Method for preparing high-purity N-acetyl-D glucosamine

Abstract

The invention discloses a method for preparing high-purity N-acetyl-D glucosamine. The method comprises the following steps: preparing a solution from N-acetyl-D glucosamine, performing ultrafiltration with a ceramic membrane so as to obtain filtrate, putting a proper amount of activated carbon into the filtrate, filtering by using a sealed filtering machine so as to obtain a decolored liquid, performing electrodialysis desalting on the decolored liquid with an electrodialysis membrane so as to obtain a desalted liquid of which the conductivity is 5-50mu s / cm; performing evaporation concentration on the desalted liquid by using an evaporator so as to obtain a high-purity concentrated liquid; putting the high-purity concentrated liquid into a crystal cylinder, adding an organic solvent, andseparating a crystal so as to obtain N-acetyl-D glucosamine crystal slurry; pouring the crystal slurry into a centrifugal machine, performing solid-liquid separation so as to obtain a high-purity N-acetyl-D glucosamine wet crystal, and drying the wet crystal in a vacuum drying machine, so as to obtain the high-purity N-acetyl-D glucosamine of which the content is greater than or equal to 99.50%.Compared with the prior art, the method is low in cost, high in yield, short in process cycle and high in product purity.

Description

Technical field [0001] The invention relates to the technical field of biomedicine processing, in particular to a method for preparing high-purity N-acetyl-D glucosamine. Background technique [0002] N-acetyl-D glucosamine, English name: N-Acetyl-D-Glucosamine, molecular formula: C 8 h 15 NO 6 , Molecular weight: 221.21, N-acetylglucosamine is a new type of biochemical drug, and it is the unit of a variety of polysaccharides in the organism. It can improve digestion and absorption, reduce the intake of fat and cholesterol, lower blood pressure, regulate blood lipids, and promote ulcer healing. Enhance immunity, improve insulin utilization rate, help the prevention and treatment of diabetes, etc., at the same time, it can improve human immunity and protect liver and detoxify. The traditional method of producing N-acetyl-D glucosamine usually uses chitin as the starting material. The hydrolysis reaction produces D-glucosamine hydrochloride, that is, the β-1.4 glycosidic bond is co...

AI Technical Summary

Problems solved by technology

[0002] N-acetyl-D-glucosamine, English name: N-Acetyl-D-Glucosamine, molecular formula: C 8 h 15 NO 6 , Molecular weight: 221.21, N-acetylglucosamine is a new type of biochemical drug, and it is a unit of various polysaccharides in the body. It can improve digestion and absorption, reduce fat and cholesterol intake, lower blood pressure, regulate blood lipids, and promote ulcer healing. Enhance immunity, improve insulin utilization rate, be beneficial to the prevention and treatment of diabetes, etc. At the same time, it can improve human immunity and liver protection and detoxification. The traditional method of producing N-acetyl-D glucosamine usually uses chitin as the starting material. Hydrolysis reaction to obtain D-glucosamine hydrochloride, which is to completely hydrolyze and break the β-1.4 glycosidic bond into monosaccharides, and then react with acetic anhydride and triethylamine for acylation and a series of physical processes to obtain finished products. In this way, not only the process is cumbersome, but the yield is low, the cost is high and the sewage discharge is large.

The prior art also uses the principle of adsorption to separate impurities, and uses an adsorbent to adsorb impurities and colors in the product; and then uses the equilibrium relationship between liquid and solid to refine the recrystallization method. The recrystallization process used in the process route of this invention cannot achieve Low cost, high yield, high purity product requirements