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Roselle stem cell freeze-dried powder eye cream and preparation method thereof

A technology of roselle and stem cells, which can be used in pharmaceutical formulations, cosmetic preparations, dressing preparations, etc. It can solve the problems of high cost, high risk, and long time, so as to prolong the life of cells, protect the skin, and promote cell regeneration. Effect

Active Publication Date: 2018-11-02
GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the method for treating eye problems in the prior art, the time is long, the risk is high, the cost is high, and the eye skin care products only have the effect of replenishing water and moisturizing the surface, and have not fundamentally solved the problem of eye skin aging. This product uses roselle stem cell freeze-dried powder as the functional ingredient to stimulate the proliferation of human keratinocytes and increase the activity of SOD enzymes in the cells, thereby reducing the attack and damage of cells by oxygen free radicals induced by ultraviolet radiation, and adding polymerized almonds Protein, rich ether skin and squalane and other anti-wrinkle, anti-oxidation, skin firming and anti-slack ingredients aim to reduce cell death rate, repair cells, protect cells and tighten skin

Method used

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  • Roselle stem cell freeze-dried powder eye cream and preparation method thereof
  • Roselle stem cell freeze-dried powder eye cream and preparation method thereof
  • Roselle stem cell freeze-dried powder eye cream and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of a kind of roselle stem cell freeze-dried powder:

[0037] a. Take and cut the roselle root tip, clean and disinfect;

[0038] b. Cut the obtained roselle into a size of 5mm*5mm*5mm, and inoculate it to contain zinc sulfate 2.5mg / L, citric acid 75.5mg / L, glycine 80.0mg / L, indole acetic acid 1.5mg / L, sucrose 11.0mg / L, activated carbon 0.8g / L, aloin 4.5mg / ml and agar 3.2mg / L solid medium, the culture temperature is 25 ℃, the pH value is 6.5, cultivated for 14 days, and the cambium-derived roselle stem cells were obtained ;

[0039] c. Transfer the roselle stem cells derived from the cambium to a culture medium containing magnesium sulfate 181mg / L, citric acid 79mg / L, glycine 78.0mg / L, indole acetic acid 1.5mg / L, sucrose 33.0mg / L, aloin 2.5mg / L ml, medical stone granules 70.0mg / L and selenium ore granules 70.0mg / L liquid culture medium;

[0040] d. Move the cambium-derived roselle stem cells collected in step c into an airlift bioreactor...

Embodiment 2-4

[0059] Embodiment 2-4: a kind of roselle stem cell freeze-dried powder eye cream, including the following ingredients and their weight:

[0060] The composition and weight of table 1 embodiment 2-4 roselle stem cell freeze-dried powder eye cream

[0061]

[0062]

[0063] The roselle stem cell freeze-dried powder is prepared by the preparation method of the roselle stem cell freeze-dried powder described in Example 1.

[0064] The preparation method of roselle stem cell freeze-dried powder eye cream, the composition and weight thereof of embodiment 2-4 in table 1 are prepared according to the following steps respectively:

[0065] S1: Mix and dissolve propylene glycol, glycerin, butanediol, Carbopol 940 and preservatives in water, heat to 75°C, and stir until completely dissolved to obtain mixture A;

[0066] S2: Mix and dissolve dimethylsiloxane, caprylic triglyceride, isopropyl palmitate, synthetic squalane, and cetearyl alcohol ether in water, heat to 75°C, and stir ...

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Abstract

The invention belongs to the field of cosmetics and particularly relates to roselle stem cell freeze-dried powder eye cream and a preparation method thereof. The eye cream provided by the invention utilizes the functions of roselle stem cells for resisting oxidation, stimulating human skin keratinocyte proliferation and delaying senescence. The invention provides a roselle stem cell culture methodwith a short culture period. Roselle stem cells are freeze-dried into powder, and the content of the obtained roselle stem cell freeze-dried powder is high. In addition, the roselle stem cell freeze-dried powder is combined with components such as polymeric almond protein, fermiskin, dimethyl siloxane, caprylic / capric triglyceride, isopropyl palmitate, synthetic squalane, cetostearyl alcohol ether and L-proline to prepare the eye cream with functions of efficiently repairing eye damaged cells, resisting wrinkles, eliminating dark eye circles and under-eye puffiness and tightening eye skin.

Description

technical field [0001] The invention belongs to the technical field of daily cosmetics, and in particular relates to a roselle stem cell freeze-dried powder eye cream and a preparation method thereof. Background technique [0002] At present, eye problems such as dark circles, bags under the eyes, and wrinkles at the end of the eyes are mainly caused by internal factors: endocrine imbalance, mood swings, eye fatigue, cell aging, which hinder blood circulation in the eyes and accumulation of melanin; external factors: sun exposure, smoking, staying up late, habitual Strabismus causes the collagen in the dermis to be oxidized and broken. [0003] In order to address these two conditions, many treatment methods and products have been launched on the market. The treatment methods include Chinese medicine conditioning and Western medicine surgery. Chinese medicine conditioning mainly takes traditional Chinese medicine to regulate endocrine, thereby improving the surface muscles a...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/9789A61K8/64A61K8/31A61K8/34A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/31A61K8/345A61K8/645A61K8/99A61K2800/805A61K2800/84A61Q19/005A61Q19/02A61Q19/08A61K8/9789
Inventor 王一飞吴燕婷
Owner GUANGZHOU JINAN BIOMEDICINE RES & DEV CENT
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