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Quantum dot immunochromatography detection card and method for detecting sulindac through ternary system competitive immunoassay process

A ternary system, immunochromatography technology, applied in measurement devices, analytical materials, biological tests, etc., can solve the problems of inability to uniformly prepare signal substances, insufficient stability, low detection signal strength and sensitivity, and achieve active protection. Favorable, good colloidal properties and kinetic properties, short detection time effect

Pending Publication Date: 2018-09-14
SHAOGUAN COLLEGE
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive

Method used

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  • Quantum dot immunochromatography detection card and method for detecting sulindac through ternary system competitive immunoassay process
  • Quantum dot immunochromatography detection card and method for detecting sulindac through ternary system competitive immunoassay process
  • Quantum dot immunochromatography detection card and method for detecting sulindac through ternary system competitive immunoassay process

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Embodiment Construction

[0029] See figure 1 , Which is the quantum dot immunochromatographic test card for the detection of sulindac by the ternary system immune competition method of this embodiment, including a PVC liner, and a test that is sequentially adhered to the PVC liner and partially overlapped between adjacent ones Antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and absorbent pad; the detection antigen release pad includes a detection antigen coupled by OVA and sulindac; the quantum dot probe The release pad contains a quantum dot probe labeled with an anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line fixes the anti-sulindac antibody, and the quality control line fixes the OVA antigen.

[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane with a thickness of 0.85 mm is used to fully absorb the 10 μg / mL det...

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Abstract

The invention relates to a quantum dot immunochromatography detection card and method for detecting sulindac through a tertiary system competitive immunoassay process. The detection card comprises a substrate, a detection antigen release pad, a quantum dot probe release pad, a chromatography film and a water absorbing pad, the detection antigen release pad contains detection antigen formed by coupling of ovalbumin and naproxen, the quantum dot probe release pad comprises a quantum dot probe marked with anti-ovalbumin antibody, the chromatography film is provided with a detection line and a quality control line, the detection line fixes anti-naproxen antibody, and the quality control line fixes ovalbumin antigen. The detection antigen is dissolved in a detection system from the release pad,the detection antigen, detection antibody and marking antibody form a tertiary system immune compound, and fluorescent detection signals are generated. Under detection reference action of the qualitycontrol line, whether samples contain sulindac or not is judged according to fluorescent signals on the detection line, so that signal intensity and stability of detection are improved, and detectionsensitivity and quantification accuracy are improved.

Description

Technical field [0001] The invention relates to the field of food and drug safety testing, in particular to a quantum dot immunochromatography that applies immunochemical testing technology to health care products and Chinese patent medicines illegally added chemicals, in particular to a ternary system immune competition method for detecting sulindac Test card and test method. Background technique [0002] The chemical name of sulindac is (Z)-2-methyl-1-[(4-methylsulfinylphenyl)methylene]-5-fluoro-1H-indene-3-acetic acid, which is non-steroidal Body anti-inflammatory drugs, with anti-inflammatory, analgesic and antipyretic effects, used for rheumatoid arthritis, adhesive spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation , All kinds of neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, but have certain side effects, are clinically prescribed drugs, and must be taken under th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/68
CPCG01N33/558G01N33/6854
Inventor 郭杰标章仕杰林秀玉江雪颜朱哲吴蓝洁张鹏
Owner SHAOGUAN COLLEGE
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