Quantum dot immunochromatography detection card and method for detecting naproxen through tertiary system competitive immunoassay process

Abstract

The invention relates to a quantum dot immunochromatography detection card and method for detecting naproxen through a tertiary system competitive immunoassay process. The detection card comprises a substrate, a detection antigen release pad, a quantum dot probe release pad, a chromatography film and a water absorbing pad, the detection antigen release pad contains detection antigen formed by coupling of ovalbumin and naproxen, the quantum dot probe release pad comprises a quantum dot probe marked with anti-ovalbumin antibody, the chromatography film is provided with a detection line and a quality control line, the detection line fixes anti-naproxen antibody, and the quality control line fixes ovalbumin antigen. The detection antigen is dissolved in a detection system from the release pad,the detection antigen, detection antibody and marking antibody form a tertiary system immune compound, and fluorescent detection signals are generated. Under detection reference action of the qualitycontrol line, whether samples contain naproxen or not is judged according to fluorescent signals on the detection line, so that signal intensity and stability of detection are improved, and detectionsensitivity and quantification accuracy are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health products and Chinese patent medicines, especially the quantum dot immunochromatography for the detection of naproxen by the ternary system immune competition method Test card and test method. Background technique [0002] The chemical name of naproxen is α-methyl-6-methoxy-2-naphthaleneacetic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatoid arthritis, adhesion spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, etc., but there are certain It is a prescription drug clinically and must be taken unde...

AI Technical Summary

Problems solved by technology

The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive

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