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Aspartame gamma-L-Glu-D-Val as well as preparation method and application thereof

A technology of -l-glu-d-val and sweet peptide, applied in the field of sweet peptide γ-L-Glu-D-Val and its preparation, can solve the problem of γ-L-Glu-D-Val, etc. problems, to achieve good stability

Inactive Publication Date: 2018-10-30
GUANGDONG POLYTECHNIC OF ENVIRONMENTAL PROTECTION ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report that γ-L-Glu-D-Val has a natural sweetness similar to sucrose

Method used

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  • Aspartame gamma-L-Glu-D-Val as well as preparation method and application thereof
  • Aspartame gamma-L-Glu-D-Val as well as preparation method and application thereof
  • Aspartame gamma-L-Glu-D-Val as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Dissolve 300mM glutamine and 300mM valine in 100g water, adjust the pH value of the solution to 10.0 with 10 mol / l sodium hydroxide solution, add 0.4g glutaminase (Japan Amano Co., Ltd., the activity is 100GTU / g) React at 37°C for 12 hours, then adjust the pH to 7.0 with 4 mol / L hydrochloric acid, inactivate the enzyme at 90°C for 10 minutes, and perform sensory evaluation on the taste characteristics of the product. The taste characteristics before and after the reaction are as follows: figure 1 .

[0024] Depend on figure 1 It can be seen that after the reaction, the sweetness and umami taste of the reaction solution are significantly improved. It shows that the substance of the reaction solution has changed, and a substance with sweet taste is produced. In order to further confirm the change of substances, the reaction solution was used for high performance liquid chromatography to determine the product.

[0025] Quantitative analysis of HPLC liquid relative to t...

Embodiment 2

[0031] Add 0.01 g of purified γ-L-Glu-D-Val to black coffee solution or black tea containing an appropriate amount of sugar-free milk powder, and perform sensory evaluation on the product. The sensory evaluation experiment consisted of 7 males and 9 females aged 30-41 with sensory evaluation experience. The sensory evaluation results are shown in Figure 5 a and Figure 5b . Depend on Figure 5a It can be seen that when this sweet peptide is added to black coffee containing milk powder, compared with the blank group, the sweetness of the solution is significantly increased, and the bitterness and sourness are significantly reduced, which shows that this sweet peptide produces coffee mates similar to sucrose. The effect is to give black coffee a sweet taste. Such as Figure 5b , for black tea, after adding sweet peptides, a taste similar to milk tea appears, and the sweetness and silkiness increase. Judging from these two applications, this sweet peptide can be used to repla...

Embodiment 3

[0033] Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SWJS22 (General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number CGMCC No.8425) with 1% inoculum in the fermentation medium (the composition of the fermentation medium is: 4 % soybean meal, 2% bran, 2% corn flour), fermented at 37~39°C for 30h. The fermentation broth was centrifuged at 4°C and 10,000 r / min at high speed for 10 minutes, and the supernatant was filtered to obtain the crude glutaminase enzyme solution.

[0034]Dissolve 600mM glutamine and 300mM valine in 100g water, adjust the pH of the solution to 9.0 respectively, add 1g of crude enzyme solution, react at 40°C for 12h, adjust the pH to 7.0 with 4 mol / L hydrochloric acid, Inactivate the enzyme at 90°C for 10 min to obtain a reaction solution of γ-L-Glu-D-Val. Using XSelect HSS T3 5μm 4.6x 250mm analytical column, mobile phase A is 0.1% (V / V) formic acid-water solution; mobile phase B...

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Abstract

The invention discloses aspartame gamma-L-Glu-D-Val as well as a preparation method and the application thereof. The preparation method comprises the following specific steps: mixing L-glutamine withD-valine and dissolving into water, and adding glutaminase or gamma-glutamine transpeptidase which is 0.001 to 0.4 (w / w) percent of the mass of the solution; adjusting the pH value of the solution tobe 8.0 to 10.0 by using 10 mol / l of sodium hydroxide solution; after reacting at 25 to 50 DEG C for 3 to 12 hours, adjusting the pH value of the solution to 6 to 7 by using 4 mol / l of hydrochloric acid and deactivating enzyme at 90 DEG C for 10 minutes. The gamma-glutamine peptide (gamma-L-Glu-D-Val) prepared by the preparation method disclosed by the invention has good stability and natural sucrose-like sweetness; the sweetness of foods can be increased or improved when the gamma-glutamine peptide is used in foods.

Description

technical field [0001] The invention relates to a peptide with a taste effect and a preparation method thereof, in particular to a sweet taste peptide γ-L-Glu-D-Val and a preparation method and application thereof. Background technique [0002] Taste peptides are one of the important taste substances and flavor precursors. According to their different taste properties, they can be divided into sweet peptides, bitter peptides, sour peptides, salty peptides and umami peptides. Taste peptides with definite sequences of bitter, umami, sour, salty, sweet, etc. were isolated and identified from various foods such as cheese, wine, and meat successively. Since the discovery of the sweetness of L-aspartame-L-phenylalanine methyl ester, a large number of aspartame dipeptide, tripeptide, tetrapeptide and pentapeptide analogues have been synthesized for the purpose of extending sweetness. Types of flavor peptides. [0003] γ-Glutamyl peptide widely exists in a variety of animals, plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/027C07K1/16C12P21/02A23L27/30
CPCA23V2002/00A23L27/32C07K5/0205C12P21/02A23V2250/55
Inventor 张少敏刘慧燕沈会平
Owner GUANGDONG POLYTECHNIC OF ENVIRONMENTAL PROTECTION ENG
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