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A kind of composite microbial purification agent for river sewage purification and preparation method thereof

A technology of compound microorganism and purifying agent, which is applied in the field of compound microorganism purifying agent for river sewage purification and its preparation, can solve the problems of complicated preparation, high operation cost and insignificant effect, and achieves simple preparation method, economical cost and obvious effect. Effect

Active Publication Date: 2021-04-09
万创青绿(北京)环境科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a compound microbial purifying agent for purifying river sewage and its preparation method and its preparation method, so as to solve the problems of complex preparation, high operating cost and ineffective effect of the sewage purifying agent in the prior art

Method used

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preparation example Construction

[0024] The preparation method of above-mentioned compound microbial cleaning agent comprises:

[0025]A. Inoculate different high-purity strains in liquid medium, including Bacillus subtilis B.subtilis (Bacillus subtilis), Rhodopseudomonas palustris, Pseudomonas stutzeri, and denitrifying alternate unit cells The genus Alteromonas denitrificans and the genus Nitrosomonas europaea were inoculated with 1.5×10 9 ~3.5×10 9 cfu / mL, the number of inoculated bacteria is 2×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use;

[0026] The components of the liquid medium include: tryptone 8-12g / L, yeast extract 3-6g / L and NaCl 8-12g / L; preferably, the components of the liquid medium include: tryptone 10g / L , yeast extract 5g / L, NaCl 10g / L; the conditions for cultivating strains are: temperature is 22°C-28°C, pH is 6.7-7.3; Soil, trace element nutrient solution, straw slag powder, corn flour and fish meal are put into a stirring device a...

Embodiment 1

[0030] Inoculate different high-purity bacterial species in liquid culture medium, including Bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris, Pseudomonas stutzeri, denitrifying Alteromonas Alteromonas denitrificans and Nitrosomonas europaea, the inoculum number is 2×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use; put 340kg composite bacteria, 500kg diatomaceous earth, 10kg trace element nutrient solution, 50kg straw slag powder, 50kg corn flour and 50kg fish meal into Stir in a stirring device to obtain a mixture, wherein every kilogram of trace element nutrient solution contains 0.5g of ZnSO 4 , 2g of CaCl 2 , 2.5g of MnCl 2 4H 2 O, 0.5g of (NH 4 ) 6 Mo 7 o 24 4H 2 O, 0.1g of CuSO 4 ·5H 2 O and 0.5 g CoCl 2 ·6H 2 O, 3.8g of FeCl 2 4H 2 O, dry the mixture evenly stirred at a low temperature of 35°C to obtain a composite microbial purifier in the form of solid powder, which is easy to store and ...

Embodiment 2

[0035] Inoculate different high-purity bacterial species in liquid culture medium, including Bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris, Pseudomonas stutzeri, denitrifying Alteromonas Alteromonas denitrificans and Nitrosomonas europaea, the number of inoculum was 3.5×10 9 cfu / mL, mixed culture and enrichment, based on this to make composite bacteria, for later use; put 270kg composite bacteria, 550kg diatomaceous earth, 20kg trace element nutrient solution, 80kg straw residue powder, 30kg corn flour and 80kg fish meal into Stir in a stirring device to obtain a mixture, wherein every kilogram of trace element nutrient solution contains 0.3g of ZnSO 4 , 3g of CaCl 2 , 2g of MnCl 2 4H 2 O, 0.8g of (NH 4 ) 6 Mo 7 o 24 4H 2 O, 0.2g of CuSO 4 ·5H 2 O and 0.3 g CoCl 2 ·6H 2 O, 4.5g of FeCl 2 4H 2 O, dry the mixture evenly stirred at a low temperature of 35°C to obtain a composite microbial purifier in the form of solid powder, which is easy to sto...

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Abstract

The invention relates to a compound microbial purifying agent for purifying river sewage and a preparation method thereof. The compound microbial purifying agent comprises complex bacteria, and the complex bacteria comprise Bacillus subtilis, Rhodopseudomonas palustris and Pseudomonas stutzeri bacteria, denitrifying Alteromonas and Nitrosomonas. The preparation method of the compound microbial purifying agent for purifying river sewage is simple, can quickly and comprehensively purify water, can quickly and effectively reduce COD, N, TP, and chroma in river sewage, and can significantly improve the phenomenon of black and odorous water. Obviously, it has remarkable ecological benefits, is suitable for in-situ restoration of polluted river courses, and has the advantages of low cost and simple use.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a compound microbial purifying agent for purifying river sewage and a preparation method thereof. Background technique [0002] In recent decades, my country's urbanization process has developed rapidly, and with the rapid development of industrialization, people's demand for water resources has increased, and river water pollution incidents have occurred frequently, and the number of black and odorous water bodies in urban built-up areas Unabated, the supply of water resources has become more tense. Among them, the problem of eutrophication of water body due to excessive discharge of organic matter, nitrogen and phosphorus is particularly prominent. [0003] As an important decomposer in the aquatic ecosystem, microorganisms play an important role in the self-purification process of river water bodies. They can quickly and effectively degrade organic matter, nit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/38
CPCC02F3/34C02F2103/007C12N1/20
Inventor 张盛斌杨伟明
Owner 万创青绿(北京)环境科技有限公司
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