an important role in regulating insect populations, and their secondary metabolites have unique functions in anti-tumor, anti-bacterial, anti-depressant and sleep-improving properties, and are widely used in traditional Chinese medicine. [0003] Potato tuber moth (Phthorimaea operculella Zeller) belongs to Lepidoptera (Lepidoptera), Gelechiidae (Gelechiidae), wheat stem moth (Phthorimaea), is a kind of worldwide distribut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 陈斌郑亚强肖关丽桂富荣和淑琪李正跃张凌英苏造堂
Owner YUNNAN AGRICULTURAL UNIVERSITY
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