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Detection method of fructo-oligosaccharide and raffinose in infant milk powder

A technology for fructooligosaccharides and raffinose, which is used in measuring devices, instruments, scientific instruments, etc., can solve the problems of simultaneous detection methods that have not been reported, unable to obtain component information, and complicated operation processes, reducing the number of on-machine detection. Cost, pre-processing cost reduction, high inspection efficiency

Active Publication Date: 2018-12-18
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the content of fructooligosaccharides in food is determined by indirect method, and the operation process of this method is complicated, only the total amount of oligosaccharides can be obtained, and the specific component information cannot be obtained.
[0006] At present, there is no report on the simultaneous detection of fructo-oligosaccharides and raffinose in infant formula milk powder

Method used

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  • Detection method of fructo-oligosaccharide and raffinose in infant milk powder
  • Detection method of fructo-oligosaccharide and raffinose in infant milk powder
  • Detection method of fructo-oligosaccharide and raffinose in infant milk powder

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Embodiment 1

[0050] 1. Instruments and materials

[0051] Waters H-CLASS ultra-high performance liquid chromatography equipped with ACQUITY UPLC ELS detector (Waters, USA); chromatographic column: Click XIon chromatographic column (150 mm×3.0 mm i.d, 3 µm).

[0052] The standard substances of kestose, kestose, and kestose pentaose were Japanese Wako standard substance set, with a purity of 99.9%. Raffinose was purchased from Sigma, with a purity of ≥99.5%, and stored at 4°C; acetonitrile (chromatographically pure, Fisher, USA); water is ultrapure water; nitrogen (>99.999%); C18 adsorbent (Agilent, USA). Organic microporous membrane (0.22 µm, purchased from Shanghai Anpu Scientific Instrument Co., Ltd.).

[0053] 2. Pretreatment

[0054] 2.1 Standard stock solution

[0055] Accurately weigh 100 mg (accurate to 0.1 mg) of each standard substance of fructo-oligosaccharides (kestose, kestose, and kestose pentaose) and raffinose in a 10 mL volumetric flask, dissolve in water and make to volu...

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Abstract

The invention discloses a detection method of fructo-oligosaccharide and raffinose in infant milk powder. The detection method comprises the following steps: (1) pretreatment; (2) standard solution configuration; (3) liquid chromatogram and regression equation acquisition; (4) qualitative analysis; and (5) quantitative computation by adopting external standard method quantification: computing to obtain the concentration of each of kestose, nytose, 1F-fructofuranosylnystose and / or raffinose by adopting a regression equation of the step (3) according to the area of a chromatographic peak in a sample chromatogram; the research preferably selects the novel hydrophilic material, and develops a qualitative quantification method for simply, quickly, accurately and simultaneously detecting fructo-oligosaccharide and raffinose in the infant formula milk powder by combining the high-performance liquid chromatogram technology.

Description

technical field [0001] The invention relates to a method for detecting fructooligosaccharides and raffinose in infant milk powder, and belongs to the technical field of food detection. Background technique [0002] Fructo oligosaccharide (FOS) is an oligosaccharide composed of sucrose and 1~3 fructose through β-(2,1) glycosidic bonds, including kestose (GF2), kestetose ( GF3), fructopentose (GF4) and other ingredients. FOS has the function of promoting the growth of intestinal bifidobacteria and regulating the balance of intestinal flora. Raffinose is a functional oligosaccharide composed of galactose, fructose and glucose, which also has a strong effect on the proliferation of bifidobacteria. Because the baby's intestinal development function is immature, it is prone to intestinal flora imbalance, and is infected by external viruses or bacteria, causing diarrhea and other diseases. Utilizing fructo-oligosaccharide and raffinose to regulate the function of intestinal flor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74
CPCG01N30/02G01N30/74
Inventor 张艳侠刘艳明薛霞徐向军于文江郭志谋闫竞宇别梅孙立臻
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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