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Preparation and application of a porcine Japanese encephalitis vaccine composition

A vaccine composition, Japanese encephalitis virus technology, applied in the directions of vaccines, veterinary vaccines, medical preparations containing active ingredients, etc. Effects of swine Japanese encephalitis, increasing antibody levels, and enhancing immune response effects

Active Publication Date: 2022-04-26
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although adjuvants have a history of several decades, there are very few adjuvants that have been certified for the prevention of porcine JE. With the rapid development of my country's pig industry, the situation of porcine JE prevention and control is becoming more and more severe. Traditional vaccine adjuvants can no longer solve the problem. Therefore, in addition to the development of high-efficiency and low-toxicity vaccines, the development of safe and efficient new vaccine compositions is more conducive to alleviating the severe situation of porcine encephalitis prevention and control, thereby reducing the pig industry. production costs and risks

Method used

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  • Preparation and application of a porcine Japanese encephalitis vaccine composition
  • Preparation and application of a porcine Japanese encephalitis vaccine composition
  • Preparation and application of a porcine Japanese encephalitis vaccine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 vaccine composition of the present invention

[0032] (1) Preparation of attenuated Japanese encephalitis Japanese encephalitis vaccine:

[0033] The JEV SCYA201201 attenuated strain was inoculated into BHK-21 cells (obtained by conventional cell culture), and the virus liquid was harvested. The specific method is as follows:

[0034] ①Select the BHK-21 cells that grow into a dense monolayer, wash twice with PBS, inoculate JEV SCYA201201 attenuated strain, the inoculum volume is 0.4mL, and incubate at 37°C.

[0035] ②Shake every 15 minutes during the incubation period, pour out the virus solution after 1 hour, add 6mL of maintenance solution (the maintenance solution is DMEM culture solution), and place at 37°C, 5% CO 2 Cultivate in a constant temperature incubator, and observe whether the cells have lesions every day.

[0036] ③ When 80% to 90% of the cells have lesions, freeze and thaw the cell culture repeatedly 3 times, pipette repe...

Embodiment 2

[0040] Example 2 The challenge protection of the vaccine composition of the present invention to mice

[0041] 1. Test material

[0042] Vaccine composition 1: take 10 ml of the vaccine composition prepared in Example 1, and the adjuvant concentration is 14.4 mg / mL (W / V).

[0043] Vaccine composition 2: Take 5ml of the double dilution of the stock solution prepared in Example 1, add 5ml of SCYA201201 attenuated strain cell culture solution, and the final concentration of adjuvant is 7.2mg / mL (W / V).

[0044] Vaccine composition 3: Take 5ml of the four-fold dilution of the stock solution prepared in Example 1, add 5ml of SCYA201201 attenuated strain cell culture solution, and the final concentration of adjuvant is 3.6mg / mL (W / V).

[0045] Vaccine composition 4: Take 5 ml of phosphate buffered saline (PBS, pH=7.2), add 5 ml of SCYA201201 attenuated strain cell culture medium, and the final concentration of adjuvant is 0 mg / mL (W / V).

[0046] 2. Test method

[0047] A total of ...

Embodiment 3

[0056] Embodiment 3 The antibody level detection of vaccine composition of the present invention

[0057] 1. Test material

[0058] Vaccine composition 1: take 10 ml of the vaccine composition prepared in Example 1, and the adjuvant concentration is 14.4 mg / mL (W / V).

[0059] Vaccine composition 2: Take 5ml of the double dilution of the stock solution prepared in Example 1, add 5ml of SCYA201201 attenuated strain cell culture solution, and the final concentration of adjuvant is 7.2mg / mL (W / V).

[0060] Vaccine composition 3: Take 5ml of the four-fold dilution of the stock solution prepared in Example 1, add 5ml of SCYA201201 attenuated strain cell culture solution, and the final concentration of adjuvant is 3.6mg / mL (W / V).

[0061] Vaccine composition 4: Take 5 ml of phosphate buffered saline (PBS, pH=7.2), add 5 ml of SCYA201201 attenuated strain cell culture medium, and the final concentration of adjuvant is 0 mg / mL (W / V).

[0062] 2. Test method

[0063] A total of 50 3-...

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Abstract

The invention provides a vaccine composition, which comprises polymer Carbomer 934P and Japanese encephalitis virus strain SCYA201201 (cell passage attenuated strain) cell culture fluid. Compared with live vaccines, the vaccine composition of the invention can greatly improve the immunogenicity, enhance the body's resistance to porcine Japanese encephalitis virus on the original basis, and provide new technical support for the prevention and control of the disease.

Description

technical field [0001] The invention relates to the preparation and application of a porcine Japanese encephalitis vaccine composition, belonging to the field of veterinary biological products. Background technique [0002] Porcine Japanese encephalitis is a serious zoonotic arboviral disease caused by Japanese encephalitis virus. Pigs are often susceptible when they are sexually mature, and the symptoms are depression, lethargy, reproductive impairment in pregnant sows and males. Porcine orchitis. [0003] Vaccine immunization is the main means to prevent the occurrence of porcine encephalitis B. Common porcine Japanese encephalitis vaccines include inactivated vaccines and live attenuated vaccines. Compared with inactivated vaccines, live attenuated vaccines have better immunogenicity, can induce solid immunity with less doses, do not require repeated immunizations, and have a long immunization period. [0004] However, the porcine JE vaccines currently used both at hom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K39/39A61P31/14
CPCA61K39/12A61K39/39A61P31/14A61K2039/552A61K2039/5254A61K2039/55555
Inventor 文翼平贺丽欣杨婕刘冬梅张锦王侨伍锐
Owner SICHUAN AGRI UNIV
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