LC/MS method for the simultaneous determination of 45 prohibited drugs in health food for the three high populations

Abstract

The invention particularly relates to a high performance liquid chromatography-mass spectrometry combined method for simultaneously detecting 45 types of banned drugs in health-care food for people groups with three highs (high hypertension, hyperlipemia and hyperglycemia), and belongs to the technical field of detection of chemical drugs illegally added into health-care products. According to themethod, 5mmol.L<-1> of ammonium acetate liquor and acetonitrile are subjected to gradient elution on an Agilent Eclipse Plus C18 column, wherein the flow speed is 0.2mL.min<-1>; samples are ionized in the mode of positive and negative ions of electric-spray ion sources through mass spectrometry, and a multi-reaction monitor mode is applied to detection of the 45 types of chemical drugs. Three batches of positive samples from 300 batches of samples are detected in total, and detected drugs are phenformin, glibenclamide, hydrochlorothiazide and amlodipine. The high performance liquid chromatography-mass spectrometry combined method has the advantages that detection volume is high, neglected detection of the banned drugs can be avoided to a certain extent, and the method has a wide application range and a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of determination of illegally added chemicals in health care products, and in particular relates to a liquid chromatography-mass spectrometry method capable of simultaneously determining 45 kinds of prohibited drugs in health care food for three high populations. Background technique [0002] Hyperglycemia, hyperlipidemia, and hypertension are commonly known as the "three highs". These three constitute the main body of metabolic syndrome. At the same time, the incidence of metabolic syndrome will increase with age. On the other hand, hypertension, hyperlipidemia and hyperglycemia may exist alone or may be related to each other. For example, patients with hyperglycemia are likely to suffer from hyperlipidemia at the same time, and hyperlipidemia is the main factor in the formation and development of arteriosclerosis, which in turn leads to poor blood vessel elasticity and hypertension. Therefore, in order to...

AI Technical Summary

Problems solved by technology

Some criminals with high IQ who are familiar with pharmacology and drug effects illegally add chemicals across indications in order to evade relevant laws and regulations

For example, metformin, glibenclamide and other clinical first-line hypoglycemic prescription drugs that are mainly used for hypoglycemia are strictly monitored by the regulatory authorities as chemicals that are easily added illegally in supplementary hypoglycemic health foods, but they are not included in supplementary hypolipidemic health care products. Catalog of Chemicals Easily Added Illegally to Food

Images