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Chitosan nano particle with carbon dioxide and acid responsiveness and preparation method of aqueous dispersion of chitosan nano particle

A chitosan nano and carbon dioxide technology, applied in the field of drug carrier and adsorption, can solve the problem of no stimulus response, and achieve the effect of functional enhancement, mild conditions and good water solubility.

Inactive Publication Date: 2018-09-07
JINGCHU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chitosan nanoparticles prepared by ion cross-linking basically do not have stimuli response.

Method used

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  • Chitosan nano particle with carbon dioxide and acid responsiveness and preparation method of aqueous dispersion of chitosan nano particle
  • Chitosan nano particle with carbon dioxide and acid responsiveness and preparation method of aqueous dispersion of chitosan nano particle
  • Chitosan nano particle with carbon dioxide and acid responsiveness and preparation method of aqueous dispersion of chitosan nano particle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Weigh 4.00g (2mmol) polyethylene glycol monomethyl ether (M n =2000) into a 100mL flask, add 50mLCH 2 Cl 2 And stir to dissolve, then add 1.50g 4-formylbenzoic acid, 1.92g EDC·HCl, 0.48g 4-dimethylaminopyridine (DMAP) and 2.8mL N-methylmorpholine; After stirring at room temperature for 24 hours, the reaction The solution was concentrated by rotary evaporation; the solid was precipitated in isopropanol, and after standing overnight, it was suction filtered, and the filter cake was washed three times with isopropanol; Glycol monomethyl ether (i.e. mPEG-CHO, M n =2000).

[0033] 2) Weigh 2.3g carboxymethyl chitosan and add it to a 100mL flask, then add 0.05g NaOH and 40mL distilled water, stir and dissolve to obtain carboxymethyl chitosan aqueous solution; add 1.5g mPEG-CHO (M n =2000) room temperature reaction for 24 hours, then get 8mL reaction solution, dropwise add the CaCl of 1.5mg / mL 2 2 mL of the solution was added dropwise and stirred rapidly, and after sta...

Embodiment 2

[0036] Different from Example 1, the NaOH added is changed to the sodium bicarbonate of the same mass, and the CaCl added dropwise is changed. 2 The concentration of the solution, the CaCl 2 The solution was increased to 1.8mg / mL, and the constant pressure dropping funnel was used for dropping at the same time, and it took 8.5 minutes to finish dropping, and finally prepared chitosan nanoparticles, which were characterized by a laser particle size analyzer. The Z average particle size was 237nm, polydisperse Coefficient PDI = 1.24.

Embodiment 3

[0038] 1) 16.0g (4mmol) polyethylene glycol monomethyl ether (mPEG, M n =4000) was dissolved in 50mL of dichloromethane, then added 3.0g p-formylbenzoic acid, 4.46g dicyclohexylcarbodiimide (DCC), 0.96g 4-dimethylaminopyridine (DMAP), and reacted at room temperature for 24 hours Afterwards, the reaction solution was concentrated, precipitated in isopropanol at 0° C., and the precipitate was collected by filtration. The filter cake was washed with isopropanol, and vacuum-dried at room temperature for 12 hours to obtain a white powder form of benzaldehyde-based polyethylene glycol monomethyl ether (mPEG -CHO, M n =4000).

[0039] 2) Take a 100mL flask, add 3.0g carboxymethyl chitosan and 40mL PBS buffer solution (pH=7.4), stir and dissolve to obtain carboxymethyl chitosan aqueous solution; then add 4.0g mPEG-CHO prepared in step 1) (M n =4000), feed nitrogen as a protective atmosphere, react at room temperature for 24 hours, then take 8mL of the reaction solution, and dropwis...

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Abstract

The invention discloses a chitosan nano particle with carbon dioxide and acid responsiveness and a preparation method of aqueous dispersion of the chitosan nano particle. The chitosan nano particle isprepared from carboxymethyl chitosan and calcium chloride by virtue of a co-precipitation method, wherein the carboxymethyl chitosan is modified by PEG by virtue of a benzimide bond. The aqueous dispersion of the chitosan nano particle can be stably stored in neutral and alkaline environments and then can be rapidly flocculated and precipitated when being stimulated by an acid environment or CO2.According to the invention, the aqueous dispersion of the chitosan nano particle is prepared by adopting an ion precipitation process, technology is simple, and conditions are mild; and the preparedaqueous dispersion of the chitosan nano particle has a better application prospect in the fields of intelligent drug carriers, adsorbing material and the like.

Description

technical field [0001] The invention belongs to the fields of drug carriers, adsorption and the like, and relates to a preparation method of chitosan nanoparticles with carbon dioxide and acid responsiveness and an aqueous dispersion thereof. Background technique [0002] Chitosan (CS for short) is a natural polymer polysaccharide obtained by deacetylating chitin. It is safe and non-toxic, has good antibacterial properties, tissue compatibility and biodegradability, and has been widely used in biomedicine. , tissue engineering and other fields. In recent years, with the rise of nanotechnology, chitosan nanoparticles have attracted widespread attention and have been used in many fields such as drug delivery, gene carriers, vaccine loading, fluorescent probes, and adsorption materials. [0003] At present, the most commonly used method for preparing chitosan nanoparticles is the ion cross-linking method. First, chitosan is dissolved in acetic acid solution, and its amino grou...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/36A61K47/10B01J20/24B01J20/28
CPCA61K9/5146A61K9/5161B01J20/24B01J20/28016Y02C20/40
Inventor 范伟伟李强杨洋
Owner JINGCHU UNIV OF TECH
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