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Construction of up-conversion weak light detector and application thereof in detecting glutathione

A glutathione, weak light technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high experimental requirements, time-consuming and labor-intensive

Inactive Publication Date: 2018-12-11
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require high experimental requirements and are time-consuming and labor-intensive.

Method used

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  • Construction of up-conversion weak light detector and application thereof in detecting glutathione
  • Construction of up-conversion weak light detector and application thereof in detecting glutathione
  • Construction of up-conversion weak light detector and application thereof in detecting glutathione

Examples

Experimental program
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Effect test

example 1

[0049] Based on the MPI-E electrochemiluminescence detector for transformation. Such as figure 1 As shown, the present invention provides an up-conversion weak light detector comprising a sample chamber 1 in the upper part. There is a detection pool at the bottom of the sample chamber 1. The detection pool is provided with a quartz window 2. The quartz window 2 is located in the sample chamber 1. A cuvette 3 is provided directly above the laser, the probe 4 of the laser is introduced into the sample chamber, and the position of the probe 4 is perpendicular to the cuvette 3 and then fixed. A vacuum chamber 5 is connected below the sample chamber 1, and a photomultiplier tube 6 is in the In the vacuum chamber 5 and facing the quartz window 2, the up-converted fluorescence passes through the cuvette 3 and the quartz window 2 at the bottom of the sample chamber, and then is collected through the photomultiplier tube 6, and then transferred to the computer system 7 for data process...

example 2

[0051] Preparation of α-phase OA-UCNPs: Take a clean three-necked flask, add 4 mL of oleic acid (OA), 15 mL of octadecene (ODE), prepared rare earth stearate (Y, Yb, Er) 1 mmol, NaF 20 mmol, heated under reflux to 135~145°C for 30min to dehydrate and degas to form a clear liquid. Then the temperature was raised rapidly, and the reaction temperature was maintained at 298~302°C for 30 minutes. After the reaction, cool to room temperature. The obtained product was centrifuged (11000 r / min), and the supernatant was discarded after the centrifugation, and the centrifuged precipitate was retained, and the precipitate was washed several times with cyclohexane, ethanol, and distilled water, and vacuum-dried at 70°C for later use.

[0052] Preparation of β-phase OA-UCNPs: Take a clean three-necked flask, add 12 mL of oleic acid (OA), 8 mL of octadecene (ODE), and 0.8 mmol of the prepared rare earth stearate (Y, Yb, Er) , NaF 28 mmol, heated under reflux to 135~145°C for 30 min to deh...

example 3

[0056] The performance of the up-conversion weak light detector prepared by example 1 is studied. At first, the signal-to-noise ratio of the two instruments is compared, as figure 2 As shown, a and b in the figure are the noise maps of the up-conversion weak light detector and the fluorescence spectrophotometer when testing the blank sample, respectively. Under the same test conditions (voltage: 500 V, excitation power: 0.25 W / cm 2 ), the baseline of the up-conversion weak light detector is a straight line, but the baseline of the fluorescence spectrophotometer has obvious jitter, indicating that the detection performance of the up-conversion weak light detector is better than that of the fluorescence spectrophotometer.

[0057] The α-phase UCNPs prepared in Example 2 were formulated into a 0.5 mg / mL aqueous solution, and the β-phase UCNPs were formulated into a 5 μg / mL aqueous solution, and the excitation power was 0.5 W / cm 2 , under the condition of a voltage of 500 V, det...

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Abstract

The invention discloses a construction of an up-conversion weak light detector and an application thereof in detecting glutathione and relates to the construction of an up-conversion weak light detection instrument. The detection instrument has the main advantages of (1) excellent detection performance and capability of detecting alpha-phase UCNPs with simple synthetic method and weak luminescenceduring the process of detecting UCNPs, and (2) low demand on UCNPs concentration and capability of detecting UCNPs in an approximately homogeneous system. In addition, the invention develops a biosensor taking a rare earth doped up-conversion nanometer material as a fluorescence probe and the application thereof in detecting GSH. The fluorescence response value of the sensor and the GSH concentration within the scope of 0.5-100uM have an excellent linear relation; the detection limit is 0.24uM; thus, the up-conversion weak light detector is expected to become a novel detection method for up-conversion luminescence and can be applied to more fields.

Description

technical field [0001] The invention relates to the construction of an up-conversion weak light detector, which belongs to the field of instrument analysis. [0002] The invention relates to using a rare earth-doped upconversion nanometer material as a fluorescent probe and using an upconversion weak light detector to detect glutathione (GSH), belonging to the field of analytical chemistry and nanotechnology. Background technique [0003] Glutathione (GSH) is a small molecular substance that exists in mammals and eukaryotic cells and contains a large amount of thiol tripeptides. It plays a central role in regulating redox balance, metabolism, detoxification and other biological processes. GSH is an important endogenous antioxidant, which can prevent the substances in cells from being damaged by active oxygen, and participate in metabolism and biochemical reactions at the same time. More importantly, the level of GSH is related to many diseases, such as HIV infection, psoria...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6486G01N2021/6432
Inventor 兰建明李春艳陈敬华吴芳刘映昕罗登旺方垚
Owner FUJIAN MEDICAL UNIV
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