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Two-dimensional liquid chromatography detection method of compound Nanxingzhitong ointment

A two-dimensional liquid chromatography and detection method technology, which is applied in the field of two-dimensional liquid chromatography detection of Compound Nanxing Zhitong Ointment, can solve the problems of complex ingredients and cumbersome pretreatment methods of Compound Nanxing Analgesic Ointment, so as to improve the quality control level and simplify the The effect of sample pretreatment method and elimination of human error

Active Publication Date: 2021-04-30
JIANGSU KANION PHARMA CO LTD +1
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Problems solved by technology

Since the composition of Compound Nanxing Zhitong Ointment is extremely complex, and contains auxiliary materials such as vaseline and liquid paraffin, the pretreatment method of the sample is relatively cumbersome. The current quality standard of this preparation only uses the HPLC method to determine the content of hypoaconitine.

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Embodiment Construction

[0039] As mentioned above, the present invention aims to provide a method for detecting diester alkaloids in Compound Nanxing Zhitong Ointment.

[0040]Experimental equipment: Agilent 1290 Infinity II two-dimensional liquid chromatography system (including 1290 Infinity II high-speed pump, 1290 Infinity II autosampler, 1290 Infinity II column thermostat, 1290 Infinity II diode array detector, 1290 Infinity II binary pump for second-dimensional analysis, 1290 Infinity II diode array Detector, used for second-dimensional analysis, external valve drive), BUCHI R-3 rotary evaporator, KH-300DB numerical control ultrasonic cleaner, ME303E electronic balance, XS205DU electronic analytical balance.

[0041] Reagents and test drugs: Hypoaconitine (National Institute for Food and Drug Control, batch number 110798-201609), aconitine (National Institute for Food and Drug Control, batch number: 110720-201111), neoaconitine (National Institute for Food and Drug Control Research Institute, b...

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Abstract

The invention discloses a method for detecting diester-type alkaloids in compound Nanxingzhitong ointment. The method comprises: Step 1, preparation of the test product solution, extracting and removing impurities from the sample of Compound Nanxing Pain Relief Ointment to obtain the test product solution; Determination of diester alkaloids. The method proposed by the present invention has good accuracy and stability, effectively improves the separation degree of the components to be tested in the compound Nanxing Zhitong Ointment, greatly improves the detection sensitivity, and accurately detects the three diester-type alkaloids in the preparation. Quantitative analysis can improve the quality control level of products and provide theoretical support for clinical medication.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for detecting compound Nanxing Zhitong Ointment by two-dimensional liquid chromatography. Background technique [0002] In recent years, with the gradual maturity of commercial two-dimensional liquid chromatography instrument technology, two-dimensional liquid chromatography combined with multiple separation modes, such as heart-cutting, multi-heart-cutting and comprehensive two-dimensional liquid chromatography, have appeared, which can be divided into There are two ways, online and offline, in which the online two-dimensional liquid chromatography can selectively transfer the one-dimensional fraction to the second-dimensional chromatographic column for analysis, which can well solve the problem of limited separation ability of conventional chromatography. [0003] Compound Nanxing Pain Relief Ointment is composed of 12 herbs, including Aracnata si...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/46G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N30/46
Inventor 肖伟马阳李郭帅耿婷黄文哲王振中曹亮
Owner JIANGSU KANION PHARMA CO LTD
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