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Detection method of content of polysaccharide in cordyceps sinensis mycelium powder and preparation thereof

A technology of fermented Cordyceps fungus powder and a detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack and inability to objectively and reasonably control the quality of medicinal materials and their preparations, and achieve good reproducibility and high detection sensitivity Effect

Pending Publication Date: 2018-09-04
JIANGXI GUOYAO PHARMA LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The detection method of the present invention has high detection sensitivity and good reproducibility, can objectively, comprehensively and accurately evaluate the quality of fermented Cordyceps fungus powder and its preparation polysaccharides, and can solve the problem of being unable to objectively and rationally control medicinal materials and their preparations due to lack of reference substances. The problem of preparation quality is of great significance to the quality control of fermented Cordyceps powder and its preparations

Method used

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  • Detection method of content of polysaccharide in cordyceps sinensis mycelium powder and preparation thereof
  • Detection method of content of polysaccharide in cordyceps sinensis mycelium powder and preparation thereof
  • Detection method of content of polysaccharide in cordyceps sinensis mycelium powder and preparation thereof

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Effect test

Embodiment 1

[0091] Determination of Polysaccharides in Jinshuibao Tablet Fermented Cordyceps Fungus Powder by "One Test and Multiple Reviews" Method

[0092] 1. Preparation of reference solution

[0093] Precisely weigh an appropriate amount of monosaccharide reference substance and place it in the same volumetric flask, add ultrapure water to dissolve and dilute to the mark, take an appropriate amount of monosaccharide solution and undergo PMP derivatization to obtain a mixed reference substance solution.

[0094] 2. Preparation of the test solution

[0095]Weigh an appropriate amount of Jinshuibao tablets, grind them into fine powder, and obtain crude polysaccharides by "water extraction and alcohol precipitation" method, then add Sevage reagent to remove protein, freeze-dry, and obtain fermented Cordyceps fungus powder polysaccharides. Take an appropriate amount of polysaccharide, and after acid hydrolysis and PMP derivatization, the test solution is obtained.

[0096] 3. Chromatogra...

Embodiment 2

[0113] Determination of Polysaccharides in Jinshuibao Capsule Fermented Cordyceps Fungus Powder by "One Test and Multiple Reviews" Method

[0114] 1. Preparation of reference solution

[0115] Precisely weigh an appropriate amount of monosaccharide reference substance and place it in the same volumetric flask, add ultrapure water to dissolve and dilute to the mark, take an appropriate amount of monosaccharide solution and undergo PMP derivatization to obtain a mixed reference substance solution.

[0116] 2. Preparation of the test solution

[0117] Weigh an appropriate amount of Jinshuibao capsules, obtain crude polysaccharides by "water extraction and alcohol precipitation", then add Sevage reagent to remove protein, freeze-dry to obtain fermented Cordyceps fungus powder polysaccharides. Take an appropriate amount of polysaccharide, and after acid hydrolysis and PMP derivatization, the test solution is obtained.

[0118] 3. Chromatographic conditions

[0119] Column: Kroma...

Embodiment 3

[0135] The detection method of the present invention is compared with the "Fingerprint Research of Fermented Cordyceps Fungus Powder Polysaccharides in Jinshuibao Capsules" published in the Journal of Chinese Herbal Medicine, Volume 48, Issue 16, August 2017. As an internal reference, in the absence of other monosaccharide reference substances, the content of each monosaccharide component in the polysaccharide of fermented Cordyceps fungus powder is determined to realize the quality control of fermented Cordyceps fungus fungus powder, and at the same time, it can avoid the problems caused by the determination of multi-component content. It can provide a new method for the quality control of fermented Cordyceps powder and its preparations.

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Abstract

The invention relates to a detection method of the content of polysaccharide in cordyceps sinensis mycelium powder and a preparation thereof. The detection method has the characteristics of high detection sensitivity and good repeatability. The method basically comprises the following steps: (1), preparation of a reference liquor: accurately weighing an appropriate amount of monosaccharide, addingultrapure water to dissolve the monosaccharide, and performing derivation, so as to obtain a mixed reference solution; (2), preparation of a test liquor: weighing an appropriate amount of cordyceps sinensis mycelium powder, obtaining crude polysaccharide through a water-extraction and alcohol-precipitation method, and performing acid hydrolysis and PMP derivation, so as to obtain a test solution;(3), calculation of relative correction factors: weighing the mixed relative correction factor obtained in the step (1), and calculating the relative correction factors of glucose to mannose, glucuronic acid, galacturonic, galactose, xylose, arabinose and fucose; (4), determination of the content of the test liquor: taking the test solution in the step (2), detecting the peak area of each monosaccharide through a high performance liquid chromatography, and calculating the contents of effective components through the relative correction factors.

Description

technical field [0001] The invention relates to a Chinese medicine fermented Cordyceps powder and a method for quality control of its preparations, in particular to a method for determining the content of polysaccharides in Jinshuibao Tablets and Jinshuibao Capsules. The method includes 8 kinds of monosaccharides The HPLC one-test and multiple-evaluation method for content determination of components. Background technique [0002] The complexity of traditional Chinese medicine components determines that a single component is difficult to express the quality of traditional Chinese medicine or Chinese patent medicines, and multi-component simultaneous quality control of traditional Chinese medicine is currently a widely recognized quality evaluation model for traditional Chinese medicine. The "one test and multiple evaluation" method is a new mode of multi-indicator quality evaluation suitable for the characteristics of traditional Chinese medicine. By measuring one component,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈丽华管咏梅朱卫丰杨明金晨刘小林
Owner JIANGXI GUOYAO PHARMA LLC
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