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Adsorbent for removing blood lipoprotein and preparation method thereof

A technology of lipoprotein and adsorbent, which is applied in the field of adsorbent for removing blood lipoprotein and its preparation, can solve the problems of insufficient application, high price, complicated operation, etc., and achieve improved adsorption effect, simple synthesis, and wide application prospects Effect

Active Publication Date: 2018-11-23
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although immunosorbents have high adsorption selectivity and good adsorption effect, the antibodies used are rare, expensive and difficult to store. Most of these products are treated by plasma adsorption. Although they can be reused, the operation is complicated and the consumables are expensive and expensive. Does not meet the needs of my country's national conditions
Although many scholars have devoted themselves to the study of lipid adsorbents in recent years, the results obtained are still not enough to be applied in practice.

Method used

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  • Adsorbent for removing blood lipoprotein and preparation method thereof
  • Adsorbent for removing blood lipoprotein and preparation method thereof
  • Adsorbent for removing blood lipoprotein and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] A method for preparing an adsorbent for removing blood lipoproteins, comprising the steps of:

[0032] 1) Grafted side chain

[0033] Put 1-10ml of solid porous cellulose microspheres into a 50ml or 100ml three-necked flask, add 10-30ml of 50% ethanol solution and 0.01g-0.1g of ammonium cerium nitrate, trigger for 15min, then add 0.5g- 3g monomer p-allyloxybenzaldehyde or 4-(3-methyl-2-butenyloxy)benzaldehyde was stirred and reacted at 45°C-70°C for 2h-6h. After the reaction is complete, take it out for suction filtration, wash the unreacted monomer and initiator with distilled water, and then use acetone Soxhlet extraction to remove the homopolymer. The grafting rate of the reaction is between 0.05% and 5%.

[0034] 2) Immobilized amino acids

[0035] Place the porous microspheres prepared in step 1 in a 50ml or 100ml round bottom flask, add 10-100ml of absolute ethanol and 0.1-1g of L-amino acid (the mass ratio of acidic amino acid to hydrophobic amino acid is 1:1)...

Embodiment 1

[0038] Place 1ml of the solid porous cellulose microspheres in a 50ml three-neck flask, add 20ml of 50% ethanol solution and 0.01g of ammonium persulfate and initiate for 15min, then add 0.5g of monomer p-allyloxybenzaldehyde dropwise , The reaction was stirred at 50°C for 4h. After the reaction is complete, take it out for suction filtration, wash the unreacted monomer and initiator with distilled water, and then use acetone Soxhlet extraction to remove the homopolymer.

[0039] Place the porous microspheres prepared above in a 50ml round bottom flask, add 10ml of absolute ethanol, 0.1g of L-aspartic acid and 0.1g of L-valine, and add 2 drops of glacial acetic acid. The reaction was stirred at reflux for 15h. After the reaction is completed, it is washed with distilled water to obtain LDL adsorbent 1.

Embodiment example 2

[0041] Place 5ml of the solid porous cellulose microspheres in a 100ml three-necked flask, add 50ml of 50% ethanol solution and 0.05g of cerium ammonium nitrate to initiate 15min, then add dropwise 1.5g of monomer 4-(3-methyl- 2-butenyloxy)benzaldehyde, stirred at 60°C for 3h. After the reaction is complete, take it out for suction filtration, wash the unreacted monomer and initiator with distilled water, and then use acetone Soxhlet extraction to remove the homopolymer.

[0042] Place the porous microspheres prepared above in a 50ml round-bottomed flask, add 30ml of absolute ethanol, 0.5g L-glutamic acid and 0.25g L-leucine, and add 2 drops of glacial acetic acid. The reaction was stirred at reflux for 16h. After the reaction is completed, wash with distilled water to obtain LDL adsorbent 2.

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Abstract

The invention discloses an adsorbent for removing blood lipoprotein and a preparation method thereof. Firstly, in the presence of an initiator, a grafting monomer is grafted on the surface of an adsorption carrier to form a connecting arm containing an aldehyde group, then the aldehyde group and Schiff base of amino acid molecules react to form a short chain containing acidic amino acids and hydrophobic amino acids, and by controlling the carrier content of acidic amino acids and hydrophobic amino acids, an LDL receptor and Apo B100-like binding domain and LDL are combined to reduce non-specific adsorption. The side chain grafting method adopted in the invention can increase a large number of binding sites and effectively load amino acids, and the side chain also functions as a connectingarm, thereby effectively reducing steric hindrance during adsorption and greatly improving the adsorption effect. The synthesis is simple and convenient, the raw materials are cheap and easily available, and thus the adsorbent has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an adsorbent for removing blood lipoprotein and a preparation method thereof. Background technique [0002] At present, cardiovascular disease, infectious disease and cancer are the three major causes of human death, among which 8 million to 10 million people die of cardiovascular disease every year, and cardiovascular disease has become the number one killer of human beings. The number of patients with cardiovascular diseases (coronary heart disease, stroke, heart failure, hypertension, etc.) in China is about 230 million, and the number of people who die from cardiovascular diseases is about 3 million every year, accounting for one-third of the total annual death toll in the country. . The disability rate of cardiovascular diseases is also very high. With the deepening of the aging of our country, the problem of cardiovascular diseases will become more and more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/36
CPCA61M1/3621B01J20/265
Inventor 姜建明梁达星
Owner 佛山市博新生物科技有限公司
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