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Pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof

A technology for Bacillus mycoides and pear tree roots, applied in the application, bacteria, biocide and other directions, can solve the problems of no high-efficiency, low-toxicity and non-environmental pollution biological methods, etc., and achieve the effect of improving quality and increasing yield

Inactive Publication Date: 2018-10-19
DEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control methods of pear tree brown spot include: doing a good job in clearing the garden, strengthening the management of pear orchards, strengthening cultivation management, and chemical control. There is no biological method with high efficiency, low toxicity and no environmental pollution for the control of pear trees brown spot disease

Method used

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  • Pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof
  • Pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof
  • Pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and identification of Bacillus mycoides Lzh-Z7

[0029] The strain Lzh-Z7 was isolated from the rhizosphere soil of pear trees infected with brown spot disease in the pear tree orchard of Dezhou City, Shandong Province. The specific method is as follows:

[0030] Rhizosphere growth-promoting bacteria were screened from plant rhizosphere soil by plate confrontation method. Take rhizosphere soil from the roots of infected plants and send it to the laboratory as soon as possible under low temperature conditions. The rhizosphere soil was put into sterile water for gradient dilution, and then spread on LB solid medium, cultured at 30°C for 24 hours, and picked a single colony. Inoculate the obtained single colony on the plant pathogenic bacteria to see if it produces a zone of inhibition and whether it has antagonistic biological activity. The strain Lzh-Z7 of the present invention was isolated through a large number of screening tests.

[0031] Bacill...

Embodiment 2

[0034] Example 2 Antagonism of Bacillus mycoides Lzh-Z7

[0035] The antagonism of Bacillus mycoides Lzh-Z7 was detected by plate confrontation method. The pear tree brown spot pathogen Mycosphaerella sentina (Fr) Schrorter was inoculated on PDA solid medium (200g of peeled potatoes, 20g of sucrose, 20g of agar, 1000mL of distilled water, natural pH), cultured at 30°C for 48hrs and then set aside. Bacillus mycoides Lzh-Z7 was inoculated into Gao's solid medium (soluble starch (20g), KNO 3 (1g), K 2 HPO 4 (0.5g), MgSO 4 ·7H 2 O (0.5g), NaCl (0.5g), FeSO 4 ·7H 2 O (0.01g), agar 20g, distilled water 1000mL, pH=7.4-7.6) upper 30 ℃ cultivates 48hr and inoculates to pear tree brown spot pathogenic bacteria both sides, 30 ℃ continues to cultivate 48-72hr, observe whether to produce antibacterial zone. The result is as figure 1 As shown, the results show that Bacillus mycoides Lzh-Z7 of the present invention has a good antagonistic effect.

Embodiment 3

[0036] Embodiment 3 Bacillus mycoides Lzh-Z7 microbial fertilizer and preparation method thereof

[0037] The concrete steps of preparation method are as follows:

[0038] (1) Preparation of microbial agent

[0039]The seed liquid of Bacillus mycoides Lzh-Z7 was inoculated in beef extract peptone liquid medium with a pH of 7.0, the inoculum amount was 1% (v / v), and fermented at 28°C and 200rpm for 48h to obtain the mushroom-shaped Bacillus Lzh-Z7 fermented liquid; the fermented liquid was added to sterilized wheat bran according to the inoculation amount of 5% by mass fraction, cultivated at 30°C for 3 days, and air-dried to obtain the microbial inoculant of Bacillus mycoides Lzh-Z7.

[0040] (2) Preparation of microbial fertilizer

[0041] Mix the mass ratio of the microbial agent obtained in step (1) with pig manure compost at a ratio of 1:10, adjust the water content to 50-60% after mixing, and carry out solid fermentation. The fermentation temperature does not exceed 50°...

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Abstract

The invention relates to pear rhizosphere growth promoting bacillus mycoides Lzh-Z7 and application thereof. The bacillus mycoides Lzh-Z7 is preserved in the General Microbial Center of the China Committee for Culture Collection of Microorganisms, the preservation number is CGMCC No.13491, the preservation date is September 23rd, 2016, and the address of the preservation institution is No.3 No.1 yard Beichen west road Chaoyang district, Beijing. The invention further discloses application of the bacillus mycoides Lzh-Z7, fermentation liquor and a microbial fertilizer containing the bacillus mycoides Lzh-Z7 in the aspect of preventing and treating pear root rot. Experiments show that the microbial fertilizer prepared from the bacillus mycoides Lzh-Z7 disclosed by the invention can effectively prevent and treat pear brown spot diseases, improve pear quality and yield and has very good application potential.

Description

technical field [0001] The invention relates to pear rhizosphere growth-promoting Bacillus mycoides Lzh-Z7, and also relates to the application of the bacteria in preventing and treating soil-borne diseases of pear trees, belonging to the field of biological control. Background technique [0002] Pear brown spot, also known as pear leaf spot, pear spot blight, and pear white spot, is caused by the fungus Mycosphaerella sentina (Fr) Schrorter. It mainly harms the leaves. In recent years, the outbreak has been aggravated year by year, causing a large number of early leaf fall, causing tree vigor to weaken, causing secondary flowering, and affecting the output of the next year. This poses a great threat to the development of the pear industry. At present, the prevention and control methods of pear tree brown spot include: doing a good job in clearing the garden, strengthening the management of pear orchards, strengthening cultivation management, and chemical control. There is n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00A01N63/00A01P3/00C12R1/07
CPCA01N63/00C05F3/00C12N1/20C12N1/205C12R2001/07C05F11/08
Inventor 李正华宋峰曾强成王吉华周耀旗于家峰冉坤沈亮陈梅
Owner DEZHOU UNIV
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