Cadmium ion cutting type universal type isolating superfast amplification visualization sensor

Abstract

The invention provides a cadmium ion cutting type universal type isolating superfast amplification visualization sensor. The cadmium ion cutting type universal type isolating superfast amplification visualization sensor comprises 1, a cutting system, 2, a sPCR amplification system, and 3, a detection system containing ABTS color developing solution. According to a preparation method, skilful design of primers, templates, and probes is adopted, so that in the presence of cadmium ions, template superfast amplification is realized, a G-quadruplex is formed in an appropriate environment from amplification products; and the G-quadruplex peroxidase activity is adopted in color development, so that a difficult problem in the prior art that visualization detection of conventional PCR products is difficult to realize is solved, and rapid visualization detection on cadmium ions is realized. And in addition, the cadmium ion cutting type universal type isolating superfast amplification visualization sensor is high in specificity and sensitivity on cadmium ions, so that detection results are more objective and accurate.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a cadmium ion cutting type universal partition ultrafast amplification visualization sensor. Background technique [0002] Cadmium element symbol Cd, blue-white metal, belongs to Group IIB in the periodic table of elements, the atomic number of cadmium is 48, the atomic weight is 112.41, and the oxidation state is +1, +2. It is toxic, but due to its good flexibility and oxidation resistance , are widely used in industrial production. Cadmium can enter the organism through the respiratory and digestive tracts, and the cadmium entering the body can cause damage to a series of important organs such as the liver, kidney, bone, brain, and lung, and can also cause damage to the nervous, reproductive, and immune systems. China is the largest cadmium reserve and development country in the world, and it is also the largest cadmium consumer. In recent years, with the further developme...

AI Technical Summary

Problems solved by technology

However, these methods rely on large-scale instruments and special personnel to operate, and are limited in field and field detection

Chemical sensing methods represented by small organic molecules such as ethers, polyamines, and aromatic hydrocarbons have also been developed to a certain extent, but at present there are shortcomings such as low sensitivity, poor repeatability, and the detection needs to be carried out in organic solvents. Reliability and usability are not high

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